SDS-PAGE凝胶配制试剂盒(P0012A)

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SDS-PAGE凝胶配制试剂盒

产品编号: P0012A

产品包装:可制30-50块胶

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可制30-50块胶

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价格: ¥ 163.00

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产品编号	产品名称	产品包装	产品价格
P0012A	SDS-PAGE凝胶配制试剂盒	可制30-50块胶	163.00元

碧云天生产的SDS-PAGE凝胶配制试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂，用户只需自备制胶器具和蒸馏水，即可配制PAGE胶(即聚丙烯酰胺凝胶)。
SDS-PAGE凝胶配制试剂盒不仅可用于配制SDS-PAGE凝胶，也可用于配制非变性(native)PAGE凝胶。
本试剂盒约可配制30-50块常规大小的PAGE胶。
包装清单：

产品编号	产品名称	包装
P0012A-1	30% Acr-Bis (29:1)	100ml
P0012A-2	1M Tris-HCl, pH8.8	100ml
P0012A-3	10% SDS	5ml
P0012A-4	凝胶聚合催化剂	0.5g
P0012A-5	TEMED	0.5ml
P0012A-6	1M Tris-HCl, pH6.8	15ml
—	说明书	1份

保存条件：
1M Tris-HCl,pH8.8、10% SDS、凝胶聚合催化剂和1M Tris-HCl,pH6.8室温保存。30% Acr-Bis(29:1)和TEMED 4℃避光保存。凝胶聚合催化剂加水配制成10%溶液后，分装成小管-20℃保存，通常半年内有效。
注意事项：
凝胶聚合催化剂配制成10%溶液后，应当-20℃保存。同时应尽量减少室温存放时间，以防失效。
TEMED易挥发，使用后请盖紧瓶盖。另外凝胶凝聚的速度和温度及光照关系密切，可通过适当调节凝胶聚合催化剂和TEMED的用量，控制在不同的室内环境下凝胶凝聚的速度。
TEMED易燃，有腐蚀性，操作时请小心，并注意有效防护以避免直接接触人体或腐蚀其他物品。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.10%凝胶聚合催化剂的配制：例如称取0.1g凝胶聚合催化剂，用水溶解，并定容至1ml，即为10%凝胶聚合催化剂。
2.根据目的蛋白的分子量大小选择合适的凝胶浓度，再按照下面的表格配制SDS-PAGE的分离胶(即下层胶)：
不同浓度的SDS-PAGE分离胶的最佳分离范围：

SDS-PAGE分离胶浓度	最佳分离范围
6%胶	50-150kD
8%胶	30-90kD
10%胶	20-80kD
12%胶	12-60kD
15%胶	10-40kD

成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
6%胶	5	10	15	20	30	50
蒸馏水	2.0	4.0	6.0	8.0	12.0	20.0
30%Acr-Bis(29:1)	1.0	2.0	3.0	4.0	6.0	10.0
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED	0.004	0.008	0.012	0.016	0.024	0.04
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
8%胶	5	10	15	20	30	50
蒸馏水	1.7	3.3	5	6.7	10.0	16.7
30%Acr-Bis(29:1)	1.3	2.7	4.0	5.3	8.0	13.3
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED	0.003	0.006	0.009	0.012	0.018	0.03
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
10%胶	5	10	15	20	30	50
蒸馏水	1.3	2.7	4.0	5.3	8.0	13.3
30%Acr-Bis(29:1)	1.7	3.3	5.0	6.7	10.0	16.7
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED	0.002	0.004	0.006	0.008	0.012	0.02
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
12%胶	5	10	15	20	30	50
蒸馏水	1.0	2.0	3.0	4.0	6.0	10.0
30%Acr-Bis(29:1)	2.0	4.0	6.0	8.0	12.0	20.0
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED	0.002	0.004	0.006	0.008	0.012	0.02
成分	配制不同体积SDS-PAGE分离胶所需各成分的体积(毫升)
15%胶	5	10	15	20	30	50
蒸馏水	0.5	1.0	1.5	2.0	3.0	5.0
30%Acr-Bis(29:1)	2.5	5.0	7.5	10.0	15.0	25.0
1M Tris, pH8.8	1.9	3.8	5.7	7.6	11.4	19.0
10%SDS	0.05	0.1	0.15	0.2	0.3	0.5
10%凝胶聚合催化剂	0.05	0.1	0.15	0.2	0.3	0.5
TEMED	0.002	0.004	0.006	0.008	0.012	0.02

