产品编号: P0102
产品包装: 100ml
产品价格: 140.00元
产品编号 产品名称 产品包装 产品价格
P0102 免疫染色封闭液 100ml 140.00元

      碧云天生产的免疫染色封闭液(Immunol Staining Blocking Buffer)可以用于免疫染色时切片或细胞样品的封闭。封闭后,可以减小后续的一抗或二抗和样品的非特异性结合,降低背景,增强信噪比。
      本免疫染色封闭液中含有适量Triton X-100,可以通透细胞膜。经本免疫染色封闭液处理的细胞样品可以检测细胞内的目的

产品编号 产品名称 包装
P0102 免疫染色封闭液 100ml
说明书 1份


1. 对于切片或细胞样品,在固定后用免疫染色洗涤液洗涤一次(注:石蜡切片需先脱蜡)。然后就可以用免疫染色封闭液进行封闭。通常建议封闭60分钟。封闭时可以在摇床上缓慢摇动。如果样品在摇动时容易脱落,也可以直接放置在桌面上不进行任何摇动,效果会略差一些,但也可以满足常规的要求。完成封闭后就可以进行一抗孵育等后续操作。
2. 详细的免疫染色操作可以参考我们的相关网页:http://www.beyotime.com/support/immunol-staining.htm

产品编号 产品名称 包装
P0102 免疫染色封闭液 100ml
P0103 免疫染色一抗稀释液 100ml
P0108 免疫荧光染色二抗稀释液 100ml
P0110 免疫染色(非荧光)二抗稀释液 100ml
P0260 QuickBlock™免疫染色封闭液 100ml
P0262 QuickBlock™免疫染色一抗稀释液 100ml
P0265 QuickBlock™免疫荧光染色二抗稀释液 100ml
P0267 QuickBlock™免疫组化染色二抗稀释液 100ml


1. Zou XH, Li HM, Wang S, Leski M, Yao YC, Yang XD, Huang QJ, Chen GQ.
The effect of 3-hydroxybutyrate methyl ester on learning and memory in mice.
Biomaterials. 2009 Mar;30(8):1532-41.
2. Zhang Z, Wang Q, Li P, Zhou Y, Li S, Yi W, Chen A, Kong P, Hu C.
Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal cancer and its clinical significance.
Cancer Epidemiol. 2009 Aug;33(2):130-6. 
3. Tang S, Wang Y, Zhang D, Gao Y, Ma Y, Yin B, Sun J, Liu J, Zhang Y.
Reprogramming donor cells with oocyte extracts improves in vitro development of nuclear transfer embryos.
Anim Reprod Sci. 2009 Oct;115(1-4):1-9.
4. Zhou F, Hu J, Shao JH, Zou SB, Shen SL, Luo ZQ
Metronomic chemotherapy in combination with antiangiogenic treatment induces mosaic vascularreduction and tumor growth inhibition in hepatocellular carcinoma xenografts.
J Cancer Res Clin Oncol. 2012 Nov;138(11):1879-90.
5. Su J, Wang Y, Li R, Peng H, Hua S, Li Q, Quan F, Guo Z, Zhang Y.
Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo development of SCNTembryos in cattle.
PLoS One. 2012;7(4):e36181.  
6. Yang Z, Liu J, Liu H, Qiu M, Liu Q, Zheng L, Pang M, Quan F, Zhang Y.
Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat Skin Fibroblasts.
Cell Reprogram. 2013 Jun;15(3):195-205.  
7. Liu SL, Zhong SS, Ye DX, Chen WT, Zhang ZY, Deng J.
Repression of G protein-coupled receptor family C group 5 member A is associated with pathologic differentiation grade of oral squamous cell carcinoma.
J Oral Pathol Med. 2013 Nov;42(10):761-8.  
8. Liu J, Wang Y, Su J, Wang L, Li R, Li Q, Wu Y, Hua S, Quan F, Guo Z, Zhang Y.
Effect of the time interval between fusion and activation on epigenetic reprogramming and development of bovinesomatic cell nuclear transfer embryos.
Cell Reprogram. 2013 Apr;15(2):134-42.  
9. Qiu M, Quan F, Han C, Wu B, Liu J, Yang Z, Su F, Zhang Y.
Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal cells and theca cells derivedfrom the goat ovary.
J Steroid Biochem Mol Biol. 2013 Nov;138:325-33.
10. Cong ZX, Wang HD, Wang JW, Zhou Y, Pan H, Zhang DD, Zhu L.
ERK and PI3K signaling cascades induce Nrf2 activation and regulate cell viability partly through Nrf2 in humanglioblastoma cells.
Oncol Rep. 2013 Aug;30(2):715-22. 
11.Wang J, Lu K, Liang F, Li X, Wang L, Yang C, Yan Z, Zhang S, Liu H.
Decreased autophagy contributes to myocardial dysfunction in rats subjected to nonlethal mechanical trauma.
PLoS One. 2013 Aug 19;8(8):e71400.  
12.Peng H, Zhang W, Xiao T, Zhang Y.
Nlrp4g is an oocyte-specific gene but is not required for oocyte maturation in the mouse.
Reprod Fertil Dev.  2014 Jun;26(5):758-68. 
13.Zhang L, Wang H, Zhu J, Xu J, Ding K.
Mollugin induces tumor cell apoptosis and autophagy via the PI3K/AKT/mTOR/p70S6K and ERK signaling pathways.
Biochem Biophys Res Commun. 2014 Jul 18;450(1):247-54.  
14.Cong ZX, Wang HD, Zhou Y, Wang JW, Pan H, Zhang DD, Zhang L, Zhu L.
Temozolomide and irradiation combined treatment-induced Nrf2 activation increases chemoradiation sensitivity in human glioblastoma cells.
J Neurooncol. 2014 Jan;116(1):41-8.
15.Qiu M, Liu J, Han C, Wu B, Yang Z, Su F, Quan F, Zhang Y.
The influence of ovarian stromal/theca cells during in vitro culture on steroidogenesis,proliferation and apoptosis of granulosa cells derived from the goat ovary.
Reprod Domest Anim. 2014 Feb;49(1):170-6. 
16.Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J,Zhang X, Dai S, Sun X.
Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
J Hematol Oncol. 2014 Aug 20;7:62.  
17.Zhu Y, Zhang JJ, Xie KL, Tang J, Liang WB, Zhu R, Zhu Y, Wang B, Tao JQ, Zhi XF, Li Z,Gao WT, Jiang KR, Miao Y, Xu ZK.
Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling.
J Transl Med. 2014 Nov 4;12(1):309.
18.Zhang L, Wang H, Xu J, Zhu J, Ding K.
Inhibition of cathepsin S induces autophagy and apoptosis in human glioblastoma cell lines through ROS-mediated PI3K/AKT/mTOR/p70S6K and JNK signaling pathways.
Toxicol Lett. 2014 Aug 4;228(3):248-59.  
19.Hui K, Yang Y, Shi K, Luo H, Duan J, An J, Wu P, Ci Y, Shi L, Xu C.
The p38 MAPK-regulated PKD1/CREB/Bcl-2 pathway contributes to selenite-induced colorectal cancer cell apoptosis in vitro and in vivo.
Cancer Lett. 2014 Nov 1;354(1):189-99. 
20.Song ZQ, Yang LF, Wang YS, Zhu T, Zhou XM, Yin XM, Yao HQ, Zhao DM.
Overexpression of BAT3 alleviates prion protein fragment PrP106-126-induced neuronal apoptosis.
CNS Neurosci Ther. 2014 Aug;20(8):737-47.  
21.Gao W, Gu Y, Li Z, Cai H, Peng Q, