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 乳酸脱氢酶细胞毒性检测试剂盒

  产品编号C0017
  产品包装: 500次
  产品价格: 516.00元
产品编号 产品名称 产品包装 产品价格
C0017 乳酸脱氢酶细胞毒性检测试剂盒 500次 516.00元

      碧云天的乳酸脱氢酶细胞毒性检测试剂盒(LDH Cytotoxicity Assay Kit),也称乳酸脱氢酶检测试剂盒(LDH Assay Kit)或乳酸脱氢酶释放检测试剂盒(LDH Release Assay Kit),是一种基于diaphorase催化的INT显色反应,通过比色法检测细胞毒性时释放的乳酸脱氢酶活性或检测其它样品中的乳酸脱氢酶活性的试剂盒。
      本试剂盒可以用于常规的乳酸脱氢酶活性的检测,更常用于以LDH释放为指标的细胞毒性检测。同时,基于细胞总乳酸脱氢酶活性的检测,本试剂盒也可以用于检测细胞增殖和细胞毒性检测。
      细胞凋亡或坏死而造成的细胞膜结构的破坏会导致细胞浆内的酶释放到培养液里,其中包括酶活性较为稳定的乳酸脱氢酶(lactate dehydrogenase, LDH)。通过检测从质膜破裂的细胞中释放到培养液中的LDH的活性,就可以实现对细胞毒性的定量分析。LDH释放被看做细胞膜完整性的重要指标,并被广泛用于细胞毒性检测。LDH释放被认为是以前使用放射性的51Cr标记细胞,随后通过51Cr释放进行细胞膜完整性检测的安全有效的替代方法。
      本试剂盒的基本原理是,在乳酸脱氢酶的作用下,NAD+被还原生成NADH,NADH和INT (2-p-iodophenyl-3-nitrophenyl tetrazolium chloride)被硫辛酰胺脱氢酶(diaphorase)催化反应生成NAD+和强生色物甲臜(formazan),在490nm波长下产生吸收峰,从而可以通过比色来定量乳酸脱氢酶的活性。吸光度与乳酸脱氢酶活性成线性正相关。该酶联反应原理的示意图如下:

      可检测细胞培养液、细胞裂解液等样品中乳酸脱氢酶的活性。一个试剂盒可进行500次检测。
包装清单:

产品编号 产品名称 包装
C0017-1 LDH释放试剂 7.5ml
C0017-2 乳酸溶液 10ml
C0017-3 酶溶液 5ml×2
C0017-4 INT溶液(10X) 1ml
C0017-5 INT稀释液 10ml
说明书 1份

保存条件:
      -20℃保存,一年有效。C0017-3需注意避免反复冻融。C0017-4需避光保存。试剂盒解冻后可以短期4℃存放,2-3天内有效。
注意事项:
      冷冻会使样品中部分乳酸脱氢酶失活,4℃可放置2-3天。建议样品准备好后尽量当天完成测定。
      如果检测细胞培养液中的乳酸脱氢酶,由于血清含有乳酸脱氢酶,建议血清的使用浓度不要超过1%,并最好使用热灭活血清。如果一定需要使用10%血清,在检测时一定要设置没有细胞,但加入了相同体积培养液的对照孔,以用于消除背景。
      细胞过度生长、密度过高、离心速度过大、培养箱内外温差过大,都会造成细胞释放乳酸脱氢酶增加。
      如果希望进行乳酸脱氢酶活性的绝对定量,用户需自备乳酸脱氢酶标准品。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.样品的准备:
方法一:LDH释放检测
a.根据细胞的大小和生长速度将适量细胞接种到96孔细胞培养板中,使待检测时细胞密度不超过80-90%满。
b.吸去培养液,用PBS液洗涤一次。换新鲜培养液(推荐使用含1%血清的低血清培养液或适当的无血清培养液),将各培养孔分成如下几组:包括无细胞的培养 液孔(背景空白对照孔),未经药物处理的对照细胞孔(样品对照孔),未经药物处理的用于后续裂解的细胞孔(样品最大酶活性对照孔),以及药物处理的细胞 孔(药物处理样品孔),并做好标记。按照实验需要给予适当药物处理(如加入0-10μl左右特定的药物刺激,可设置不同浓度,不同处理时间,对照孔中需加 入适当的药物溶剂对照),继续按常规培养。到预定的检测时间点前1小时,从细胞培养箱里取出细胞培养板,在“样品最大酶活性对照孔”中加入试剂盒提供 的LDH释放试剂,加入量为原有培养液体积的10%。加入LDH释放试剂后,反复吹打数次混匀,然后继续在细胞培养箱中孵育。