注：如果配制非变性胶，参考上述配方，不加10%SDS即可配制成非变性PAGE胶。
3.按照如下表格配制SDS-PAGE的浓缩胶(也称堆积胶、积层胶或上层胶)：

成分	配制不同体积SDS-PAGE浓缩胶所需各成分的体积(毫升)
5%胶	2	3	4	6	8	10
蒸馏水	1.4	2.1	2.7	4.1	5.5	6.8
30%Acr-Bis(29:1)	0.33	0.5	0.67	1.0	1.3	1.7
1M Tris, pH6.8	0.25	0.38	0.5	0.75	1.0	1.25
10%SDS	0.02	0.03	0.04	0.06	0.08	0.1
10%凝胶聚合催化剂	0.02	0.03	0.04	0.06	0.08	0.1
TEMED	0.002	0.003	0.004	0.006	0.008	0.01

注：通常10-30分钟内胶会凝固。注：具体的凝固时间和温度及光照有关，说明书中10%凝胶聚合催化剂和TEMED的正常推荐用量是室温为25℃时的推荐用量。为达到与25℃时相近的凝固时间，当室温低于25℃时，可以适当同时加大10%凝胶聚合催化剂和TEMED的用量，例如20℃时建议用量是正常推荐用量的1.5倍，15℃时建议用量是正常推荐用量的2倍。

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Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro.

FRONT NEUROSCI-SWITZ . 2017 Oct 26;11:583. (IF 3.707)

59. Huang L, Deng M, Zhang S, Lu S, Gui X, Fang Y.

β-asarone and levodopa coadministration increases striatal levels of dopamine and levodopa and improves behavioral competence in Parkinson's rat by enhancing dopa decarboxylase activity.

Biomed Pharmacother . 2017 Oct;94:666-678. (IF 4.545)

60. Deng L, Liu T, Zhang B, Wu H, Zhao J, Chen J.

Forkhead box C1 is targeted by microRNA-133b and promotes cell proliferation and migration in osteosarcoma.

Exp Ther Med . 2017 Oct;14(4):2823-2830. (IF 1.785)

61. Zhang J, Wen X, Liu N, Li YQ, Tang XR, Wang YQ, He QM, Yang XJ, Zhang PP, Ma J, Sun Y.

Epigenetic mediated zinc finger protein 671 downregulation promotes cell proliferation and tumorigenicity in nasopharyngeal carcinoma by inhibiting cell cycle arrest.

J EXP CLIN CANC RES . 2017 Oct 19;36(1):147. (IF 7.068)

62. Wu X, Zhou H, Yue B, Li M, Liu F, Qiu C, Chen B, Ma X.

Upregulation of microRNA-25-3p inhibits proliferation, migration and invasion of osteosarcoma cells in vitro by directly targeting SOX4.

Mol Med Rep . 2017 Oct;16(4):4293-4300. (IF 2.1)

63. Zhao Y, Yang L, Huang Z, Lin L, Zheng G.

Synergistic effects of caffeine and catechins on lipid metabolism in chronically fed mice via the AMP-activated protein kinase signaling pathway.

Eur J Nutr . 2017 Oct;56(7):2309-2318. (IF 4.664)

64. Zhao M, Tang S, Xin J, Liu D.

Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia.

Exp Ther Med . 2017 Nov;14(5):4033-4040. (IF 1.785)

65. Fang D, He Y, Luan Z.

Simvastatin augments activation of liver regeneration through attenuating transforming growth factor-β1 induced-apoptosis in obstructive jaundice rats.

Exp Ther Med . 2017 Nov;14(5):4839-4845. (IF 1.785)

66. Du WL, Fang Q, Chen Y, Teng JW, Xiao YS, Xie P, Jin B, Wang JQ.

Effect of silencing the T-Box transcription factor TBX2 in prostate cancer PC3 and LNCaP cells.

Mol Med Rep . 2017 Nov;16(5):6050-6058. (IF 2.1)

67. Tao B, Yi J, Huang C, Xu W, Qin C, Chen L, Chen J, Gao Y, Wang R.

microRNA-96 regulates the proliferation of nucleus pulposus cells by targeting ARID2/AKT signaling.

Mol Med Rep . 2017 Nov;16(5):7553-7560. (IF 2.1)

68. Wang TT, Yuan JH, Ma JZ, Yang WJ, Liu XN, Yin YP, Liu Y, Pan W, Sun SH.

CTGF secreted by mesenchymal-like hepatocellular carcinoma cells plays a role in the polarization of macrophages in hepatocellular carcinoma progression.

Biomed Pharmacother . 2017 Nov;95:111-119. (IF 4.545)

69. Huang B, Tian Y, Zhang W, Ma Y, Yuan Y, Liu C.

Strontium doping promotes bioactivity of rhBMP-2 upon calcium phosphate cement via elevated recognition and expression of BMPR-IA.

