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 Hoechst 33258染色液

  产品编号C1018
  产品包装: 50ml
  产品价格: 328.00元
产品编号 产品名称 产品包装 产品价格
C1018 Hoechst 33258染色液 50ml 328.00元

      Hoechst 33258,也称bisBenzimide H 33258或HOE 33258。分子式为C25H24N6O·3HCl,分子量为533.88,CAS
Number 23491-45-4。
      Hoechst 33258是一种可以穿透细胞膜的蓝色荧光染料,对细胞的毒性较低。
      Hoechst 33258染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33258也常用于普通的细胞核染色,或常规的DNA染色。
      Hoechst 33258的最大激发波长为346nm,最大发射波长为460nm;Hoechst 33258和双链DNA结合后,最大激发波长为352nm,最大发射波长为461nm。
      本Hoechst 33258染色液可直接用于固定细胞或组织的细胞核染色,也可直接用于活细胞或组织的细胞核染色。
包装清单:

产品编号 产品名称 包装
C1018 Hoechst 33258染色液 50ml
说明书 1份

保存条件:
      -20℃避光保存,一年有效。
注意事项:
      本Hoechst 33258 染色液的浓度经过碧云天的优化,确保可以满足各种常规染色的需要。如需使用特定浓度的Hoechst
33258,请选购碧云天的Hoechst 33258(C1011)。如需染色活细胞或培养的组织,请选购碧云天的Hoechst 33342活细胞染色液(100X) (C1027C1028C1029),效果更佳。
      荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。活细胞或组织染色后宜立即观察。
      为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。
      Hoechst 33258对人体有害,请注意适当防护。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 对于固定的细胞或组织:
a. 对于细胞或组织样品,固定后,适当洗涤去除固定剂。随后如果需要进行免疫荧光染色,则先进行免疫荧光染色,染色完毕后再按后续步骤进行Hoechst 33258染色。如果不需要进行其它染色,则直接进行后续的Hoechst 33258染色。
b. 对于贴壁细胞或组织切片,加入少量Hoechst 33258染色液,覆盖住样品即可。对于悬浮细胞,至少加入待染色样品体积3倍的染色液,混匀。室温放置3-5分钟。
c. 吸除Hoechst 33258染色液,用TBST、PBS或生理盐水洗涤2-3次,每次3-5分钟。
d. 直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时,会看到凋亡细胞的细胞核呈致密浓染,或呈碎块状致密浓染。
2. 对于活细胞或培养的组织:
a. 加入适当量Hoechst 33258染色液,必须充分覆盖住待染色的样品,通常对于六孔板一个孔需加入1ml染色液,对于96孔板一个孔需加入100微升染色液。
b. 在适宜于细胞培养的温度培养20-30分钟。弃染色液,用PBS或培养液洗涤2-3次即可进行荧光检测。细胞发生凋亡时,在荧光显微镜下观察会看到凋亡细胞的细胞核呈致密浓染,或呈碎块状致密浓染。

相关产品:

产品编号 产品名称 包装
C1002 DAPI 5mg/ml×0.2ml
C1005 DAPI染色液 10ml
C1006 DAPI染色液 50ml
C1011 Hoechst 33258 10mg
C1017 Hoechst 33258染色液 10ml
C1018 Hoechst 33258染色液 50ml
C1022 Hoechst 33342 10mg
C1025 Hoechst 33342染色液 10ml
C1026 Hoechst 33342染色液 50ml
C1027 Hoechst 33342活细胞染色液(100X) 0.1ml
C1028 Hoechst 33342活细胞染色液(100X) 0.5ml
C1029 Hoechst 33342活细胞染色液(100X) 3ml
相关产品请点击如下按钮:

使用本产品的文献:
1. Li-Xin Zhu, Jing Liu, You-Hua Xie, Yu-Ying Kong, Ye Ye, Chun-Lin Wang, Guang-Di Li, Yuan Wang.
Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells.
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C-myc antisense oligodexoynucleotides inhibite synoviocytes proliferation and induce synoviocytes apoptosis.
Chin J Rheumatol.2004 Feb;8(2):67-72.
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Effects of radizx astragali on the nitric oxides, free radicals and apoptosis of thymus in aging mice.
J Mudanjiang Med College.2004 May;25(4):14-5.
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Effect of angelica on apoptosis in brain cell and thymus index of aging mice.
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The study of apoptosis induced by methylene blue (MB) on C6 cell and MB enhances BCNU therapy of experimental rat C6 glioma.
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Construction of pcDNA3.0-ING4 eukaryotic expression vector and study on its function. 
Chinese Journal of Anatomy. 2005;28(4):383-6.
8. Yao G, Yang L, Hu Y, Liang J, Liang J, Hou Y. 
Nonylphenol-induced thymocyte apoptosis involved caspase-3 activation and mitochondrial depolarization
Mol Immunol .2006 Mar;43(7):915-26.
9. Tang W, Jiang MD, Li XA.
Effects of sp600125 on acetaldehydeinduced apoptosis of hepatic stellate cells and expression of Caspase-3 protein in rats.
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12.Jiang P, Yan PK, Chen JX, Zhu BY, Lei XY, Yin WD, Liao DF.
High density lipoprotein 3 inhibits oxidized low density lipoprotein-induced apoptosis via promoting cholesterol efflux in RAW264.7 cells.
Acta Pharmacol Sin. 2006 Feb;27(2):151-7.
13.Li HG, Liao AH, Ding XF, Zhou H, Xiong CL.
The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa.
Asian J Androl. 2006 May;8(3):301-6.
14.Mao WG, Liu ZL, Chen R, Li AP, Zhou JW.
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15.Xiong XL, Jia RH, Yang DP, Ding GH.