c.到达预定时间后,将细胞培养板用多孔板离心机400g离心5min。分别取各孔的上清液120μl,加入到一新的96孔板相应孔中,随即进行样品测定。
方法二:细胞内总LDH的检测
a.细胞毒性检测:根据细胞的大小和生长速度将适量细胞接种到96孔细胞培养孔板中,使待检测时细胞密度不超过80-90%满。加入不同药物进行处理,并设 置适当对照。药物刺激完毕后,将细胞培养板用多孔板离心机400g离心5min。尽量吸除上清,加入150μl用PBS稀释了10倍的试剂盒提供的LDH释放试剂(10 体积PBS中加入1体积LDH释放试剂并混匀),适当摇晃培养板混匀,然后继续在细胞培养箱中孵育1小时。随后将细胞培养板用多孔板离心机400g离心5min。分 别取各孔的上清液120μl,加入到一新的96孔板相应孔中,随即进行样品测定。
b.细胞增殖检测:根据细胞的大小和生长速度将适量细胞接种到96孔细胞培养孔板中,使促进细胞增殖的药物刺激后细胞不超过80-90%满为宜。使用不同的 药物刺激细胞,并设置适当对照。药物刺激完毕后,将细胞培养板用多孔板离心机400g离心5min。尽量吸除上清,加入150μl用PBS稀释了10倍的试剂盒提供 的LDH释放试剂(10体积PBS中加入1体积LDH释放试剂并混匀),适当摇晃混匀胞,然后继续在细培养箱中孵育1小时。随后将细胞培养板用多孔板离心机400g离 心5min。分别取各孔上清液120μl,加入到一新的96孔板相应孔中,随即进行样品测定。
注:LDH释放检测更加常用一些,细胞内总LDH检测通常可以使用MTT、WST-1或CCK-8等方法替代。
2. 试剂盒的准备工作:
a.INT溶液(1X)的配置:根据所需的INT溶液(1X)的量,取适量INT溶液(10X)用INT稀释液稀释至1X。例如,取20μl INT溶液(10X),加入180μl INT稀释液 ,混匀后即配置为200μl INT溶液(1X)。INT溶液(1X)宜现配现用,配置后4℃保存可于当天使用,不宜配置后冻存。
b.LDH检测工作液的配制: 根据待测定的样品数(含对照),参考下表在临检测前新鲜配制适量的检测工作液。
注意:LDH检测工作液必须现配现用,配制和使用过程中均要注意适当避光。

检测次数 1次 10次 20次 50次
乳酸溶液 20μl 200μl 400μl 1ml
INT溶液(1X) 20μl 200μl 400μl 1ml
酶溶液 20μl 200μl 400μl 1ml
总体积 60μl 600μl 1.2 ml 3ml

c.(选做)如果希望进行LDH酶活性的绝对定量,需自备LDH标准品,并新鲜配制不同浓度LDH标准品,如10mU/ml、5mU/ml、2.5mU/ml、1.25mU/ml、 0.65mU/ml、0 mU/ml。
3. 样品测定:
a.各孔分别加入60μl LDH检测工作液。
b.混匀,室温(约25℃) 避光孵育30min(可用铝箔包裹后置于水平摇床或侧摆摇床上缓慢摇动)。然后在490nm处测定吸光度。使用600nm或大于600nm的任一 波长作为参考波长进行双波长测定。
c.计算(测得的各组吸光度均应减去背景空白对照孔吸光度)
d.细胞毒性或死亡率(%)=(处理样品吸光度-样品对照孔吸光度) / (细胞最大酶活性的吸光度-样品对照孔吸光度)×100
e.可绘制细胞毒性曲线:纵座标为实际吸光度,横坐标为药物浓度;据此可计算该药物作用特定时间的半致死剂量LD50。

附录1:
可同时测定一已知浓度的LDH酶标准品对应的吸光度值,参考以下公式粗略计算出样品中LDH酶活力:
待测样品中LDH活力单位(mU/ml)=
(样品孔吸光度-背景空白对照孔吸光度) / (标准管吸光度-标准空白管吸光度)×标准品浓度(mU/ml);
根据计算结果可以比较不同样品处理组间有无统计学差异等。

附录2:
若需准确计算出LDH酶活性的绝对活性,可通过一系列LDH标准品及相应测得的吸光度值绘制标准曲线,通过标准曲线相应公式计算出样品中LDH的酶活性。
各孔数值减去空白对照后,以检测的吸光度(OD490)为纵坐标,LDH酶活力(mU)为横坐标,绘制LDH标准曲线。同时计算出该趋势线的公式。

