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多肽与蛋白> 蛋白检测> 免疫染色
免疫染色封闭液
产品编号: P0102
产品包装:100ml
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价格: ¥ 156.00
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产品编号 产品名称 产品包装 产品价格
P0102 免疫染色封闭液 100ml 156.00元

碧云天生产的免疫染色封闭液(Immunol Staining Blocking Buffer)可以用于免疫染色时切片或细胞样品的封闭。封闭后,可以减小后续的一抗或二抗和样品的非特异性结合,降低背景,增强信噪比。
本免疫染色封闭液中含有适量Triton X-100,可以通透细胞膜。经本免疫染色封闭液处理的细胞样品可以检测细胞内的目的蛋白,包括细胞浆内和细胞核内的目的蛋白。
本产品的主要有效成分为经过反复优化的适量BSA和适当的去垢剂等,一方面能有效封闭样品上的非特异性蛋白结合位点,另一方面能有效减少后续一抗和二抗的非特异性结合。
本产品可以用于一抗的稀释,但更推荐免疫染色时一抗稀释专用的免疫染色一抗稀释液(P0103)QuickBlock™免疫染色一抗稀释液(P0262)
关于不同封闭液的比较和选择,请参考碧云天的相关网页:http://www.beyotime.com/support/blocking-buffer.htm
按照每组样品封闭需要5-10毫升封闭液计算,一个包装的免疫染色封闭液可以封闭10-20组样品。
包装清单:

产品编号 产品名称 包装
P0102 免疫染色封闭液 100ml
说明书 1份

保存条件:
4℃保存,一年有效。长期不使用可以-20℃保存。
注意事项:
通常在室温封闭60分钟即可。如果对于一些背景非常高的抗体,可以4℃封闭过夜。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 对于切片或细胞样品,在固定后用免疫染色洗涤液洗涤一次(注:石蜡切片需先脱蜡)。然后就可以用免疫染色封闭液进行封闭。通常建议封闭60分钟。封闭时可以在摇床上缓慢摇动。如果样品在摇动时容易脱落,也可以直接放置在桌面上不进行任何摇动,效果会略差一些,但也可以满足常规的要求。完成封闭后就可以进行一抗孵育等后续操作。
2. 详细的免疫染色操作可以参考我们的相关网页:http://www.beyotime.com/support/immunol-staining.htm

相关产品:
产品编号 产品名称 包装
P0102 免疫染色封闭液 100ml
P0103 免疫染色一抗稀释液 100ml
P0108 免疫荧光染色二抗稀释液 100ml
P0110 免疫染色(非荧光)二抗稀释液 100ml
P0260 QuickBlock™免疫染色封闭液 100ml
P0262 QuickBlock™免疫染色一抗稀释液 100ml
P0265 QuickBlock™免疫荧光染色二抗稀释液 100ml
P0267 QuickBlock™免疫组化染色二抗稀释液 100ml

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