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Recombinant tissue factor pathway inhibitor induces apoptosis in cultured rat mesangial cells via its Kunitz-3 domain and C-terminal through inhibiting PI3-kinase/Akt pathway.
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The apoptotic effect of Sarsasapogenin from Anemarrhena asphodeloides on HepG2 human hepatoma cells
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Efficient inhibition of intraperitoneal human ovarian cancer growth and prolonged survival by gene transfer of vesicular stomatitis virus matrix protein in nude mice.
Gynecol Oncol. 2007 Mar;104(3):540-546.
19.Yao G, Ling L, Luan J, Ye D, Zhu P.
Nonylphenol induces apoptosis of Jurkat cells by a caspase-8 dependent mechanism.
Int Immunopharmacol. 2007 Apr;7(4):444-53.
20.Zhang Y, Ba Y, Liu C, Sun G, Ding L, Gao S, Hao J, Yu Z, Zhang J, Zen K, Tong Z, Xiang Y, Zhang CY.
PGC-1alpha induces apoptosis in human epithelial ovarian cancer cells through a PPARgamma-dependent pathway.
Cell Res. 2007 Apr;17(4):363-73.
21.Li X, Peng C, Li L, Ming M, Yang D, Le W.
Glial cell-derived neurotrophic factor protects against proteasome inhibition-induced dopamine neuron degeneration by suppression of endoplasmic reticulum stress and caspase-3 activation.
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22.Yan XB, Yang DS, Gao X, Feng J, Shi ZL, Ye Z.
Caspase-8 dependent osteosarcoma cell apoptosis induced by proteasome inhibitor MG132.
Cell Biol Int. 2007 Oct;31(10):1136-43.
23.Yao H, Tao Z, Ai M, Peng L, Wang G, He B, Li Y.
Raman spectroscopic analysis of apoptosis of single human gastric cancer cells.
Vibrational Spectroscopy .2008 Nov;50(2009):193–7.
24.Zhao X, Bai XJ, Feng JW, Dong YJ.
Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Neurocytes and Adipocytes Induced in vitro.
Scientia Agricultura Sinica. 2008;41(1):229-236.
25.Wu G, Liu ZS, Qian Q, Jiang CQ.
Effects of Berberine on the Growth of Hepatocellular Carcinoma Cell lines.
Medical Journal of Wuhan University.2008 Jan;29(1):102-105.
26.Xing C, Zhu B, Liu H, Yao H, Zhang L.
Class I phosphatidylinositol 3-kinase inhibitor LY294002 activates autophagy and induces apoptosis through p53 pathway in gastric cancer cell line SGC7901.
Acta Biochim Biophys Sin (Shanghai). 2008 Mar;40(3):194-201.
27.Wang Q, Wang Y, Liang C, Song J, Chen X.
Identification of a hydrophobic domain of HA2 essential to morphogenesis of Helicoverpa armigera nucleopolyhedrovirus.
J Virol. 2008 Apr;82(8):4072-81.
28.Xu HM, Jiang H, Wang J, Luo B, Xie JX.
Over-expressed human divalent metal transporter 1 is involved in iron accumulation in MES23.5 cells.
Neurochem Int. 2008 May;52(6):1044-51.
29.Chen P, Yang LL, Yang HS, Wang YS, Li G, Wu Y, Fang F, Liu K, Li J, Zhao X, Hu HZ, Wei YQ.
Synergistic antitumor effect of CXCL10 with hyperthermia.
J Cancer Res Clin Oncol. 2008 Jun;134(6):679-87.
30.Shen H, Qin H, Guo J.
Cooperation of metallothionein and zinc transporters for regulating zinc homeostasis in human intestinal Caco-2 cells.
Nutr Res. 2008 Jun;28(6):406-13.
31.Qin L, Zhang X, Zhang L, Feng Y, Weng GX, Li MZ, Kong QL, Qian CN, Zeng YX, Zeng MS, Liao DF, Song LB.
Downregulation of BMI-1 enhances 5-fluorouracil-induced apoptosis in nasopharyngeal carcinoma cells.
Biochem Biophys Res Commun. 2008 Jul 4;371(3):531-5.
32.Zhou J, Ye J, Zhao X, Li A, Zhou J.
JWA is required for arsenic trioxide induced apoptosis in HeLa and MCF-7 cells via reactive oxygen species and mitochondria linked signal pathway.
Toxicol Appl Pharmacol. 2008 Jul 1;230(1):33-40.
33.Yang X, Zhang Y, Zhang L, Zhang L, Mao J.
Silencing alpha-fetoprotein expression induces growth arrest and apoptosis in human hepatocellular cancer cell.
Cancer Lett. 2008 Nov 28;271(2):281-93.
34.Wang HQ, Xu YX, Zhao XY, Zhao H, Yan J, Sun XB, Guo JC, Zhu CQ.
Overexpression of F(0)F(1)-ATP synthase alpha suppresses mutant huntingtin aggregation and toxicity in vitro.
Biochem Biophys Res Commun. 2009;390(4):1294-8.
35.Zhang S, Wang J, Song N, Xie J, Jiang H.
Up-regulation of divalent metal transporter 1 is involved in 1-methyl-4-phenylpyridinium (MPP(+))-induced apoptosis in MES23.5 cells.
Neurobiol Aging. 2009 Sep; 30(9):1466-76.
36.Liu X, Zhang Z, Li P, Zhu L, Wang Y, Wang C.
Polypeptide from Chlamys farreri modulates UVB-induced activation of NF-kappaB signaling pathway and protection HaCaT cells from apoptosis.
Regul Pept. 2009 Feb 25;153(1-3):49-55.
37.Hu Z, Pan Y, Wang J, Chen J, Li J, Ren L.
Meso-tetra (carboxyphenyl) porphyrin (TCPP) nanoparticles were internalized by SW480 cells by a clathrin-mediated endocytosis pathway to induce high photocytotoxicity.
