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 细胞凋亡-Hoechst染色试剂盒

  产品编号C0003
  产品包装: 100次
  产品价格: 353.00元
产品编号 产品名称 产品包装 产品价格
C0003 细胞凋亡-Hoechst染色试剂盒 100次 353.00元

      碧云天生产的细胞凋亡-Hoechst染色试剂盒(Hoechst Staining Kit)为您提供了一种经典而又快速简便的细胞凋亡检测方法。细胞发生凋亡时,染色质会固缩。所以Hoechst 33258染色后,在荧光显微镜下观察,正常细胞的细胞核呈正常的蓝色,而凋亡细胞的细胞核会呈致密浓染,或呈碎块状致密浓染,颜色有些发白。典型的Hoechst 33258染色后的细胞图片参考下图。


正常细胞核 刺激后有致密浓染的凋亡细胞

      只需25分钟即可完成细胞凋亡检测的整个过程。
      本试剂盒提供了固定液,染色液,及抗荧光淬灭封片液。
      本试剂盒对贴壁细胞,悬浮细胞和组织切片均适用。
      足够检测100个样品。
包装清单:

产品编号 产品名称 包装
C0003-1 固定液 50ml
C0003-2 Hoechst 33258染色液 50ml
C0003-3 抗荧光淬灭封片液 5ml
说明书 1份

保存条件:
      4℃保存,Hoechst 33258染色液需4℃避光保存。本试剂盒自订购之日起六个月内有效。
注意事项:
      需可以观察蓝色荧光的荧光显微镜或激光共聚焦显微镜。
      需PBS或0.9%NaCl溶液。
      需自备盖玻片与载玻片。盖玻片载玻片可以向碧云天订购。
      荧光物质均易发生淬灭,染色后的样品宜避光保存。可以向碧云天选购各种型号的载玻片存储盒
      在使用抗淬灭封片液的情况下可以减缓淬灭,但仍宜尽量避光。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.贴壁细胞
a.取洁净盖玻片在70%乙醇中浸泡5分钟或更长时间,无菌超净台内吹干或用无菌的PBS或0.9%NaCl等溶液洗涤三遍,再用细胞培养液洗涤一遍。将盖玻片置于六孔板内,种入细胞培养过夜,使约为50%-80%满。
b.刺激细胞发生凋亡后,吸尽培养液,加入0.5ml 固定液,固定10分钟或更长时间(可4℃过夜)。
c.去固定液,用PBS或0.9%NaCl 洗两遍,每次3分钟,吸尽液体。洗涤时宜用摇床,或手动晃动。
d.加入0.5ml Hoechst 33258染色液,染色5分钟。也宜用摇床,或手动晃动数次。
e.去染色液,用PBS或0.9%NaCl 洗两遍,每次3分钟,吸尽液体。洗涤时宜用摇床,或手动晃动。
f.滴一滴抗荧光淬灭封片液于载玻片上,盖上贴有细胞的盖玻片,让细胞接触封片液,尽量避免气泡。
g.荧光显微镜可检测到呈蓝色的细胞核。激发波长350nm左右,发射波长460nm左右,图谱请参考下图。
2.悬浮细胞
a.离心收集细胞样品于1.5ml离心管内,加入0.5ml 固定液,缓缓悬起细胞,固定10分钟或更长时间(可4℃过夜)。
b.离心去固定液,用PBS或0.9%NaCl 洗两遍,每次3分钟。洗涤期间手动晃动数次。
c.离心后吸去大部分液体保留约50μl液体,再缓缓悬起细胞,滴加至载玻片上,尽量使细胞分布均匀。
d.稍晾干,使细胞贴在载玻片上不易随液体流动。
e.均匀滴上0.5ml Hoechst 33258染色液,染色5分钟。用吸水纸从边缘吸去液体,微晾干。
f.去染色液,用PBS或0.9%NaCl 洗两遍,每次3分钟,吸尽液体。洗涤时宜用摇床,或手动晃动。
g.滴一滴抗荧光淬灭封片液于载玻片上,盖上一洁净的盖玻片,尽量避免气泡。
h.荧光显微镜可检测到呈蓝色的细胞核。激发波长350nm左右,发射波长460nm左右,图谱请参考下图。
3.组织切片
a.常规包埋切片后,根据切片的不同类型,处理至可以用于免疫组化染色。
b.PBS或0.9%NaCl 洗两遍,每次3分钟,吸尽液体。洗涤时宜用摇床,或手动晃动。可在六孔板中操作。
c.加入0.5ml Hoechst 33258染色液,染色5分钟。也宜用摇床,或手动晃动数次。
d.去染色液,用PBS或0.9%NaCl 洗两遍,每次3分钟,吸尽液体。洗涤时宜用摇床,或手动晃动。
e.小心将切片置于载玻片上,滴一滴抗淬灭封片液,盖上一洁净的盖玻片,尽量避免气泡。
f.荧光显微镜可检测到呈蓝色的细胞核。激发波长350nm左右,发射波长460nm左右,图谱请参考下图。