COLLOID SURFACE B . 2017 Nov 1;159:684-695. (IF 4.389)

70. He X, Li T, Kang N, Zeng H, Ren S, Zong D, Li J, Cai S, Chen P, Chen Y.

The protective effect of PRMT6 overexpression on cigarette smoke extract-induced murine emphysema model.

INT J CHRONIC OBSTR . 2017 Nov 3;12:3245-3254. (IF 2.772)

71. Liang B, Xiao T, Long J, Liu M, Li Z, Liu S, Yang J.

Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy.

Int J Mol Med . 2017 Dec;40(6):1781-1791. (IF 3.098)

72. Lin Y, Wang X, Yu Y, Liu W, Xie F, Ouyang X, Huang Q.

Expression and prognostic significance of cyclin-dependent kinase inhibitor 1A in patients with resected gastric adenocarcinoma.

Oncol Lett . 2017 Dec;14(6):7473-7482. (IF 2.311)

73. Zhang C, Yu P, Zhu L, Zhao Q, Lu X, Bo S.

Blockade of α7 nicotinic acetylcholine receptors inhibit nicotine-induced tumor growth and vimentin expression in non-small cell lung cancer through MEK/ERK signaling way.

Oncol Rep . 2017 Dec;38(6):3309-3318. (IF 3.417)

74. Chang M, Wu M, Li H.

Curcumin combined with glycyrrhetinic acid inhibits the development of hepatocellular carcinoma cells by down-regulating the PTEN/PI3K/AKT signalling pathway.

Am J Transl Res . 2017 Dec 15;9(12):5567-5575. (IF 3.375)

75. Li E, Zhao Z, Ma B, Zhang J.

Long noncoding RNA HOTAIR promotes the proliferation and metastasis of osteosarcoma cells through the AKT/mTOR signaling pathway.

Exp Ther Med . 2017 Dec;14(6):5321-5328. (IF 1.785)

76. Huang W, Cheng P, Yu K, Han Y, Song M, Li Y.

Hyperforin attenuates aluminum-induced Aβ production and Tau phosphorylation via regulating Akt/GSK-3β signaling pathway in PC12 cells.

Biomed Pharmacother . 2017 Dec;96:1-6. (IF 4.545)

77. Jiao S, Liu Y, Yao Y, Teng J.

miR-124 promotes proliferation and differentiation of neuronal stem cells through inactivating Notch pathway.

Cell Biosci . 2017 Dec 11;7:68. (IF 5.026)

78. Wen L, Jiang X, Sun J, Li X, Li X, Tian L, Li Y, Bai W.

Cyanidin-3-O-glucoside promotes the biosynthesis of progesterone through the protection of mitochondrial function in Pb-exposed rat leydig cells.

Food Chem Toxicol . 2018 Feb;112:427-434. (IF 4.679)

79. Wang LM, Zhao N, Zhang J, Sun QF, Yang CZ, Yang PS.

Tumor necrosis factor-alpha inhibits osteogenic differentiation of pre-osteoblasts by downregulation of EphB4 signaling via activated nuclear factor-kappaB signaling pathway.

J Periodontal Res . 2018 Feb;53(1):66-72. (IF 2.926)

80. Pu T, Liu Y, Xu R, Li F, Chen S, Sun K.

Identification of ZFPM2 mutations in sporadic conotruncal heart defect patients.

Mol Genet Genomics . 2018 Feb;293(1):217-223. (IF 2.797)

81. Wang N, Zhang Q, Luo L, Ning B, Fang Y.

β-asarone inhibited cell growth and promoted autophagy via P53/Bcl-2/Bclin-1 and P53/AMPK/mTOR pathways in Human Glioma U251 cells.

J Cell Physiol . 2018 Mar;233(3):2434-2443. (IF 5.546)

82. Ouyang G, Xiong L, Liu Z, Lam B, Bui B, Ma L, Chen X, Zhou P, Wang K, Zhang Z, Huang H, Miao X, Chen W, Wen Y.

Inhibition of autophagy potentiates the apoptosis-inducing effects of photodynamic therapy on human colon cancer cells.

PHOTODIAGN PHOTODYN . 2018 Mar;21:396-403. (IF 2.894)

83. Xia PP, Li LJ, Qi RD, Shi JJ, Ju WZ, Chen ML.

Electrical and histological remodeling of the pulmonary vein in 2K1C hypertensive rats: Indication of initiation and maintenance of atrial fibrillation.