Biomed Pharmacother. 2009 Feb;63(2):155-64. 
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Curcumin induces apoptosis and inhibits growth of human Burkitt's lymphoma in xenograft mouse model.
Mol Cells. 2009 Mar;27(3):283-9.
39.Gong Y, Wu J, Huang Y, Shen S, Han X.
Nonylphenol induces apoptosis in rat testicular Sertoli cells via endoplasmic reticulum stress.
Toxicol Lett. 2009 Apr 25;186(2):84-95. 
40.Zhang SP, Zhou YJ, Liu Y, Cai YQ.
Effect of liquiritigenin, a flavanone existed from Radix glycyrrhizae on pro-apoptotic in SMMC-7721 cells.
Food Chem Toxicol. 2009 Apr;47(4):693-701. 
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The oncoprotein LMO3 interacts with calcium- and integrin-binding protein CIB.
Brain Res. 2009 Apr 10;1265:24-9.
42.Guo CJ, Pan Q, Li DG, Sun H, Liu BW.
miR-15b and miR-16 are implicated in activation of the rat hepatic stellate cell: An essential role for apoptosis.
J Hepatol. 2009 Apr;50(4):766-78. 
43.Zhu C, Huang H, Hua R, Li G, Yang D, Luo J, Zhang C, Shi L, Benovic JL, Zhou N.
Molecular and functional characterization of adipokinetic hormone receptor and its peptide ligands in Bombyx mori.
FEBS Lett. 2009 May 6;583(9):1463-8. 
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Acteoside protects human neuroblastoma SH-SY5Y cells against beta-amyloid-induced cell injury.
Brain Res. 2009 Aug 4;1283:139-47. 
45.Zeng X, Nan F, Liang C, Song J, Wang Q, Vlak JM, Chen X.
Functional analysis of the Autographa californica nucleopolyhedrovirus IAP1 and IAP2.
Sci China C Life Sci. 2009 Aug;52(8):761-70. 
46.Yu M, Cai L, Liang M, Huang Y, Gao H, Lu S, Fei J, Huang F.
Alteration of NRSF expression exacerbating 1-methyl-4-phenyl-pyridinium ion-induced cell death of SH-SY5Y cells.
Neurosci Res. 2009 Nov;65(3):236-44.
47.Liang W, Cheng J, Liu R, Wang JP, Mu JG, Wang QH, Wang HJ, Ma D.
Peptide corresponding to the C terminus of tissue factor pathway inhibitor inhibits mesangial cell proliferation and activation in vivo.
Peptides. 2009 Dec;30(12):2330-6. 
48.Zhang PY, Zhang WG, He AL, Wang JL, Li WB.
Identification and functional characterization of the novel acute monocytic leukemia associated antigen MLAA-34.
Cancer Immunol Immunother. 2009 Feb;58(2):281-90.
49.Chen Y, Chen Q, Lu J, Li FH, Tao YY, Liu CH.
Effects of Danggui Buxue Decoction () on lipid peroxidation and MMP-2/9 activities of fibrotic liver in rats.
Chin J Integr Med. 2009 Jan;15(6):435-41. >
50.Yan YJ, Zheng MZ, Chen ZL, Yu XH, Yang XX, Ding ZL, Xu L. 
Studies on preparation and photodynamic mechanism of chlorin P6-,15-N-(cyclohexyl)cycloimide (Chlorin-H) and its antitumor effect for photodynamic therapy in vitro and in vivo.
Bioorg Med Chem. 2010 Sep 1;18(17):6282-91.
51.Li G,Shi H,Huang H,Zhang Y,Wu K,Luo J,Sun Y, Lu J, L. Benovic J and Zhou N.
Internalization of the human nicotinic acid receptor GPR109A is regulated by Gi, GRK2, and arrestin3.
The Journal of Biological. 2010 Jul 16;285(29):22605-18.
52.Qian J, Jiang F, Wang B, Yu Y, Zhang X, Yin Z, Liu C.
Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells.
J Ethnopharmacol. 2010 Mar 24;128(2):438-45.
53.Lou C, Yang G, Cai H, Zou M, Xu Z, Li Y, Zhao F, Li W, Tong L, Wang M, Cai B.
2',4'-Dihydroxychalcone-induced apoptosis of human gastric cancer MGC-803 cells via down-regulation of survivin mRNA.
Toxicol In Vitro. 2010 Aug;24(5):1333-7.
54.Chen X, Tang W, Liu S, Yu L, Chen Z.
Thioredoxin-1 phosphorylated at T100 is needed for its anti-apoptotic activity in HepG2 cancer cells.
Life Sci. 2010 Aug 14;87(7-8):254-60.
55.Xu H, Yan Y, Li L, Peng S, Qu T, Wang B.
Ultraviolet B-induced apoptosis of human skin fibroblasts involves activation of caspase-8 and -3 with increased expression of vimentin.
Photodermatol Photoimmunol Photomed. 2010 Aug;26(4):198-204.
56.Ma J, Lu HY, Xia YK, Dong HB, Gu AH, Li ZY, Li Z, Chen AM, Wang XR, Wang SL.
BCL2 Ala43Thr is a functional variant associated with protection against azoospermia in a Han-Chinese population.
Biol Reprod. 2010 Oct;83(4):656-62.
57.Liu JT, Guo X, Ma WJ, Zhang YG, Xu P, Yao JF, Bai YD.
Mitochondrial function is altered in articular chondrocytes of an endemic osteoarthritis, Kashin-Beck disease.
Osteoarthritis Cartilage. 2010 Sep;18(9):1218-26.
58.Zhu W, Chang Y, Wu C, Han Q, Pei R, Lu M, Chen X.
The wild-type hepatitis C virus core inhibits initiation of antigen-specific T- and B-cell immuneresponses in BALB/c mice.