                                     Hoechst 33258的吸收光谱和发射光谱,左侧峰为吸收光谱,右侧峰为发射光谱。

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Pseudolaric acid B induces apoptosis via proteasome-mediated Bcl-2 degradation in hormone-refractory prostate cancer DU145 cells.
Toxicol In Vitro. 2012 Jun;26(4):595-602.
60.Wang YB, Wang JJ, Wang SH, Liu SS, Cao JY, Li XM, Qiu S, Luo JH.
Adaptor protein APPL1 couples synaptic NMDA receptor with neuronal prosurvival phosphatidylinositol 3-kinase/Akt pathway.
J Neurosci. 2012 Aug;32(35):11919-29.
61.Huang WT, Liu J, Liu JF, Hui L, Ding YL, Chen SW.
Synthesis and biological evaluation of conjugates of deoxypodophyllotoxin and 5-FU as inducer of caspase-3and -7.
Eur J Med Chem. 2012 Mar;49:48-54.
62.Shen A, Chen Y, Hong F, Lin J, Wei L, Hong Z, Sferra TJ, Peng J.
Pien Tze Huang suppresses IL-6-inducible STAT3 activation in human colon carcinoma cells through induction ofSOCS3.
Oncol Rep. 2012 Dec;28(6):2125-30.
63.Fang Y, Mo X, Luo Y, Lu Y.
BAX gene over-expression via nucleofection to induce apoptosis in human lens epithelial cells.
Exp Biol Med (Maywood). 2012 Sep;237(9):1000-6. 
64.Wang J, Bai X, Chen Y, Zhao Y, Liu X.
Homocysteine induces apoptosis of rat hippocampal neurons by inhibiting 14-3-3ε expression and activatingcalcineurin.
PLoS One. 2012;7(11):e48247.
65.Hu B, An HM, Shen KP, Xu L, DU Q, Deng S, Wu Y.
Liver Yin deficiency tonifying herbal extract induces apoptosis and cell senescence in Bel-7402 humanhepatocarcinoma cells.
Exp Ther Med. 2012 Jan;3(1):80-86.
66.Zhou Q, Xiong Y, Chen Y, Du Y, Zhang J, Mu J, Guo Q, Wang H, Ma D, Li X.
Effects of tissue factor pathway inhibitor-2 expression on biological behavior of BeWo and JEG-3 cell lines.
Clin Appl Thromb Hemost. 2012 Sep;18(5):526-33.
67.Jin M, Wang J, Xiao R, Liu X, Wu F, Pang Y, Feng B, Yang D, Li Q.
Effects of the recombinant toxin protein rLj-RGD3 in multidrug-resistant human breast carcinoma cells.
Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):455-61.