A490nm=k×LDH酶活力单位(mU)+b,通过Excel等软件计算出趋势线的斜率k和截距b。
根据上述公式计算样品中LDH活力。
样品实际吸光度(OD490)=样品孔测得的吸光度-背景空白对照孔吸光度
检测体系中LDH酶活力单位(mU)=(OD490-b)/k
样品中LDH酶活力(mU/ml)=检测体系中LDH酶活力单位(mU )/检测样品体积

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28.Lu C, Liu X, Liu C, Wang J, Li C, Liu Q, Li Y, Li S, Sun S, Yan J, Shao J.
Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and TopoisomeraseII Inhibition in Human Fetal Liver Hematopoietic Stem Cells.
Toxicol Sci. 2015 Oct;147(2):588-606.
29.Zhang P, Huang C, Wang W, Wang M.
Early changes in staurosporine-induced differentiated RGC-5 cells indicate cellular injury response to nonlethalblue light exposure.
Photochem Photobiol Sci. 2015 Jun;14(6):1093-9.
30.Yan YX, Zhao JX, Han S, Zhou NJ, Jia ZQ, Yao SJ, Cao CL, Wang YL, Xu YN, Zhao J, Yan YL, Cui HX.
Tetramethylpyrazine induces SH-SY5Y cell differentiation toward the neuronal phenotype through activation of the PI3K/Akt/Sp1/TopoIIβ pathway.
Eur J Cell Biol. 2015 Dec;94(12):626-41.
31.Liang ZE, Yi YJ, Guo YT, Wang RC, Hu QL, Xiong XY.
Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides fromGanoderma lucidum.
Mol Med Rep. 2015 Nov;12(5):7629-36.
32.Liu C, Mei W, Tang J, Yuan Q, Huang L, Lu M, Wu L, Peng Z, Meng J, Yang H, Shen H, Lv B, Hu G, Tao L.
Mefunidone attenuates tubulointerstitial fibrosis in a rat model of unilateral ureteral obstruction.
PLoS One. 2015 Jun 4;10(6):e0129283.
33.Li F, Tan YS, Chen HL, Yan Y, Zhai KF, Li DP, Kou JP, Yu BY.
Identification of schisandrin asa vascular endothelium protective component in YiQiFuMai Powder Injection usingHUVECs binding and HPLC-DAD-Q-TOF-MS/MS analysis.
J Pharmacol Sci. 2015 Sep;129(1):1-8.
34.Bounda GA, Zhou W, Wang DD, Yu F.
Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis throughMitochondria-Mediated Pathway.
Evid Based Complement Alternat Med. 2015;2015:329831.