Anatol J Cardiol . 2018 Mar;19(3):169-175. (IF 1.223)

84. Wang S,Zhao Z,Fan Y,Zhang M,Feng X,Lin J,Hu J,Cheng Z,Sun C,Liu T,Xiong Z,Yang Z,Wang H,Sun D

Mst1 inhibits Sirt3 expression and contributes to diabetic cardiomyopathy through inhibiting Parkin-dependent mitophagy.

BBA-MOL BASIS DIS . 2018 Apr 17. pii: S0925-4439(18)30133-9. (IF 4.352)

85. Cui S, Sun Y, Liu Y, Liu C, Wang J, Hao G, Sun Q.

[Corrigendum] MicroRNA-137 has a suppressive role in liver cancer via targeting EZH2.

Mol Med Rep . 2018 May;17(5):7460. (IF 2.1)

86. Liang H,Sun H,Yang J,Yi C

miR‑145‑5p reduces proliferation and migration of hepatocellular carcinoma by targeting KLF5.

Mol Med Rep . 2018 Jun;17(6):8332-8338. (IF 2.1)

87. Yu G,Chen J,Li S,Pu P,Huang W,Zhao Y,Peng X,Wang R,Lei H

RING finger protein 10 prevents neointimal hyperplasia by promoting apoptosis in vitro and in vivo.

Life Sci . 2018 Sep 1;208:325-332. (IF 3.647)

88. Feng Y,Guo X,Huang X,Wu M,Li X,Wu S,Luo X

Metformin reverses stem cell‑like HepG2 sphere formation and resistance to sorafenib by attenuating epithelial‑mesenchymal transformation.

Mol Med Rep . 2018 Oct;18(4):3866-3872. (IF 2.1)

89. Wang N,Han Y,Luo L,Zhang Q,Ning B,Fang Y

β-asarone induces cell apoptosis, inhibits cell proliferation and decreases migration and invasion of glioma cells.

Biomed Pharmacother . 2018 Oct;106:655-664. (IF 4.545)

90. Qiu X,Guo Q,Liu X,Luo H,Fan D,Deng Y,Cui H,Lu C,Zhang G,He X,Lu A

Pien Tze Huang Alleviates Relapsing-Remitting Experimental Autoimmune Encephalomyelitis Mice by Regulating Th1 and Th17 Cells.

Front Pharmacol . 2018 Oct 31;9:1237. (IF 4.225)

91. Xiao LY,Wang XR,Yang JW,Ye Y,Zhu W,Cao Y,Ma SM,Liu CZ

Acupuncture Prevents the Impairment of Hippocampal LTP Through β1-AR in Vascular Dementia Rats.

Mol Neurobiol. 2018 Oct;55(10):7677-7690. (IF 4.5)

92. Liu H,Li X,Yu WQ,Liu CX

Upregulated EFNB2 and EPHB4 promotes lung development in a nitrofen-induced congenital diaphragmatic hernia rat model.

Int J Mol Med . 2018 Nov;42(5):2373-2382. (IF 3.098)

93. Tian X,Wang F,Luo Y,Ma S,Zhang N,Sun Y,You C,Tang G,Li S,Gong Y,Xie C

Protective Role of Nuclear Factor-Erythroid 2-Related Factor 2 Against Radiation-Induced Lung Injury and Inflammation.

Front Oncol . 2018 Nov 23;8:542. (IF 4.848)

94. Li SS,Zhang MX,Wang Y,Wang W,Zhao CM,Sun XM,Dong GK,Li ZR,Yin WJ,Zhu B,Cai HX

Reduction of PGRN increased fibrosis during skin wound healing in mice.

Histol Histopathol . 2018 Dec 18:18076. (IF 2.021)

95. Gao Z,Zhao GS,Lv Y,Peng D,Tang X,Song H,Guo QN

Anoikis‑resistant human osteosarcoma cells display significant angiogenesis by activating the Src kinase‑mediated MAPK pathway.

Oncol Rep. 2019 Jan;41(1):235-245. (IF 3.417)

96. Zhou ZQ,Chen Y,Chai M,Tao R,Lei YH,Jia YQ,Shu J,Ren J,Li G,Wei WX,Han YD,Han Y

Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose‑derived stem cells into fibroblasts.

Int J Mol Med. 2019 Feb;43(2):890-900. (IF 3.098)

97. Wu XB,Feng X,Chang QM,Zhang CW,Wang ZF,Liu J,Hu ZQ,Liu JZ,Wu WD,Zhang ZP,Liu XQ

Cross-talk among AFAP1-AS1, ACVR1 and microRNA-384 regulates the stemness of pancreatic cancer cells and tumorigenicity in nude mice.