68.Xu Y, Yang S, Huang J, Ruan S, Zheng Z, Lin J.
Tgf-β1 induces autophagy and promotes apoptosis in renal tubular epithelial cells.
Int J Mol Med. 2012 May;29(5):781-90. 
69.Hu Z, Bian X, Liu X, Zhu Y, Zhang X, Chen S, Wang K, Wang Y.
Honokiol protects brain against ischemia-reperfusion injury in rats through disrupting PSD95-nNOS interaction.
Brain Res. 2013 Jan;1491:204-12.
70.Kang J, Zhao G, Lin T, Tang S, Xu G, Hu S, Bi Q, Guo C, Sun L, Han S, Xu Q, Nie Y, Wang B, Liang S, Ding J, Wu K.
A peptide derived from phage display library exhibits anti-tumor activity by targeting GRP78 in gastric cancermultidrug resistance cells.
Cancer Lett. 2013 Oct 10;339(2):247-59. 
71.Li C, Zhang X, Cheng L, Dai L, Xu F, Zhang J, Tian H, Chen X, Shi G, Li Y, Du T, Zhang S, Wei Y, Deng H.
RNA interference targeting human FAK and EGFR suppresses human non-small-cell lung cancer xenograftgrowth in nude mice.
Cancer Gene Ther. 2013 Feb;20(2):101-8. 
72.Liu L, Shen A, Chen Y, Wei L, Lin J, Sferra TJ, Hong Z, Peng J.
Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer.
Oncol Rep. 2013 Aug;30(2):897-903. 
73.Lu XL, Zeng J, Chen YL, He PM, Wen MX, Ren MD, Hu YN, Lu GF, He SΧ.
Sinomenine hydrochloride inhibits human hepatocellular carcinoma cell growth in vitro and in vivo: involvementof cell cycle arrest and apoptosis induction.

Int J Oncol. 2013 Jan;42(1):229-38. 
74.Yu W,Sheng M, Xu R, Yu J, Cui K, Tong J,Shi L,Ren H.
Berberine protects human renal proximal tubular cells from hypoxia/reoxygenation injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways.
Journal of Translational Medicine. 2013 Jan 29;11:24.
75.Xu Y, Ruan S, Wu X, Chen H, Zheng K, Fu B.
Autophagy and apoptosis in tubular cells following unilateral ureteral obstruction are associated withmitochondrial oxidative stress.
Int J Mol Med. 2013 Mar;31(3):628-36. 
76.Feng C, Xu Z, Li Z, Zhang D, Liu Q, Lu L.

Down-regulation of Wnt10a by RNA interference inhibits proliferation and promotes apoptosis in mouse embryonic palatal mesenchymal cells through Wnt/β-catenin signaling pathway.

J Physiol Biochem. 2013 Dec;69(4):855-63.

77.Ni Z, Dai X, Wang B, Ding W, Cheng P, Xu L, Lian J, He F.
Natural Bcl-2 inhibitor (-)- gossypol induces protective autophagy via reactive oxygen species-high mobility groupbox 1 pathway in Burkitt lymphoma.
Leuk Lymphoma. 2013 Oct;54(10):2263-8.
78.Jin M, Xiao R, Wang J, Liu X, Liu Y, Xue Z, Lv L, Zheng Y, Li Q.
Low concentrations of the recombinant toxin protein rLj-RGD3 suppress TNF-α-induced human renal carcinomacell invasion.
Acta Biochim Biophys Sin (Shanghai). 2013 May;45(5):377-82.
79.Zhang X, Ge YL, Zhang SP, Yan P, Tian RH.
Downregulation of KDR expression induces apoptosis in breast cancer cells.
Cell Mol Biol Lett. 2014 Dec;19(4):527-41.
80.Yang ZB, Tan B, Li TB, Lou Z, Jiang JL, Zhou YJ, Yang J, Luo XJ, Peng J.
