| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IF, IHC, ICC | H, M, Rb | 26kDa | 26kDa |
WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry. H, Human; M, Mouse; Rb, Rabbit.
BeyoAb™系列抗体为经深入测试的优质抗体，并配套提供Western一抗稀释液，可用于Western或其它适当用途的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整，NT, not tested)。
| WB | IP | IF | IHC | ICC | FC | ChIP |
| 1:1000-1:2000 | NT | 1:50-1:200 | 1:50 | 1:50-1:200 | NT | NT |
本抗体相关应用的代表性检测效果图片请参见Fig. 1。
Fig. 1. BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody (AB2017) was used for Western blot, immunofluorescence and immunohistochemistry.
A. Western analysis of lysates from indicated cell lines using BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody (AB2017) at 1:1000 dilution and BeyoWB™ HRP-labeled Goat Anti-Rabbit IgG (H+L) (P0948) at 1:500 dilution.20µg protein per lane was loaded and analyzed by BeyoWB™ 80min Electrophoresis, Transfer and Western Blot Kit (P0002). Exposure time, 2s.
B. Immunofluorescence analysis of HeLa cells using BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody (AB2017) at a dilution of 1:50 and Cy3-labeled Goat Anti-Rabbit IgG (H+L) (A0516) at 1:250 dilution. a. Brightfield. b. Immunofluorescence image for Bcl-2. c. Nuclear staining using Antifade Mounting Medium with DAPI (P0131). d, Merged image for b and c.
C. Immunohistochemistry of a section from paraffin-embedded mouse spleen tissue using BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody (AB2017) at a dilution of 1:50 (bottom panel) compared to a negative control without primary antibody (Top panel). HRP-labeled Goat Anti-Rabbit IgG (H+L) (A0208) was used as secondary antibody at 1:1000 dilution.
抗体详细信息如下:
| About this Antibody | |
| Name | BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody |
| Category | Polyclonal antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | HeLa; 293T |
| Cellular location | - |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Recombinant protein |
| Sequence | - |
| Gene ID | 596 |
| SwissProt | P10415 |
| Synonyms | BCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2 |
| Category | Regulation of Apoptosis; Inhibition of Apoptosis; Mitochondrial Control of Apoptosis;Death Receptor Signaling |
| Background | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release [1]. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events [2]. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions [3]. |
| 产品编号 | 产品名称 | 包装 |
| AB2017-50μl | BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AB2017-200μl | BeyoAb™ Bcl-2 Rabbit Polyclonal Antibody | 200μl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
-20ºC保存，至少一年有效。Western一抗稀释液也可以4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4ºC保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。