35.Tao Z, Zhao H, Wang R, Liu P, Yan F, Zhang C, Ji X, Luo Y.
Neuroprotective effect of microRNA-99a against focal cerebral ischemia-reperfusion injury in mice.
J Neurol Sci. 2015 Aug 15;355(1-2):113-9.
36.Liu C, Mei W, Tang J, Yuan Q, Huang L, Lu M, Wu L, Peng Z, Meng J, Yang H, Shen H, Lv B, Hu G, Tao L.
Mefunidone attenuates tubulointerstitial fibrosis in a rat model of unilateral ureteral obstruction.
PLoS One. 2015 Jun 4;10(6):e0129283.
37.Li F, Tan YS, Chen HL, Yan Y, Zhai KF, Li DP, Kou JP, Yu BY.
Identification of schisandrin asa vascular endothelium protective component in YiQiFuMai Powder Injection usingHUVECs binding and HPLC-DAD-Q-TOF-MS/MS analysis.
J Pharmacol Sci. 2015 Sep;129(1):1-8.
38.Bounda GA, Zhou W, Wang DD, Yu F.
Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis throughMitochondria-Mediated Pathway.
Evid Based Complement Alternat Med. 2015;2015:329831.
39.Tao Z, Zhao H, Wang R, Liu P, Yan F, Zhang C, Ji X, Luo Y.
Neuroprotective effect of microRNA-99a against focal cerebral ischemia-reperfusion injury in mice.
J Neurol Sci. 2015 Aug 15;355(1-2):113-9.
40.Yang L, Tseng YT, Suo G, Chen L, Yu J, Chiu WJ, Huang CC, Lin CH.
Photothermal therapeutic response of cancer cells to aptamer-gold nanoparticle-hybridized graphene oxideunder NIR illumination.
ACS Appl Mater Interfaces. 2015 Mar 11;7(9):5097-106.
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17β-estradiol protects against apoptosis induced by interleukin-1β in rat nucleus pulposus cells by down-regulating MMP-3 and MMP-13. 
Apoptosis.2015 Mar;20(3):348-57. 
42.Zhu D, Zhang J, Wu J, Li G, Yao W, Hao J, Sun J.
Paliperidone Protects SH-SY5Y Cells Against MK-801-Induced Neuronal Damage Through Inhibition of Ca2+ Influx and Regulation of SIRT1/miR-134 Signal Pathway.
Mol Neurobiol. 2016 May;53(4):2498–509. 
43.Liu YP, Dong FX, Chai X, Zhu S, Zhang BL, Gao DS. 
Role of Autophagy in Capsaicin-Induced Apoptosis in U251 Glioma Cells.
Cell Mol Neurobiol. 2016 Jul;36(5):737–43.
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Progesterone amplifies oxidativestress signal and promotes NO production via H2O2in mouse kidney arterialendothelial cells.
J Steroid Biochem Mol Biol. 2016 Jan;155(Pt A):104-11.
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Nano zerovalent iron particles induce pulmonary and cardiovascular toxicity in an in vitro human co-culture model. 
Nanotoxicology. 2016 Sep;10(7):881-90.
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MLIF Alleviates SH-SY5Y Neuroblastoma Injury Induced by Oxygen-Glucose Deprivation by TargetingEukaryotic Translation Elongation Factor 1A2.
PLoS One. 2016 Feb 26;11(2):e0149965. 
47.Du J, Cheng X, Shen L, Tan Z, Luo J, Wu X, Liu C, Yang Q, Jiang Y, Tang G, Li X, Zhang S, Zhu L.
Methylation of miR-145a-5p promoter mediates adipocytes differentiation.
Biochem Biophys Res Commun. 2016 Jun 17;475(1):140-8.
48.Xie Y, Liu D, Cai C, Chen X, Zhou Y, Wu L, Sun Y, Dai H, Kong X, Liu P.
Size-dependent cytotoxicity of Fe3O4 nanoparticles induced by biphasic regulation of oxidative stress indifferent human hepatoma cells.