Protective effect of vitexin compound B-1 against hypoxia/reoxygenation-induced injury in differentiated PC12 cells via NADPH oxidase inhibition.
Naunyn Schmiedebergs Arch Pharmacol. 2014 Sep;387(9):861-71. 
81.Zhao P, Liu J, Pan C, Pan Y.
NLRP3 inflammasome is required for apoptosis of Aggregatibacter actinomycetemcomitans -infected human osteoblastic MG63 cells.
Acta Histochem. 2014 Sep;116(7):1119-24. 
82.Wang W, Zhou X, Yang F, Sun H, Xu D, Andersson R, Chen B, Zhou M.
The effects of 5-Fluorouracil on severe acute pancreatitis-inducing apoptosis of macrophages.
Pancreas. 2014 May;43(4):660-3. 
83.Du J, Li XH, Zhang W, Yang YM, Wu YH, Li WQ, Peng J, Li YJ.
Involvement of glutamate-cystine/glutamate transporter system in aspirin-induced acute gastric mucosa injury.
Biochem Biophys Res Commun. 2014 Jul;450(1):135-41. 
84.Yao K, Xing H, Yang W, Liao A, Wu B, Li Y, Zhang R, Liu Z.
Knockdown of RLIP76 expression by RNA interference inhibits proliferation, enhances apoptosis, and increases chemosensitivity to daunorubicin in U937 leukemia cells.
Tumour Biol. 2014 Aug;35(8):8023-31.
85.Yang ZY, Kan JT, Cheng ZY, Wang XL, Zhu YZ, Guo W.
Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway.
Cytotechnology. 2014 Jan;66(1):51-61. 
86.Wang Y, Cai Y, Ji J, Liu Z, Zhao C, Zhao Y, Wei T, Shen X, Zhang X, Li X, Liang G.
Discovery and identification of new non-ATP competitive FGFR1 inhibitors with therapeutic potential on non-small-cell lung cancer.
Cancer Lett. 2014 Mar;344(1):82-9.
87.He Q, Sun J, Wang Q, Wang W, He B.
Neuroprotective effects of ginsenoside Rg1 against oxygen-glucose deprivation in cultured hippocampal neurons.
J Chin Med Assoc. 2014 Mar;77(3):142-9. 
88.Lin M, Li L, Zhang Y, Zheng L, Xu M, Rong R, Zhu T.
Baicalin ameliorates H2O2 induced cytotoxicity in HK-2 cells through the inhibition of ER stress and the activation of Nrf2 signaling.
Int J Mol Sci. 2014 Jul;15(7):12507-22. .
89.Li W, Sun H, Deng Y, Zhang A, Chen H.
The heterogeneity of the rDNA-ITS sequence and its phylogeny in Rhizoctonia cerealis, the cause of sharp eyespot in wheat.
Curr Genet. 2014 Feb;60(1):1-9. 
90.Liu T, Zhang J, Sun D, Sui W, Zhang Y, Li D, Chen Z, Gao H.
Comparative study of corneal endothelial cell damage after femtosecond laser assisted deep stromal dissection.
Biomed Res Int. 2014;2014:731565. 
91. Zhang B, Xie QY, Quan Y, Pan XM, Liao DF.
Reactive oxygen species induce cell death via Akt signaling in rat osteoblast-like cell line ROS 17/2.8.
Toxicol Ind Health. 2015 Dec;31(12):1236-42.
92. Xia B, Liu H, Xie J, Wu R, Li Y.
Akt enhances nerve growth factor-induced axon growth via activating the Nrf2/ARE pathway.
Int J Mol Med. 2015 Nov;36(5):1426-32.
93. Bounda GA, Zhou W, Wang DD, Yu F.
Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis throughMitochondria-Mediated Pathway.
Evid Based Complement Alternat Med. 2015;2015:329831.
94. Wang L, Song G, Liu M, Chen B, Chen Y, Shen Y, Zhu J, Zhou X.
MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation throughthe Notchsignaling pathway.
