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细胞相关> 细胞组织染色> 荧光染色
Hoechst 33342染色液
产品编号: C1026
产品包装:50ml
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价格:¥341.00元
产品简介
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产品编号 产品名称 产品包装 产品价格
C1025 Hoechst 33342染色液 10ml 101.00元
C1026 Hoechst 33342染色液 50ml 341.00元

Hoechst 33342,也称bisBenzimide H 33342或HOE 33342。分子式为C27H28N6O·3HCl·3H2O,分子量为615.99,CAS Number 23491-52-3。
Hoechst 33342是一种可以穿透细胞膜的蓝色荧光染料,对细胞的毒性较低。
Hoechst 33342染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33342也常用于普通的细胞核染色,或常规的DNA染色。
Hoechst 33342的最大激发波长为346nm,最大发射波长为460nm;Hoechst 33342和双链DNA结合后,最大激发波长为350nm,最大发射波长为461nm。
本Hoechst 33342染色液可直接用于固定细胞或组织的细胞核染色,也可直接用于活细胞或组织的细胞核染色。
包装清单:

产品编号 产品名称 包装
C1026 Hoechst 33342染色液 50ml
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
本Hoechst 33342 染色液的浓度经过碧云天的优化,确保可以满足各种常规染色的需要。如需使用特定浓度的Hoechst 33342,请选购碧云天的Hoechst 33342(C1022)。如需染色活细胞或培养的组织,请选购碧云天的Hoechst 33342活细胞染色液(100X) (C1027C1028C1029),效果更佳。
荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。活细胞或组织染色后宜立即观察。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。
Hoechst 33342对人体有害,请注意适当防护。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 对于固定的细胞或组织:
a.对于细胞或组织样品,固定后,适当洗涤去除固定剂。随后如果需要进行免疫荧光染色,则先进行免疫荧光染色,染色完毕后再按后续步骤进行Hoechst 33342染色。如果不需要进行其它染色,则直接进行后续的Hoechst 33342染色。
b.对于贴壁细胞或组织切片,加入少量Hoechst 33342染色液,覆盖住样品即可。对于悬浮细胞,至少加入待染色样品体积3倍的染色液,混匀。室温放置3-5分钟。
c. 吸除Hoechst 33342染色液,用TBST、PBS或生理盐水洗涤2-3次,每次3-5分钟。
d.直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时,会看到凋亡细胞的细胞核呈致密浓染,或呈碎块状致密浓染。
2. 对于活细胞或培养的组织:
a. 加入适当量Hoechst 33342染色液,必须充分覆盖住待染色的样品,通常对于六孔板一个孔需加入1ml染色液,对于96孔板一个孔需加入100微升染色液。
b. 在适宜于细胞培养的温度培养20-30分钟。弃染色液,用PBS或培养液洗涤2-3次即可进行荧光检测。细胞发生凋亡时,在荧光显微镜下观察会看到凋亡细胞的细胞核呈致密浓染,或呈碎块状致密浓染。

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