Int J Nanomedicine. 2016 Jul 29;11:3557-70.
49.Ye W, Zhong Z, Zhu S, Zheng S, Xiao J, Song S, Yu H, Wu Q, Lin Z, Chen J.
Advanced oxidation protein products induce catabolic effect through oxidant-dependent activation of NF-κ B pathway in human chondrocyte.
Int Immunopharmacol. 2016 Oct;39:149-57.
50.Yang XY, Shi T, Du G, Liu W, Yin XF, Sun X, Pan Y, He QY.
iTRAQ-Based Proteomics Revealed the Bactericidal Mechanism of Sodium New Houttuyfonate againstStreptococcus pneumoniae. 
J Agric Food Chem. 2016 Aug 17;64(32):6375-82.
51.Yuan Z, Matias FB, Yi JE, Wu J.
T-2 toxin-induced cytotoxicity and damage on TM3 Leydig cells.
Comp Biochem Physiol C Toxicol Pharmacol. 2016 Mar-Apr;181-182:47-54.
52.Wu WB, Menon R, Xu YY, Zhao JR, Wang YL, Liu Y, Zhang HJ.
Downregulation of peroxiredoxin-3 by hydrophobic bile acid induces mitochondrial dysfunction andcellular senescence in human trophoblasts.
Sci Rep. 2016 Dec 13;6:38946.
53.Zheng M, Liu C, Fan Y, Shi D, Zhang Y .
Protective Effects of Paeoniflorin Against MPP(+)-induced Neurotoxicity in PC12 Cells.
Neurochem Res. 2016 Jun;41(6):1323-34.
54.Chen G, Zhao Q, Zhu F, Chen R, Jin Y, Liu C, Pan X, Jin S, Wu W, Cheng Z.
Oligoribonuclease is required for the type III secretion system and pathogenesis of Pseudomonas aeruginosa.
Microbiol Res. 2016 Jul-Aug;188-189:90-6.
55.Peng X, Gan J, Wang Q, Shi Z, Xia X.
3-Monochloro-,2-propanediol (3-MCPD) induces apoptosis via mitochondrial oxidative phosphorylationsystem impairment and the caspase cascade pathway.
Toxicology. 2016 Nov 30;372:1-11.
56.Yu M, Chen R, Jia Z, Chen J, Lou J, Tang S, Zhang X.
MWCNTs Induce ROS Generation, ERK Phosphorylation, and SOD-2 Expression in Human MesothelialCells.
Int J Toxicol. 2016 Jan-Feb;35(1):17-26.
57.Su M, Huang J, Liu S, Xiao Y, Qin X, Liu J, Pi C, Luo T, Li J, Chen X, Luo Z.
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Sci Rep. 2016 Jun 24;6:28139.
58.Wang T, Chen X, Long X, Liu Z, Yan S.
Copper Nanoparticles and Copper Sulphate Induced Cytotoxicity in Hepatocyte Primary Cultures ofEpinephelus coioides.
PLoS One. 2016 Feb 18;11(2):e0149484.
59.Wei Z, Hermosilla C, Taubert A, He X, Wang X, Gong P, Li J, Yang Z, Zhang X.
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Front Immunol. 2016 Oct 31;7:436.
60.Wang XD, Li CY, Jiang MM, Li D, Wen P, Song X, Chen JD, Guo LX, Hu XP, Li GQ, Zhang J, Wang CH, He ZD.
Induction of apoptosis in human leukemia cells through an intrinsic pathway by cathachunine, a uniquealkaloid isolated from Catharanthus roseus.
Phytomedicine. 2016 Jun 1;23(6):641-53.
61.Zhang LW, Cong X, Zhang Y, Wei T, Su YC, Serrão AC, Brito AR Jr, Yu GY, Hua H, Wu LL.
Interleukin-17 Impairs Salivary Tight Junction Integrity in Sjögren's Syndrome. J Dent Res. 2016 Jul;95(7):784-92.