Int J Mol Med. 2015 Nov;37(1):47-55.
95. Dai JQ, Huang YG, He AN.
Dihydromethysticin kavalactone induces apoptosis in osteosarcoma cells through modulation of PI3K/Aktpathway, disruption of mitochondrial membrane potential and inducing cell cycle arrest.
Int J Clin Exp Pathol. 2015 May;8(5):4356-66.
96. Chenghui Shen, Yuemei Han, Bailiang Wang, Junmei Tang, Hao Chen and Quankui Lin.
Ocular biocompatibility evaluation of POSS nanomaterials for biomedical material applications.
RSC Adv. 2015 May; 8(5): 4356–66.
97. Jin H, Zhang X, Su J, Teng Y, Ren H, Yang L.
RNA interference mediated knockdown of translationally controlled tumor protein induces apoptosis, and inhibits growth and invasion in glioma cells.
Mol Med Rep. 2015 Nov;12(5):6617-25.
98. Wu J, Wei T, Tang Q, Weng B, Li W, Jiang X, Ding T, Li X, Liang G, Cai Y, Ji J.
Discovery and anti-cancer evaluation of two novel non-ATP-competitive FGFR1 inhibitors in non-small-cell lungcancer.
BMC Cancer. 2015 Apr;15:276.
99. Wang H, Sun R, Gu M, Li S, Zhang B, Chi Z, Hao L.
shRNA-Mediated Silencing of Y-Box Binding Protein-1 (YB-1) Suppresses Growth of Neuroblastoma Cell SH-SY5YIn Vitro and In Vivo.
PLoS One. 2015 May;10(5):e0127224.
100. Fan Y, Patima A, Chen Y, Zeng F, He W, Luo L, Jie Y, Zhu Y, Zhang L, Lei J, Xie X, Zhang H.
Cytotoxic effects ofβ-carboline alkaloids on human gastric cancer SGC-7901 cells.
Int J Clin Exp Med. 2015 Aug;8(8):12977-82.
101. Wang L, He HS, Yu HL, Zeng Y, Han H, He N, Liu ZG, Wang ZY, Xu SJ, Xiong M.
Sclareol,a plant diterpene, exhibits potent antiproliferative effects via the induction of apoptosis andmitochondrial membrane potential loss in osteosarcoma cancer cells.
Mol Med Rep. 2015 Jun;11(6):4273-8.
102. Wang Y, Liu W, He X, Fei Z.
Hispidulin enhances the anti-tumor effects of temozolomide in glioblastoma by activating AMPK.
Cell Biochem Biophys. 2015 Mar;71(2):701-6.
103. Ren H, Shao X, Zeng L, Wang F, Huang DN, Hou G.
Matrix metalloproteinase-mediation of tumor targeting human recombinant tumor necrosis factor-α fusion protein.
Mol Med Rep. 2015 Aug;12(2):2035-42.
104. Qin J, Teng J, Zhu Z, Chen J, Huang WJ.
Genistein induces activation ofthe mitochondrial apoptosispathway by inhibiting phosphorylation of Akt incolorectal cancer cells.
Pharm Biol. 2015 Apr;16:74-9.
105. Sui CG, Meng FD, Li Y, Jiang YH.
Antiproliferative activity of rosamultic acid is associated with induction of apoptosis, cell cycle arrest, inhibition ofcell migration and caspase activation in human gastric cancer (SGC-7901) cells.
Phytomedicine. 2015 Aug 15;22(9):796-806.
106. An F, Wang S, Tian Q, Zhu D.
Effects of orientin and vitexin from Trollius chinensis on the growth and apoptosis of esophageal cancer EC-109 cells.
Oncology Letters, 2015 Aug;10(4): 2627-33.
107. Zhou Y, Liu QH, Liu CL, Lin L.
Calycosin induces apoptosis in human ovarian cancer SKOV3 cells by activating caspases and Bcl-2 familyproteins.