Clin Vaccine Immunol. 2010 Jul;17(7):1139-47.
59.Wang J, Han X, Yang H, Lu L, Wu Y, Liu X, Guo R, Zhang Y, Zhang Y, Li Q.
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60.Shen L, Lan Z, Sun X, Shi L, Liu Q, Ni J.
Proteomic analysis of lanthanum citrate-induced apoptosis in human cervical carcinoma SiHacells.
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61.Li K, Wang Y, Bai H, Wang Q, Song J, Zhou Y, Wu C, Chen X.
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Studies on preparation and photodynamic mechanism of chlorin P6-13,15-N-(cyclohexyl)cycloimide (Chlorin-H) and its antitumor effect for photodynamic
therapy in vitro and in vivo.

Bioorg Med Chem. 2010 Sep 1;18(17):6282-91.
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Downregulation of miR-21 modulates Ras expression to promote apoptosis and suppress invasionof Laryngeal squamous cell carcinoma.
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64.Zhu MT, Wang Y, Feng WY, Wang B, Wang M, Ouyang H, Chai ZF.
Oxidative stress and apoptosis induced by iron oxide nanoparticles in cultured human umbilicalendothelial cells.
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Small interfering RNA against the apurinic or apyrimidinic endonuclease enhances the sensitivityof human pancreatic cancer cells to gemcitabine in vitro.
J Dig Dis. 2010 Aug;11(4):224-30.
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Hyaluronic acid inhibits nitric oxide-induced apoptosis and dedifferentiation of articular chondrocytes in vitro.
INFLAMMATION RESEARCH.2010 Jul;59(7):519-30.
67.Du TT,Li L,Song N,Xie JX,Jiang H.
Rosmarinic Acid Antagonized 1-Methyl-4-Phenylpyridinium (MPP+)-Induced Neurotoxicity in MES23. 5 Dopaminergic Cells.
International Journal of Toxicology.2010 Dec;29(6):625-33.
68.Zheng J,Fang J, Yin YJ,Wang XC,Ren AJ,Bai J,Sun XJ,Yuan WJ,Lin L.
Leptin protects cardiomyocytes from serum‐deprivation‐induced apoptosis by increasing anti‐oxidant defence.
Clinical and Experimental Pharmacology and Physiology.2010 Oct;37(10):955–62.
69.Wang X,Xu J,Ju S,Ni H,Zhu J,Wang H.
Livin gene plays a role in drug resistance of colon cancer cells.
Clinical Biochemistry.2010 May;43(7-8):655–60.
70.Wang Y, Hong C, Zhou C, Xu D, Qu HB, Cheng Y.
Screening Antitumor Compounds Psoralen and Isopsoralen from Psoralea corylifolia L. Seeds.
Evid Based Complement Alternat Med. 2011;2011:363052.
71.Li X, Li G, Yu M, Yang Z, Sun J.
The effect ofwhole body low dose radiation on apoptosis of mice leukemia cell. 
Acta Acad Med Weifang. 2011 Oct;39(10):1018-29.
72.Wang Q, Jiang H, Fan Y, Huang X, Shen J, Qi H, Li Q, Lu X, Shao J.
Phosphorylation of the α-subunit of the eukaryotic initiation factor-2 (eIF2α) alleviates benzo[a]pyrene-7,8-diol-9,10-epoxide induced cell cycle arrest and apoptosis in human cells.
Environ Toxicol Pharmacol. 2011 Jan;31(1):18-24.
73.Wang F, Ding W, Wang J, Jing R, Wang X, Cong H, Wang Y, Ju S, Wang H.
Identification of microRNA-target interaction in APRIL-knockdown colorectal cancer cells.
Cancer Gene Ther. 2011 Jul;18(7):500-9.
74.Li DL, Liu JJ, Liu BH, Hu H, Sun L, Miao Y, Xu HF, Yu XJ, Ma X, Ren J, Zang WJ.
Acetylcholine inhibits hypoxia-induced tumor necrosis factor-α production via regulation of MAPKsphosphorylation in cardiomyocytes.
J Cell Physiol. 2011 Apr;226(4):1052-9.
75.Xu XL, Huang YJ, Wang YQ, Chen XF, Zhang W.
2,3,4',5-Tetrahydroxystilbene-2-O-β-d-glucoside inhibits platelet-derived growth factor-inducedproliferation of vascular smooth muscle cells by regulating the cell cycle.
Clin Exp Pharmacol Physiol. 2011 May;38(5):307-13.
76.Jiang QH, Liu L, Shen JW, Peel S, Yang GL, Zhao SF, He FM.
Influence of multilayer rhBMP-2 DNA coating on the proliferation and differentiation of MC3T3-E1cells seeded on roughed titanium surface.
J Biomed Mater Res A. 2012 Oct;100(10):2766-74.
77.He D, Ma X, Chen Y, Cai Y, Ru X, Bruce IC, Xia Q, Shi G, Jin J.
Luteolin inhibits pyrogallol-induced apoptosis through the extracellular signal-regulated kinasesignaling pathway.
FEBS J. 2012 May;279(10):1834-43.
78.Shi W, Nie D, Jin G, Chen W, Xia L, Wu X, Su X, Xu X, Ni L, Zhang X, Zhang X, Chen J.
BDNF blended chitosan scaffolds for human umbilical cord MSC transplants in traumatic braininjury therapy.
Biomaterials. 2012 Apr;33(11):3119-26.
79.Song J, Wang M, Huang H, Luo X, Deng F, Wang H, Hu Z.
Functional studies of per os infectivity factor 3 of Helicoverpa armigera nucleopolyhedrovirus.
J Gen Virol. 2012 Feb;93(Pt 2):374-82.
80.Yang S, Ma J, Xiao J, Lv X, Li X, Yang H, Liu Y, Feng S, Zhang Y.