62.Li N, Shang L, Wang SC, Liao LS, Chen D, Huang JF, Xiong K.
The Toxic Effect of ALLN on Primary Rat Retinal Neurons.
Neurotox Res. 2016 Oct;30(3):392-406.
63.Niu Y, Sun W, Lu JJ, Ma DL, Leung CH, Pei L, Chen X.
PTEN Activation by DNA Damage Induces Protective Autophagy in Response to Cucurbitacin B inHepatocellular Carcinoma Cells.
Oxid Med Cell Longev. 2016;2016:4313204.
64.Wang S, Li X, Niu Y, Liu Y, Zhu Y, Lu X, Fan X, Zhang X, Wang Y.
Identification and screening of chemical constituents with hepatoprotective effects from three traditionalChinese medicines for treating jaundice.
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65.Xu Y, Zhi F, Shao N, Wang R, Yang Y, Xia Y.
Cytoprotection against Hypoxic and/or MPP⁺ Injury: Effect of δ-Opioid Receptor Activation on Caspase 3.
Int J Mol Sci. 2016 Aug 9;17(8).pii: E1179.
66.Zhou J, Ling J, Song J, Wang Y, Feng B, Ping F.
Interleukin 10 protects primary melanocyte by activation of Stat-3 and PI3K/Akt/NF-κB signalingpathways.
Cytokine. 2016 Jul;83:275-81.
67.Han S, Lu Q, Wang N. 
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Mol Med Rep. 2016 Apr;13(4):3121-6.
68.Xiong K, Liao H, Long L, Ding Y, Huang J, Yan J.
Necroptosis contributes to methamphetamine-induced cytotoxicity in rat cortical neurons.
Toxicol In Vitro. 2016 Sep;35:163-8.
69.Li M, Long Y, Liu Y, Liu Y, Chen R, Shi J, Zhang L, Jin Y, Yang L, Bai F, Jin S, Cheng Z, Wu W.
HigB of Pseudomonas aeruginosa Enhances Killing of Phagocytes by Up-Regulating the Type IIISecretion System in Ciprofloxacin Induced Persister Cells.
Front Cell Infect Microbiol. 2016 Oct 14;6:125.
70.Weng Y, Chen F, Liu Y, Zhao Q, Chen R, Pan X, Liu C, Cheng Z, Jin S, Jin Y, Wu W.
Pseudomonas aeruginosa Enolase Influences Bacterial Tolerance to Oxidative Stresses and Virulence.
Front Microbiol. 2016 Dec 15;7:1999.
71.Lu Y, Zhang TF, Shi Y, Zhou HW, Chen Q, Wei BY, Wang X, Yang TX.
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, inducesnecrosis in leukemia cells.
Sci Rep. 2016 Feb 10;6:20823.
72.Yan H, Li Y, Peng X, Huang D, Gui L, Huang B.
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Mol Med Rep. 2016 May;13(5):4393-9.
73.Jiang X, Lin H, Jiang D, Xu G, Fang X, He L, Xu M, Tang B, Wang Z, Cui D, Chen F, Geng H.
Co-delivery of VEGF and bFGF via a PLGA nanoparticle-modified BAM for effective contracture inhibitionof regenerated bladder tissue in rabbits.
Sci Rep. 2016 Feb 8;6:20784.
74.Zhang J, Zheng L, Yuan X, Liu C, Yuan Q, Xie F, Qiu S, Peng Z, Tang Y, Meng J, Qin J, Hu G, Tao L.
Mefunidone ameliorates renal inflammation and tubulointerstitial fibrosis via suppression of IKKβphosphorylation.
Int J Biochem Cell Biol. 2016 Nov;80:109-118.
75.Guo HL, Hassan HM, Ding PP, Wang SJ, Chen X, Wang T, Sun LX, Zhang LY, Jiang ZZ.
Pyrazinamide-induced hepatotoxicity is alleviated by 4-PBA via inhibition of the PERK-eIF2α-ATF4-CHOPpathway.