Tumour Biol. 2015 Jul;36(7):5333-9.
108. Zhang QQ, Wang WJ, Li J, Yang N, Chen G, Wang Z, Liang ZQ.
Cathepsin L suppression increases the radiosensitivity of human glioma U251 cells via G2/M cell cycle arrest andDNA damage.
Acta Pharmacol Sin. 2015 Sep;36(9):1113-25.
109. Gang Li , Longfeng Sun , Zhongyi Mu, Yan Huang, Cheng Fu, Bin Hu.
Ectopic WWOX Expression Inhibits Growth of 5637 Bladder Cancer Cell In Vitro and In Vivo.
Cell Biochemistry and Biophysics November. 2015 Nov,73(2):417-25.
110. Wang S, Li H, Qian Z, Song X, Zhang Z, Zuo H, Xu X, Weng S, He J, Li C.
Identification and functional characterization ofthe TAB2 gene from Litopenaeus vannamei.
Fish Shellfish Immunol. 2015 Oct;46(2):206-16.
111. Li C, Li H, Wang S, Song X, Zhang Z, Qian Z, Zuo H, Xu X, Weng S, He J.
The c-Fos and c-Jun from Litopenaeus vannamei play opposite roles in Vibrio parahaemolyticus and white spotsyndrome virus infection.
Dev Comp Immunol. 2015 Sep;52(1):26-36.
112.Chen Z, Xue J, Shen T, Ba G, Yu D, Fu Q. Curcumin alleviates glucocorticoid-induced osteoporosis by protecting osteoblasts from apoptosis in vivo and invitro.
Clin Exp Pharmacol Physiol. 2016 Feb;43(2):268-76.
113.Gao X, Wang J, Bai W, Ji W, Wang L.
NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation ofJNK signaling pathway.
Oncol Rep. 2016 Jun;35(6):3313-20.
114.Huang Z, Huang X, Jiang D, Zhang Y, Huang B, Luo G.
Dihydroartemisinin inhibits cell proliferation by induced G1 arrest and apoptosis in humannasopharyngealcarcinoma cells.
J Cancer Res Ther. 2016 Jan-Mar;12(1):244-7.
115.Yao J, Jiao R, Liu C, Zhang Y, Yu W, Lu Y, Tan R.
Assessment of the Cytotoxic and Apoptotic Eἀects of Chaetominine in a Human Leukemia Cell Line.
Biomol Ther (Seoul). 2016 Mar 1;24(2):147-55.
116.Shi B, Li Y, Wang X, Yang Y, Li D, Liu X, Yang X.
Silencing of hypoxia inducible factor-1α by RNA interference inhibits growth of SK-NEP-1 Wilms tumourcells in vitro, and suppresses tumourigenesis and angiogenesis in vivo.
Clin Exp Pharmacol Physiol. 2016 Jun;43(6):626-33.
117.Yao J, Wei X, Lu Y.
Chaetominine reduces MRP1-mediated drug resistance via inhibiting PI3K/Akt/Nrf2 signaling pathway inK562/Adr human leukemia cells.
Biochem Biophys Res Commun. 2016 May 13;473(4):867-73.
118.Chen Z, Xue J, Shen T, Ba G, Yu D, Fu Q.
b Curcumin alleviates glucocorticoid-induced osteoporosis by protecting osteoblasts from apoptosis invivo and in vitro.
Clin Exp Pharmacol Physiol. 2016 Feb;43(2):268-76.
119.Liu X, Gong W, Xu YQ, Zhu BL.
Dimethylacetamide-induced Hepatic Injury in Vitro: Mechanism and Potential Preventive Strategy.
Biomed Environ Sci. 2016 Feb;29(2):153-7.
120.Xu H, Jin L, Chen Y, Li J.
Downregulation of microRNA-429 protects cardiomyocytes against hypoxia-induced apoptosis byincreasing Notch1 expression.