Arctigenin Anti-Tumor Activity in Bladder Cancer T24 Cell Line Through Induction of Cell-CycleArrest and Apoptosis.
Anat Rec (Hoboken).2012 Aug;295(8):1260-6.
81.Yinghui Dong1, Hanhan Yao2, Zhihua Lin1,Dongli Zhu.
The effects of sperm–egg ratios on polyspermy in the blood clam,Tegillarca granosa.
Aquaculture Research.2012 Jun;43(1):44–52.
82.Zhong Q, Zhou Y, Ye W, Cai T, Zhang X, Deng DY.
Hypoxia-Inducible Factor 1-α-AA-Modified Bone Marrow Stem Cells Protect PC12 Cells fromHypoxia-Induced Apoptosis, Partially Through VEGF/PI3K/Akt/FoxO1 Pathway.
Stem Cells Dev.2012 Sep 20;21(14):2703-17.
83.Wu C, Deng W, Deng L, Cao L, Qin B, Li S, Wang Y, Pei R, Yang D, Lu M, Chen X.
Amino acid substitutions at positions 122 and 145 of hepatitis B virus surface antigen (HBsAg)determine the antigenicity and immunogenicity of HBsAg and influence in vivo HBsAg clearance.
J Virol. 2012 Apr;86(8):4658-69. 1.
84.Di S, Tian Z, Qian A, Li J, Wu J, Wang Z, Zhang D, Yin D, Brandi ML, Shang P.
Large gradient high magnetic field affects FLG29.1 cells differentiation to form osteoclast-like cells.
Int J Radiat Biol2012 Nov;88(11):806-13.
85.Zeng F, Ni E, Wang S, Lv S, Cao C, Zhang H, Lv H, Chen X, Yan J, Luo F.
A Monoclonal Antibody Against the Catalytic Domain of PTP1B.
Hybridoma (Larchmt). 2012 Jun;31(3):209-13.
86.Su W, Chen Y, Zeng W, Liu W, Sun H.
Involvement of Wnt signaling in the injury of murine mesenchymal stem cells exposed to X-radiation.
Int J Radiat Biol. 2012 Sep;88(9):635-41.
87.Li SZ,Li YH,Li JL,Liu C,Yin W,Zhao QX,Lin Z,Ji H and Yang HM.
Construction of pECFP-C1-CIRP and its effect on cold-induced apoptosis in mouse NIH/3T3 cells.
African Journal of Biotechnology.2012 Mar;11(21):4938-48.
88.Yuan XH, Lu CL, Yao N, An LS, Yang BQ, Zhang CL, Ma X.
Arsenic induced progesterone production in a caspase-3-dependent manner and changed redoxstatus in preovulatory granulosa cells.
J Cell Physiol. 2012 Jan;227(1):194-203.
89.Tang W, Zhang Y, Qian C, Yuan Z.
Induction and mechanism of apoptosis by hydroxycamptothecin in human Tenon's capsulefibroblasts.
Invest Ophthalmol Vis Sci. 2012 Jul 24;53(8):4874-80.
90.Zhang L, Ruan J, Yan L, Li W, Wu Y, Tao L, Zhang F, Zheng S, Wang A, Lu Y.
Xanthatin induces cell cycle arrest at G2/M checkpoint and apoptosis via disrupting NF-κB pathwayin A549 non-small-cell lung cancer cells.
Molecules. 2012 Mar 26;17(4):3736-50.
91.Jiang C, Zhang S, Liu H, Zeng Q, Xia T, Chen Y, Kuang G, Zhao G, Wu X, Zhang X, Wang A.
The role of the IRE1 pathway in PBDE-47-induced toxicity in human neuroblastoma SH-SY5Y cellsin vitro.
Toxicol Lett. 2012 Jun 20;211(3):325-33.
92.Li XJ, Zhu CD, Yu W, Wang P, Chen FF, Xia XY, Luo B.
Overexpression of Id3 induces apoptosis of A549 human lung adenocarcinoma cells.
Cell Prolif. 2012 Feb;45(1):1-8.
93.Zhou P, Li H, Wang H, Wang LF, Shi Z.
Bat severe acute respiratory syndrome-like coronavirus ORF3b homologues display differentinterferon antagonist activities.
J Gen Virol. 2012 Feb;93(Pt 2):275-81.  
94.Liu M, Yang S, Jin L, Hu D, Wu Z, Yang S.
Chemical Constituents of the Ethyl Acetate Extract of Belamcanda chinensis (L.) DCRoots and Their Antitumor Activities.
Molecules. 2012 May 24;17(5):6156-69.  
95.Wang JJ, Li YF, Jin YY, Wang X, Chen TX.
Effects of Epstein-Barr virus on the development of dendritic cells derived from cord bloodmonocytes: an essential role for apoptosis.
Braz J Infect Dis. 2012 Jan-Feb;16(1):19-26.
96.Zhao P, Han T, Guo JJ, Zhu SL, Wang J, Ao F, Jing MZ, She YL, Wu ZH, Ye LB.
HCV NS4B induces apoptosis through the mitochondrial death pathway.
Virus Res. 2012 Oct;169(1):1-7.
97.Muhammad Khan,Bin Zheng,Fei Yi,Azhar Rasul,Zhuyi Gu.
Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells.
Evidence-Based Complementary and Alternative Medicine,2012;2012:957568.
98.Wang PH, Liang JP, Gu ZH, Wan DH, Weng SP, Yu XQ, He JG.
Molecular cloning, characterization and expression analysis of two novel Tolls (LvToll2 and LvToll3) and three putative Spätzle-like Toll ligands (LvSpz1-3) from Litopenaeus vannamei.
Dev Comp Immunol. 2012 Feb;36(2):359-71.
99.Tao L, Chen J, Zheng Z, Meng J, Zhang Z, Chen Y, Luo H, Li H, Chen Z, Hu Q, Wang H.