J EXP CLIN CANC RES. 2019 Feb 28;38(1):107. (IF 7.068)

98. Huang X,Han X,Huang Z,Yu M,Zhang Y,Fan Y,Xu B,Zhou K,Song L,Wang X,Lu C,Xia Y

Maternal pentachlorophenol exposure induces developmental toxicity mediated by autophagy on pregnancy mice.

ECOTOX ENVIRON SAFE. 2019 Mar;169:829-836. (IF 4.872)

99. Wang C,Ren YL,Zhai J,Zhou XY,Wu J

Down-regulated LAMA4 inhibits oxidative stress-induced apoptosis of retinal ganglion cells through the MAPK signaling pathway in rats with glaucoma.

Cell Cycle. 2019 May;18(9):932-948. (IF 3.699)

100. Xing Y,Ren S,Ai L,Sun W,Zhao Z,Jiang F,Zhu Y,Piao D

ZNF692 promotes colon adenocarcinoma cell growth and metastasis by activating the PI3K/AKT pathway.

Int J Oncol. 2019 May;54(5):1691-1703. (IF 3.899)

101. Chen YZ,Sun DQ,Zheng Y,Zheng GK,Chen RQ,Lin M,Huang LF,Huang C,Song D,Wu BQ

WISP1 silencing confers protection against epithelial-mesenchymal transition of renal tubular epithelial cells in rats via inactivation of the wnt/β-catenin signaling pathway in uremia.

J Cell Physiol . 2019 Jun;234(6):9673-9686. (IF 5.546)

102. Shen Y,Chen Q,Li L

Endostar regulates EMT, migration and invasion of lung cancer cells through the HGF-Met pathway.

MOL CELL PROBE. 2019 Jun;45:57-64. (IF 1.951)

103. Zuo R,Wang Y,Li J,Wu J,Wang W,Li B,Sun C,Wang Z,Shi C,Zhou Y,Liu M,Zhang C

Rapamycin Induced Autophagy Inhibits Inflammation-Mediated Endplate Degeneration by Enhancing Nrf2/Keap1 Signaling of Cartilage Endplate Stem Cells.

Stem Cells. 2019 Jun;37(6):828-840. (IF 6.022)

104. Liu AH,Chu M,Wang YP

Up-Regulation of Trem2 Inhibits Hippocampal Neuronal Apoptosis and Alleviates Oxidative Stress in Epilepsy via the PI3K/Akt Pathway in Mice.

Neurosci Bull. 2019 Jun;35(3):471-485. (IF 4.326)

105. Liu B,Zhang Q,Wu X,Fu Y,Wang H,Guan Y,Yang S,Liu Y,Cao W,Wang J

Effect of Bletilla striata on the Prevention of Postoperative Peritoneal Adhesions in Abrasion-Induced Rat Model.

EVID-BASED COMPL ALT. 2019 Jun 10;2019:9148754. (IF 1.813)

106. Yang YH,Liu TH,Zhang LD,Chen ZY,Huang XS

Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis.

EVID-BASED COMPL ALT. 2019 Jun 16;2019:7164916. (IF 1.813)

107. Zhuang L,Qu H,Cong J,Dai H,Liu X

MiR-181c affects estrogen-dependent endometrial carcinoma cell growth by targeting PTEN.

Endocr J. 2019 Jun 28;66(6):523-533. (IF 1.952)

108. Wang D,Hu Y

Long Non-coding RNA PVT1 Competitively Binds MicroRNA-424-5p to Regulate CARM1 in Radiosensitivity of Non-Small-Cell Lung Cancer.

MOL THER-NUCL ACIDS. 2019 Jun 7;16:130-140. (IF 7.032)

109. Ji Wei Li,Li Wei,Zhijun Han,Zhong Chen

Mesenchymal Stromal Cells-Derived Exosomes Alleviate Ischemia/Reperfusion Injury in Mouse Lung by Transporting Anti-Apoptotic miR-21-5p

Eur J Pharmacol. 2019 Jun 5;852:68-76.;doi: 10.1016/j.ejphar.2019.01.022. (IF 3.263)

110. Yuan-Zhen Chen,Dan-Qin Sun,Yi Zheng,Guang-Kuai Zheng,Rong-Quan Chen,Mei Lin,Lian-Fang Huang,Cong Huang,Dan Song,Ben-Qing Wu

WISP1 Silencing Confers Protection Against Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells in Rats via Inactivation of the Wnt/β-Catenin Signaling Pathway in Uremia

J Cell Physiol. 2019 Jun;234(6):9673-9686.;doi: 10.1002/jcp.27654. (IF 5.546)

111. Tian X,An R,Luo Y,Li M,Xu L,Dong Z

Tamibarotene Improves Hippocampus Injury Induced by Focal Cerebral Ischemia-Reperfusion via Modulating PI3K/Akt Pathway in Rats.