Toxicology. 2017 Mar 1;378:65-75.
76.Wang C, Yang J, Zhu L, Yan L, Lu D, Zhang Q, Zhao M, Li Z.
Never deem lightly the "less harmful" low-molecular-weight PAH, NPAH, and OPAH - Disturbance of theimmune response at real environmental levels.
Chemosphere. 2017 Feb;168:568-577.
77.Guo HL, Hassan HM, Ding PP, Wang SJ, Chen X, Wang T, Sun LX, Zhang LY, Jiang ZZ.
Pyrazinamide-induced hepatotoxicity is alleviated by 4-PBA via inhibition ofthePERK-eIF2α-ATF4-CHOP pathway.
Toxicology. 2017 Mar 1;378:65-75.
78.Zhong Y, Jin C, Gan J, Wang X, Shi Z, Xia X, Peng X.
Apigenin attenuates patulin-induced apoptosis in HEK293 cells by modulating ROS-mediatedmitochondrial dysfunction and caspase signal pathway.
Toxicon. 2017 Oct;137:106-113.
79.Qiu M, Ke L, Zhang S, Zeng X, Fang Z, Liu J.
JS-K,a GST-activated nitric oxide donor prodrug, enhances chemo-sensitivity in renal carcinomacells and prevents cardiac myocytes toxicity induced by Doxorubicin.
Cancer Chemother Pharmacol. 2017 Aug;80(2):275-286.
80.Zhang L, Zhu Z, Tan Z, Luo H, Hu X, Li Y.
Docosahexaenoic acid induces glial cell-line derived neurotrophic factor release in C6 gliomacells: Implications of antidepressant effects for docosahexaenoic acid.
Biochem Biophys Res Commun. 2017 Sep 30;491(4):1112-1117.
81.Cui J, Wang J, Zheng M, Gou D, Liu C, Zhou Y.
Ginsenoside Rg2 protects PC12 cells against β-amyloid25-35-induced apoptosis via the phosphoinositide 3-kinase/Akt pathway.
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82.Jing R, Zhou Z, Kuang F, Huang L, Li C.
microRNA-99a Reduces Lipopolysaccharide-Induced Oxidative Injury by Activating NotchPathway in H9c2 Cells.
Int Heart J. 2017 May 31;58(3):422-427.
83.Kang TS, Wang W, Zhong HJ, Dong ZZ, Huang Q, Mok SW, Leung CH, Wong VK, Ma DL.
An anti-prostate cancer benzofuran-conjugated iridium(III) complex asa dual inhibitor of STAT3 and NF-κB.
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84.Wang N, Zhang L, Lu Y, Zhang M, Zhang Z, Wang K, Lv J.
Down-regulation of microRNA-142-5p attenuates oxygen-glucose deprivation and reoxygenation-induced neuron injury through up-regulating Nrf2/ARE signaling pathway.
Biomed Pharmacother. 2017 May;89:1187-1195.
85.Zhou Z, Tang X, Chen H, Wang Y.
Comparative studies of saxitoxin (STX)-induced cytotoxicity in Neuro-2a and RTG-2 cell lines:An explanation with respect to changes in ROS.
Chemosphere. 2017 Oct 14;192:66-74.
86.Yin W, Song Y, Liu Q, Wu Y, He R.
Topical treatment of all-trans retinoic acid inhibits murine melanoma partly by promoting CD8+ T-cell immunity.
Immunology. 2017 Oct;152(2):287-297.
87.Liu YG, Chen JK, Zhang ZT, Ma XJ, Chen YC, Du XM, Liu H, Zong Y, Lu GC.
NLRP3 inflammasome activation mediates radiation-induced pyroptosis in bone marrow-derivedmacrophages.
Cell Death Dis. 2017 Feb 2;8(2):e2579.
88.Xu XH, Zhang LL, Wu GS, Chen X, Li T, Chen X, Wang YT, Lu JJ.