H5N1 influenza virus-like particles produced by transient expression in mammalian cells induce humoral andcellular immune responses in mice.
Can J Microbiol. 2012 Apr;58(4):391-401.
100.Wang PH, Wan DH, Pang LR, Gu ZH, Qiu W, Weng SP, Yu XQ, He JG.
Molecular cloning, characterization and expression analysis of the tumor necrosis factor (TNF) superfamily gene, TNF receptor superfamily gene and lipopolysaccharide-induced TNF-α factor (LITAF) gene from Litopenaeus vannamei.
Dev Comp Immunol.2012 Jan;36(1):39-50.
101.Liu GY, Yang J, Dai F, Yan WJ, Wang Q, Li XZ, Ding DJ, Cao XY, Zhou B.
CuII ions and the stilbene-chroman hybrid with a catechol moiety synergistically induced DNA damage, and cell cycle arrest and apoptosis of HepG2 cells: an interesting acid/base-promoted prooxidant reaction.
Chemistry. 2012 Aug 27;18(35):11100-6. 
102.Chen YH, Zhao L, Jia XT, Li XY, Li CZ, Yan H, Weng SP, He JG.
Isolation and characterization of cDNAs encoding Ars2 and Pasha homologues, two components of the RNAinterference pathway in Litopenaeus vannamei.
Fish Shellfish Immunol. 2012 Feb;32(2):373-80.
103.
Ye Y, Li Z, Xing D.
Nitric oxide promotes MPK6-mediated caspase-3-like activation in cadmium-induced Arabidopsisthaliana programmed cell death. 
Plant Cell Environ.2013 Jan;36(1):1-15.
104.Huang J, Zhou L, Wang H, Luo J, Zeng L, Xiong K, Chen D.
Distribution of thrombospondins and their neuronal receptor α2δ1 in the rat retina.
Exp Eye Res. 2013 Jun;111:36-49.
105.Yu W, Sheng M, Xu R, Yu J, Cui K, Tong J, Shi L, Ren H, Du H.
Berberine protects human renal proximal tubular cells from hypoxia/reoxygenation injury via inhibitingendoplasmic reticulum and mitochondrial stress pathways.
J Transl Med. 2013 Jan 29;11:24. 
106.Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG.
Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeusvannamei.
PLoS One. 2013;8(2):e57456. 
107.Zhou L, Huang J, Wang H, Luo J, Zeng L, Xiong K, Chen D.
Expression of Glutamate and GABA during the Process of Rat Retinal Synaptic Plasticity Induced by Acute HighIntraocular Pressure.
Acta Histochem Cytochem. 2013 Feb 28;46(1):11-8. 
108.Zhang JF, Zhang JG, Kuai XL, Zhang H, Jiang W, Ding WF, Li ZL, Zhu HJ, Mao ZB.
Reactivation of the homeotic tumor suppressor gene CDX2 by 5-aza-2'-deoxycytidine-induced demethylation inhibits cell proliferation and induces caspase-independent apoptosis in gastric cancer cells.
Exp Ther Med. 2013 Mar;5(3):735-741. 
109.Tang XP, Tang GD, Fang CY, Liang ZH, Zhang LY.
Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells.
World J Gastroenterol. 2013 Mar 14;19(10):1582-92.
110.Yang S, Zhao Q, Xiang H, Liu M, Zhang Q, Xue W, Song B, Yang S.
Antiproliferative activity and apoptosis-inducing mechanism of constituents from Toona sinensis on humancancer cells.
Cancer Cell Int. 2013 Feb 9;13(1):12. 
111.Zhu D, Yu H, He H, Ding J, Tang J, Cao D, Hao L.
Spironolactone inhibits apoptosis in rat mesangial cells under hyperglycaemic conditions via the Wnt signallingpathway.
Mol Cell Biochem. 2013 Aug;380(1-2):185-93.
112.Yang YH, Chen K, Li B, Chen JW, Zheng XF, Wang YR, Jiang SD, Jiang LS.
Estradiol inhibits osteoblast apoptosis via promotion of autophagy through the ER-ERK-mTOR pathway.
Apoptosis. 2013 Nov;18(11):1363-75.
113.Li RL, Huang JJ, Shi YQ, Hu A, Lu ZY, Weng L, Wang SQ, Han YP, Zhang L, Hao CN, Duan JL.
Pulsed electromagnetic field improves postnatal neovascularization in response to hindlimb ischemia.
Am J Transl Res. 2015 Mar 15;7(3):430-44.
114.Fu X, Liu Y, Zhuang C, Liu L, Cai Z, Huang W.
Synthetic artificial microRNAs targeting UCA1-MALAT1 or c-Myc inhibit malignant phenotypes of bladder cancer cells T24 and 5637.
Mol Biosyst. 2015 May;11(5):1285-9.
115.Lu J, Cao Y, Cheng K, Xu B, Wang T, Yang Q, Yang Q, Feng X, Xia Q.
Berberine regulates neurite outgrowth through AMPK-dependent pathways by lowering energy status.
Exp Cell Res. 2015 Jun 10;334(2):194-206.
116.Wu D, Hu Q, Liu X, Pan L, Xiong Q, Zhu YZ.
Hydrogen sulfide protects against apoptosis under oxidative stress through SIRT1 pathway in H9c2 cardiomyocytes.
Nitric Oxide. 2015 Apr 30;46:204-12.
117.Wang X, Jiang P, Wang P, Yang CS, Wang X, Feng Q.
EGCG Enhances Cisplatin Sensitivity by Regulating Expression of the Copper and Cisplatin Influx Transporter CTR1 in Ovary Cancer.
PLoS One. 2015 Apr 30;10(4):e0125402.
118.Wu T, Wang S, Wu J, Lin Z, Sui X, Xu X, Shimizu N, Chen B, Wang X.