J STROKE CEREBROVASC . 2019 Jul;28(7):1832-1840. (IF 1.598)

112. Chen JX,Xu X,Zhang S

Silence of long noncoding RNA NEAT1 exerts suppressive effects on immunity during sepsis by promoting microRNA-125-dependent MCEMP1 downregulation.

IUBMB Life. 2019 Jul;71(7):956-968. (IF 3.244)

113. Yang QS,Li B,Xu G,Yang SQ,Wang P,Tang HH,Liu YY

Long noncoding RNA LINC00483/microRNA-144 regulates radiosensitivity and epithelial-mesenchymal transition in lung adenocarcinoma by interacting with HOXA10.

J Cell Physiol. 2019 Jul;234(7):11805-11821. (IF 5.546)

114. Zhou GF,Jiang YH,Ma DF,Wang YC,Yang JL,Chen JY,Chi CY,Han XW,Li ZY,Li X

Xiao-Qing-Long Tang Prevents Cardiomyocyte Hypertrophy, Fibrosis, and the Development of Heart Failure with Preserved Ejection Faction in Rats by Modulating the Composition of the Gut Microbiota.

Biomed Res Int. 2019 Jul 18;2019:9637479. (IF 2.276)

115. Cao J,Zhang C,Jiang GQ,Jin SJ,Gao ZH,Wang Q,Yu DC,Ke AW,Fan YQ,Li DW,Wang AQ,Bai DS

Expression of GLS1 in intrahepatic cholangiocarcinoma and its clinical significance.

Mol Med Rep. 2019 Aug;20(2):1915-1924. (IF 2.1)

116. Su J,Wang J,Luo J,Li H

Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation.

MOL CELL PROBE. 2019 Aug;46:101415. (IF 1.951)

117. Li S,Cao H,Shen D,Chen C,Xing S,Dou F,Jia Q

Efficacy of Shoushen granule on adenosine triphosphate binding cassette transporter A1, proprotein convertase subtilisin/kexin type 9 and toll-like receptor 4/nuclear factor kappa-B signaling pathway

J Tradit Chin Med. 2019 Aug;39(4):524-534. (IF 0.676)

118. Ni T,Gao F,Zhang J,Lin H,Luo H,Chi J,Guo H

Impaired autophagy mediates hyperhomocysteinemia-induced HA-VSMC phenotypic switching.

J Mol Histol. 2019 Aug;50(4):305-314. (IF 2.531)

119. Sun Y,Lei B,Huang Q

SOX18 Affects Cell Viability, Migration, Invasiveness, and Apoptosis in Hepatocellular Carcinoma (HCC) Cells by Participating in Epithelial-to-Mesenchymal Transition (EMT) Progression and Adenosine Mo

MED SCI MONITOR. 2019 Aug 20;25:6244-6254. (IF 1.918)

120. Li Q,Liu B,Breitkopf-Heinlein K,Weng H,Jiang Q,Dong P,Dooley S,Xu K,Ding H

Adenovirus‑mediated overexpression of bone morphogenetic protein‑9 promotes methionine choline deficiency‑induced non‑alcoholic steatohepatitis in non‑obese mice.

Mol Med Rep. 2019 Sep;20(3):2743-2753. (IF 2.1)

121. Jia Q,Cao H,Shen D,Li S,Yan L,Chen C,Xing S,Dou F

Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1.

Int J Mol Med. 2019 Sep;44(3):893-902. (IF 3.098)

122. Ren L,Qi K,Zhang L,Bai Z,Ren C,Xu X,Zhang Z,Li X

Glutathione Might Attenuate Cadmium-Induced Liver Oxidative Stress and Hepatic Stellate Cell Activation.

Biol Trace Elem Res. 2019 Oct;191(2):443-452. (IF 2.639)

123. Gao J,Yin X,Yu X,Dai C,Zhou F

Long noncoding LINC01551 promotes hepatocellular carcinoma cell proliferation, migration, and invasion by acting as a competing endogenous RNA of microRNA-122-5p to regulate ADAM10 expression.

J Cell Biochem. 2019 Oct;120(10):16393-16407. (IF 4.237)

124. Cao H,Jia Q,Shen D,Yan L,Chen C,Xing S

Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE-/- mice.

Exp Ther Med. 2019 Oct;18(4):2451-2458. (IF 1.785)

125. Li YJ,Yang Z,Wang YY,Wang Y

Long noncoding RNA ZNF667-AS1 reduces tumor invasion and metastasis in cervical cancer by counteracting microRNA-93-3p-dependent PEG3 downregulation.