Solasodine Induces Apoptosis, Affects Autophagy, and Attenuates Metastasis in Ovarian CancerCells.
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89.Liu S, Liang B, Jia H, Jiao Y, Pang Z, Huang Y.
Evaluation of cell death pathways initiated by antitumor drugs melatonin and valproic acid in bladder cancer cells.
FEBS Open Bio. 2017 Apr 27;7(6):798-810.
90.Yan J, Lai CH, Lung SC, Chen C, Wang WC, Huang PI, Lin CH.
Industrial PM2.5 cause pulmonary adverse effect through RhoA/ROCK pathway.
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91.Chen C, Bu W, Ding H, Li Q, Wang D, Bi H, Guo D.
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92.Li J, Qiu M, Chen L, Liu L, Tan G, Liu J.
Resveratrol promotes regression of renal carcinoma cells via a renin-angiotensin systemsuppression-dependent mechanism.
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93.Ju L, Wu W, Yu M, Lou J, Wu H, Yin X, Jia Z, Xiao Y, Zhu L, Yang J.
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94.Mao Y, Wang J, Zhang M, Fan W, Tang Q, Xiong S, Tang X, Xu J, Wang L, Yang S, Liu S, Xu L, Chen Y, Xu L, Yin R, Zhu J.
A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signalingpathway.
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95.Deng X, Li M, Pan X, Zheng R, Liu C, Chen F, Liu X, Cheng Z, Jin S, Wu W.
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96.Lu G, Pan Y, Kayoumu A, Zhang L, Yin T, Tong Z, Li B, Xiao W, Ding Y, Li W.
Indomethacin inhabits the NLRP3 inflammasome pathway and protects severe acute pancreatitis in mice.
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97.Zhang XL, Yuan YH, Shao QH, Wang ZZ, Zhu CG, Shi JG, Ma KL, Yan X, Chen NH.
DJ-1 regulating PI3K-Nrf2 signaling plays a significant role in bibenzyl compound 20C-mediatedneuroprotection against rotenone-induced oxidative insult.
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98.Ju L, Wu W, Yin X, Xiao Y, Jia Z, Lou J, Yu M, Ying S, Chen T, Jiang Z, Li W, Chen J, Zhang X, Zhu L.
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FEBS Open Bio. 2017 Aug 30;7(10):1469-1479.
99.Li Y, Wu Z, Liu K, Qi P, Xu J, Wei J, Li B, Shao D, Shi Y, Qiu Y, Ma Z.
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100.Ren Y, Li S, Wu Z, Zhou C, Zhang D, Chen X.
The Influences of Bacillus subtilis onthe Virulence of Aeromonas hydrophila and Expression of luxS Gene of Both Bacteria Under Co-cultivation.
Curr Microbiol. 2017 Jun;74(6):718-724.
101.Xia L, Dai L, Zhu L, Hu W, Yang Q.
Proteomic Analysis of IPEC-J2 Cells in Response to Coinfection by Porcine TransmissibleGastroenteritis Virus and Enterotoxigenic Escherichia coli K88.
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102.Li Y, Liu M, Zuo Z, Liu J, Yu X, Guan Y, Zhan R, Han Q, Zhang J, Zhou R, Sun R, Tian Z, Zhang C.
TLR9 Regulates theNF-κB-NLRP3-IL-1β Pathway Negatively in Salmonella-Induced NKG2D-Mediated Intestinal Inflammation.
J Immunol. 2017 Jul 15;199(2):761-773.
103.WANG Jing, ZHOU Jun, MO Wei-Chuan, HE Ying-Ge, WEI Yan, HE Rong-Qiao, YI Fa-Ping.
Accumulation of Simulated Pathological Level of Formaldehyde Decreases Cell Viability and Adhesive Morphology in Neuronal Cells.
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104.Ying Sun, Qi Wang, Jianhua Chen, Lei Liu, Li Ding, Ming Shen, Jin Li, Baoshan Han and Yourong Duan.
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