Icaritin induces lytic cytotoxicity in extranodal NK/T-cell lymphoma.
J Exp Clin Cancer Res. 2015 Feb 15;34:17.
119.Peng X, Zhuang DD, Guo QS.
Induction of S phase arrest and apoptosis by ethyl acetate extract from Tetrastigma hemsleyanum in human hepatoma HepG2 cells.
Tumour Biol. 2015 Apr;36(4):2541-50.
120.Dong M, Lu Y, Zou Z, Yang H.
Monoacylglycerol lipase inhibitor protects primary cultured neurons against homocysteine-induced impairments in rat caudate nucleus through COX-2 signaling.
Life Sci. 2015 Oct 1;138:64-71.
121.Xu L, Liu JH, Zhang J, Zhang N, Wang ZH.
Blockade of autophagy aggravates endoplasmic reticulum stress and improves Paclitaxel cytotoxicity in human cervical cancer cells.
Cancer Res Treat. 2015 Apr;47(2):313-21.
122.Dai JQ, Huang YG, He AN.
Dihydromethysticin kavalactone induces apoptosis in osteosarcoma cells through modulation of PI3K/Akt pathway, disruption of mitochondrial membrane potential and inducing cell cycle arrest.
Int J Clin Exp Pathol. 2015 May 1;8(5):4356-66.
123.Yan L, Wang C, Lin B, Liu J, Liu D, Hou R, Wang Y, Gao L, Zhang S, Iwamori M.
Lewis y enhances CAM-DR in ovarian cancer cells by activating the FAK signaling pathway and upregulating Bcl-2/Bcl-XL expression.
Biochimie. 2015 Jun;113:17-25.
124.Zuo N, Zheng X, Liu H, Ma X.
Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10653-61.
125.Wang W, Yan M, Ji Q, Lu J, Ji Y, Ji J.
Suberoylanilide hydroxamic acid suppresses hepatic stellate cells activation by HMGB1 dependent reduction of NF-κB1.
PeerJ. 2015 Nov 3;3:e1362.
126.Luo Q, Liu X, Zheng Y, Zhao Y, Zhu J, Zou L.
Ephrin-B2 mediates trophoblast-dependent maternal spiral artery remodeling in first trimester.
Placenta. 2015 May;36(5):567-74. 
127.Wu L, Dai J, Zhao X, Chen Y, Wang G, Li K.
Chloroquine enhances replication of influenza A virus A/WSN/33 (H1N1) in dose-, time-, and MOI-dependent manners in human lung epithelial cells A549.
J Med Virol. 2015 Jul;87(7):1096-103.
128.Jiang L, Wang L, Chen L, Cai GH, Ren QY, Chen JZ, Shi HJ, Xie YH.
As2O3 induces apoptosis in human hepatocellular carcinoma HepG2 cells through a ROS-mediated mitochondrial pathway and activation of caspases.
Int J Clin Exp Med. 2015 Feb 15;8(2):2190-6. 
129.Yang JA, Li JQ, Shao LM, Yang Q, Liu BH, Wu TF, Wu P, Yi W, Chen QX.
Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines.
Int J Clin Exp Med. 2015 Jun 15;8(6):10132-42. 
130.
Zhang K, Wang X, Wang C, Zheng H, Li T, Xiao S, Wang M, Fei C, Zhang L, Xue F. 
Investigation of quinocetone-induced mitochondrial damage and apoptosis in HepG2 cells and compared with its metabolites.
Environ Toxicol Pharmacol.2015 Mar;39(2):555-67.
131.Li W, Zhao XL, Shang SQ, Shen HQ, Chen X.>
Dual Inhibition of Cdc7 and Cdk9 by PHA-767491 Suppresses Hepatocarcinoma Synergistically with 5-Fluorouracil.
Curr Cancer Drug Targets.2015;15(3):196-204.
132.Wang LY, Li X, Han YZ.
Neuroprotection by epigallo catechin gallate against bupivacaine anesthesia induced toxicity involves modulation of PI3/Akt/PTEN signalling in N2a and SH-SY5Y cells.
Int J Clin Exp Med. 2015 Sep 15;8(9):15065-75.
133.Zhuang CL, Fu X, Liu L, Liu YC, Huang WR, Cai ZM.
Synthetic miRNA sponges driven by mutant hTERT promoter selectively inhibit the progression of bladder cancer.
Tumour Biol. 2015 Jul;36(7):5157-63.
134.Peng PA, Wang L, Ma Q, Xin Y, Zhang O, Han HY, Liu XL, Ji QW, Zhou YJ, Zhao YX.
Valsartan protects HK-2 cells from contrast media-induced apoptosis by inhibiting endoplasmic reticulum stress.
Cell Biol Int.2015 Dec;39(12):1408–17.
135.Zeng X, Tan X.
Epigallocatechin-3-gallate and zinc provide anti-apoptotic protection against hypoxia/reoxygenation injury in H9c2 rat cardiac myoblast cells.
Mol Med Rep. 2015 Aug;12(2):1850-6.
136.Jia G, Wang Q, Wang R, Deng D, Xue L, Shao N, Zhang Y, Xia X, Zhi F, Yang Y. 
Tubeimoside-1 induces glioma apoptosis through regulation of Bax/Bcl-2 and the ROS/Cytochrome C/Caspase-3 pathway.
Onco Targets Ther. 2015 Jan 30;8:303-11.
137.Nie GH, Luo L, Duan HF, Li XQ, Yin MJ, Li Z, Zhang W.
GALNT7, a target of miR-494, participates in the oncogenesis of nasopharyngeal carcinoma.
Tumour Biol.2016 Apr;37(4):4559–67.
138.Jiang Q, Liu Y, Duan D, Gou M, Wang H, Wang J, Li Q, Xiao R. 
Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica).