Mol Oncol. 2019 Nov;13(11):2375-2392. (IF 6.574)

126. Yan R,Jiang Y,Lai B,Lin Y,Wen J

The positive feedback loop FOXO3/CASC11/miR-498 promotes the tumorigenesis of non-small cell lung cancer.

BIOCHEM BIOPH RES CO. 2019 Nov 12;519(3):518-524. (IF 2.985)

127. Huang D,Liu M,Jiang Y

Mitochonic acid-5 attenuates TNF-α-mediated neuronal inflammation via activating Parkin-related mitophagy and augmenting the AMPK-Sirt3 pathways.

J Cell Physiol. 2019 Dec;234(12):22172-22182. (IF 5.546)

128. Hua X,Liu Z,Zhou M,Tian Y,Zhao PP,Pan WH,Li CX,Huang XX,Liao ZX,Xian Q,Chen B,Hu Y,Leng L,Fang XW,Yu LN

LSAMP-AS1 binds to microRNA-183-5p to suppress the progression of prostate cancer by up-regulating the tumor suppressor DCN.

EBioMedicine. 2019 Dec;50:178-190. (IF 5.736)

129. Qin S,Wang H,Liu G,Mei H,Chen M

miR‑21‑5p ameliorates hyperoxic acute lung injury and decreases apoptosis of AEC II cells via PTEN/AKT signaling in rats.

Mol Med Rep. 2019 Dec;20(6):4953-4962. (IF 2.1)

130. Jia Q,Cao H,Shen D,Yan L,Chen C,Xing S

Fisetin, via CKIP-1/REGγ, limits oxidized LDL-induced lipid accumulation and senescence in RAW264.7 macrophage-derived foam cells.

Eur J Pharmacol. 2019 Dec 15;865:172748. (IF 3.263)

131. Cao H,Jia Q,Yan L,Chen C,Xing S,Shen D

Quercetin Suppresses the Progression of Atherosclerosis by Regulating MST1-Mediated Autophagy in ox-LDL-Induced RAW264.7 Macrophage Foam Cells.

Int J Mol Sci. 2019 Dec 3;20(23). pii: E6093. (IF 4.556)

132. Jie Luo,Huaiming Wang,Huanjie Chen,Guolian Gan,Yifeng Zheng

CLDN4 Silencing Promotes Proliferation and Reduces Chemotherapy Sensitivity of Gastric Cancer Cells Through Activation of the PI3K/Akt Signalling Pathway

Exp Physiol. 2019 Dec 19.;doi: 10.1113/EP088112. (IF 2.431)

133. Shan-Shan Li,Hui Cao,Ding-Zhu Shen,Chuan Chen,San-Li Xing,Fang-Fang Dou,Qing-Ling Jia

Effect of Quercetin on Atherosclerosis Based on Expressions of ABCA1, LXR-α and PCSK9 in ApoE -/- Mice

Chin J Integr Med. 2020 Feb;26(2):114-121.;doi: 10.1007/s11655-019-2942-9. (IF 1.545)

134. Wenfeng Xie,Lei Chen,Li Chen,Qiuye Kou

Silencing of long non-coding RNA MALAT1 suppresses inflammation in septic mice: role of microRNA-23a in the down-regulation of MCEMP1 expression

Inflamm Res. 2020 Feb;69(2):179-190.;doi: 10.1007/s00011-019-01306-z. (IF 3.174)

135. Wang J,Zuo Y,Zhuang K,Luo K,Yan X,Li J,Zhang JH,Liu F

Recombinant Human Milk Fat Globule-Epidermal Growth Factor 8 Attenuates Microthrombosis after Subarachnoid Hemorrhage in Rats.

J STROKE CEREBROVASC . 2020 Mar;29(3):104536. (IF 1.598)

136. Xicheng Wang,Xining Wang,Yunxiuxiu Xu,Maolin Yan,Wenxin Li,Jie Chen,Tao Chen

Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation

Cancer Cell Int. 2020 Mar 24;20:91.;doi: 10.1186/s12935-020-01172-4. (IF 4.175)

137. Yuanyuan Li,Jiapo Li,Yue Hou,Ling Huang,Yue Bian,Guiyu Song,Chong Qiao

Circadian clock gene Clock is involved in the pathogenesis of preeclampsia through hypoxia

Life Sci. 2020 Apr 15;247:117441.;doi: 10.1016/j.lfs.2020.117441. (IF 3.647)

138. Yanjun Li,Ruimin Guo,Mengying Zhang,Peng Chen,Jingxin Li,Yanni Sun

Protective effect of emodin on intestinal epithelial tight junction barrier integrity in rats with sepsis induced by cecal ligation and puncture