Int J Mol Med. 2016 Jun;37(6):1677-85.
121.Xie H, Yang J, Xing W, Dong Y, Ren H.
Nuclear EGFRvIII resists hypoxic microenvironment induced apoptosis via recruiting ERK1/2 nucleartranslocation.
Biochem Biophys Res Commun. 2016 Feb 5;470(2):466-72.
122.Guan HP, Sun JZ, Feng XL, Chen JS, Chen FJ, Cheng XF, Liu XW, Ni B.
Effects of RNA interference-mediated knockdown of livin and survivin using monomethoxypolyethyleneglycol-chitosan nanoparticles in MG-63 osteosarcoma cells.
Mol Med Rep. 2016 Feb;13(2):1821-6.
123.Feng J, Gou J, Jia J, Yi T, Cui T, Li Z.
Verteporfin, a suppressor of YAP-TEAD complex, presents promising antitumor properties on ovariancancer.
Onco Targets Ther. 2016 Aug 29;9:5371-81.
124.Song G, Shen Y, Ruan Z, Li X, Chen Y, Yuan W, Ding X, Zhu L, Qian L.
LncRNA-uc.167 influences cell proliferation, apoptosis and differentiation of P19 cells by regulatingMef2c.
Gene. 2016 Sep 15;590(1):97-108.
125.Wang L, Song G, Liu M, Chen B, Chen Y, Shen Y, Zhu J, Zhou X.
MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through theNotch signaling pathway.
Int J Mol Med. 2016 Jan;37(1):47-55.
126.Sui X, Li XH, Duan MH, Jia AL, Wang Y, Liu D, Li YP, Qiu ZD.
Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate.
Biomed Pharmacother. 2016 Jul;81:176-81.
127.Zhang YN, Duan XG, Zhang WH, Wu AL, Yang HH, Wu DM, Wei YQ, Chen XC.
Antitumor activity of pluripotent cell-engineered vaccines and their potential to treat lung cancer inrelation to different levels of irradiation.
Onco Targets Ther. 2016 Mar 11;9:1425-36.
128.Li A, Song J, Lai Q, Liu B, Wang H, Xu Y, Feng X, Sun X, Du Z .
Hypermethylation of ATP-binding cassette B1 (ABCB1) multidrug resistance 1 (MDR1) is associated withcisplatin resistance in the A549 lung adenocarcinoma cell line.
Int J Exp Pathol. 2016 Dec 20. doi: 10.1111/iep.12212. [Epub ahead of print]
129.Qin J, Teng J, Zhu Z, Chen J, Huang WJ.
Genistein induces activation of the mitochondrial apoptosis pathway by inhibiting phosphorylation ofAkt in colorectal cancer cells.
Pharm Biol. 2016;54(1):74-9.
130.Wang S, Qian Z, Li H, Lu K, Xu X, Weng S, He J, Li C.
Identification and characterization of MKK7 as an upstream activator of JNK in Litopenaeus vannamei.
Fish Shellfish Immunol. 2016 Jan;48:285-94.
131.Huang K, Li Y, Tao S, Wei G, Huang Y, Chen D, Wu C.
Purification, Characterization and Biological Activity of Polysaccharides from Dendrobium officinale.
Molecules. 2016 May 30;21(6).pii: E701.
132. Chen X, Lv Q, Liu Y, Deng W.
Effect of Food Additive Citric Acid on The Growth of Human Esophageal Carcinoma Cell Line EC109.
Cell J. 2017 Winter;18(4):493-502.
133.Fang Y, Xu Z, Shi Y, Pei F, Yang W, Ma N, Kimatu BM, Liu K, Qiu W, Hu Q.
Protection mechanism of Se-containing protein hydrolysates from Se-enriched rice on Pb2+-inducedapoptosis in PC12 and RAW264.7 cells.
Food Chem. 2017 Mar 15;219:391-398.