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细胞相关> 细胞组织染色> 荧光染色
DAPI
产品编号: C1002
产品包装:5mg/ml×0.2ml
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价格:¥248.00元
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产品编号 产品名称 产品包装 产品价格
C1002 DAPI 5mg/ml×0.2ml 248.00元

DAPI,即2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride,也称DAPI dihydrochloride,分子式为C16H15N5·2HCl,分子量为350.25,CAS Number 28718-90-3。
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。
DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI的最大激发波长为340nm,最大发射波长为488nm;DAPI和双链DNA结合后,最大激发波长为364nm,最大发射波长为454nm。
本DAPI溶液用水配制,浓度为5mg/ml。
用于细胞核染色时,推荐的DAPI工作浓度为0.5-10μg/ml。
包装清单:

产品编号 产品名称 包装
C1002 DAPI 5mg/ml×0.2ml
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。
DAPI对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

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