Exp Ther Med. 2020 Jun;19(6):3521-3530.;doi: 10.3892/etm.2020.8625. (IF 1.785)

139. Xidan Zhu,Jia Feng,Wenguang Fu,Xiaojia Shu,Xue Wan,Jinbo Liu

Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following β‑catenin silencing

Int J Mol Med. 2020 Jun;45(6):1838-1850.;doi: 10.3892/ijmm.2020.4543. (IF 3.098)

140. Ping Wang,Jiming Xu,Weijing You,Yongfeng Hou,Shuiliang Wang,Yujie Ma,Jianming Tan,Zengli Zhang,Wentao Hu,Bingyan Li

Knockdown of CYP24A1 Aggravates 1α,25(OH) 2 D 3-Inhibited Migration and Invasion of Mouse Ovarian Epithelial Cells by Suppressing EMT

Front Oncol. 2020 Jul 29;10:1258.;doi: 10.3389/fonc.2020.01258. (IF 4.848)

141. Bing Liu,Lihong Yan,Xuefei Jiao,Xiaozhi Sun,Zonggang Zhao,Junwei Yan,Mingjin Guo,Yunjin Zang

Lycopene Alleviates Hepatic Hypoxia/Reoxygenation Injury Through Nrf2/HO-1 Pathway in AML12 Cell

J Interferon Cytokine Res. 2020 Aug;40(8):406-417.;doi: 10.1089/jir.2020.0038.

142. Kun Ma,Chuan Zhang,Wuyin Li

TGF-β is associated with poor prognosis and promotes osteosarcoma progression via PI3K/Akt pathway activation

Cell Cycle. 2020 Sep;19(18):2327-2339.;doi: 10.1080/15384101.2020.1805552. (IF 3.699)

143. Yuan Bian,Qiu Li,Qiaolian Li,Ruigen Pan

Silencing of BRF2 inhibits the growth and metastasis of lung cancer cells

Mol Med Rep. 2020 Sep;22(3):1767-1774.;doi: 10.3892/mmr.2020.11285. (IF 2.1)

144. Xiaofeng Yang,Yanli Song,Yuexi Sun,Mengmeng Wang,Yang Xiang

Down-regulation of miR-361-5p promotes the viability, migration and tube formation of endothelial progenitor cells via targeting FGF1

Biosci Rep. 2020 Oct 30;40(10):BSR20200557.;doi: 10.1042/BSR20200557.

145. Yufeng Han,Nanbu Wang,Jian Kang,Yongqi Fang

β-Asarone improves learning and memory in Aβ 1-42-induced Alzheimer's disease rats by regulating PINK1-Parkin-mediated mitophagy

Metab Brain Dis. 2020 Oct;35(7):1109-1117.;doi: 10.1007/s11011-020-00587-2. (IF 2.726)

146. Niannian Yang,Hao Cheng,Qiao Mo,Xiaobiao Zhou,Minqiang Xie

miR‑155‑5p downregulation inhibits epithelial‑to‑mesenchymal transition by targeting SIRT1 in human nasal epithelial cells

Mol Med Rep. 2020 Nov;22(5):3695-3704.;doi: 10.3892/mmr.2020.11468. (IF 2.1)

147. Guangyu Qian,Xiaoyan Jin,Louwei Zhang

LncRNA FENDRR Upregulation Promotes Hepatic Carcinoma Cells Apoptosis by Targeting miR-362-5p Via NPR3 and p38-MAPK Pathway

Cancer Biother Radiopharm. 2020 Nov;35(9):629-639.;doi: 10.1089/cbr.2019.3468.

148. Yong Yang,Yong Zhou,Yiyong Wang,Xianglong Wei,Lihao Wu,Tingzhong Wang,Aiqun Ma

Exendin-4 reverses high glucose-induced endothelial progenitor cell dysfunction via SDF-1β/CXCR7-AMPK/p38-MAPK/IL-6 axis

Acta Diabetol. 2020 Nov;57(11):1315-1326.;doi: 10.1007/s00592-020-01551-3. (IF 3.418)

149. Jing Huo,Taibin Liu,Fenghua Li,Xinfeng Song,Xiuli Hou

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo

Mol Med Rep. 2021 Jan;23(1):51.;doi: 10.3892/mmr.2020.11689. (IF 2.1)

150. Cheng Long,Shiqiang Cen,Zhou Zhong,Chang Zhou,Gang Zhong

FOXO3 is targeted by miR-223-3p and promotes osteogenic differentiation of bone marrow mesenchymal stem cells by enhancing autophagy

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