Biochimie. April 2016 Apr;123:7–19.
139.Han H, Yang J, Fei S, Liu Z, Zhu S, Dong Q, Gao Z, Wang S, Zhang J. 
Protective effect of carbachol postconditioning on hypoxia/reoxygenation-induced injury in human gastric epithelial cells.
Life Sci. 2016 Jan;144:234–42.
140.Zhao H, Ma T, Fan B, Yang L, Han C, Luo J, Kong L. 
Protective effect of trans-δ-viniferin against high glucose-induced oxidative stress in human umbilical vein endothelial cells through the SIRT1 pathway.
Free Radic Res. 2016 Jan;50(1):68-83.
141.Yu J, Xu H, Shen X, Jiang H. 
Ghrelin protects MES23.5 cells against rotenone via inhibiting mitochondrial dysfunction and apoptosis.
Neuropeptides. 2016 Apr;56:69-74.
140.Xing SF, Jang M, Wang YR, Piao XL.
A new dammarane-type saponin from Gynostemma pentaphyllum induces apoptosis in A549 humanlung carcinoma cells.
Bioorg Med Chem Lett. 2016 Apr 1;26(7):1754-9.
141.Huang L, Du B, Gong Y, Xu H, Lu J, Si X.
Effects of Exogenous Interleukin-9 on the Growth, Proliferation and Activity of Interstitial Cells of CajalCultured in vitro.
Digestion. 2016;94(3):154-165.
142.Pan C, Hu YF, Song J, Yi HS, Wang L, Yang YY, Wang YP, Zhang M, Pan MH, Lu C.
Effects of 10-hydroxycamptothecin on intrinsic mitochondrial pathway in silkworm BmN-SWU1 cells.
Pestic Biochem Physiol. 2016 Feb;127:15-20.
143.Liang Y, Ji J, Lin Y, He Y, Liu J.
The Ganglioside GM-1 Inhibits Bupivacaine-Induced Neurotoxicity in Mouse Neuroblastoma Neuro2aCells.
Cell Biochem Funct. 2016 Aug;34(6):455-62.
144.Ji BL, Xia LP, Zhou FX, Mao GZ, Xu LX.
Aconitine induces cell apoptosis in human pancreatic cancer via NF-κB signaling pathway.
Eur Rev Med Pharmacol Sci. 2016 Dec;20(23):4955-4964.
145.Luo P, Zhang WF, Qian ZX, Xiao LF, Wang H, Zhu TT, Li F, Hu CP, Zhang Z.
MiR-590-5p-meidated LOX-1 upregulation promotes Angiotensin II-induced endothelial cell apoptosis.
Biochem Biophys Res Commun. 2016 Mar 18;471(4):402-8.
146.Li J, Zhang Y, Luan H, Chen X, Han Y, Wang C.
l-carnitine protects human hepatocytes from oxidative stress-induced toxicity through Akt-mediatedactivation of Nrf2 signaling pathway.
Can J Physiol Pharmacol. 2016 May;94(5):517-25.
147.Liu X, Bi Y. 
Y-27632 Increases Sensitivity of PANC-1 Cells to EGCG in Regulating Cell Proliferation and Migration.
Med Sci Monit. 2016 Oct 3;22:3529-3534.
148.Jiang JM, Wang L, Gu HF, Wu K, Xiao F, Chen Y, Guo RM, Tang XQ.
Arecoline Induces Neurotoxicity to PC12 Cells: Involvement in ER Stress and Disturbance ofEndogenous H2S Generation.
Neurochem Res. 2016 Aug;41(8):2140-8.
149.Jiang Q, Liu Y, Duan D, Gou M, Wang H, Wang J, Li Q, Xiao R.
Anti-angiogenic activities of CRBGP from buccal glands of lampreys (Lampetra japonica).
Biochimie. 2016 Apr;123:7-19.
150.Wang W, Long L, Wang L, Tan C, Fei X, Chen L, Huang Q, Liang Z.
Knockdown of Cathepsin L promotes radiosensitivity of glioma stem cells both in vivo and in vitro.
Cancer Lett. 2016 Feb 28;371(2):274-84.
151.Wu C, Hu Y, Fan L, Wang H, Sun Z, Deng S, Liu Y, Hu C.
Ctenopharyngodon idella PKZ facilitates cell apoptosis through phosphorylating eIF2α.
Mol Immunol. 2016 Jan;69:13-23.
152.Li J, Li J, Aipire A, Gao L, Huo S, Luo J, Zhang F.
Phenylethanoid Glycosides from Cistanche tubulosa Inhibits the Growth of B16-F10 Cells both in Vitroand in Vivo by Induction of Apoptosis via Mitochondria-dependent Pathway.
J Cancer.2016 Sep 12;7(13):1877-87.
153.Zhu J, Jiang Y, Yang X, Wang S, Xie C, Li X, Li Y, Chen Y, Wang X, Meng Y, Zhu M, Wu R, Huang C, Ma X, Geng S, Wu J, Zhong C.
Wnt/β-catenin pathway mediates (-)-Epigallocatechin-3-gallate (EGCG) inhibition of lung cancer stemcells.
Biochem Biophys Res Commun. 2017 Jan 1;482(1):15-21.
154.Chen L, Wang L, Yan J, Ma C, Lu J, Chen G, Chen S, Su F, Wang W, Su X.
131I-labeled monoclonal antibody targeting neuropilin receptor type-2 for tumor SPECT imaging.
Int J Oncol. 2017 Feb;50(2):649-659.
155.Jiang Q, Li Q, Han J, Gou M, Zheng Y, Li B, Xiao R, Wang J.
rLj-RGD3 induces apoptosis via the mitochondrial-dependent pathway and inhibits adhesion, migrationand invasion of human HeyA8 cells via FAK pathway.
Int J ol Macromol. 2017 Mar;96:652-668.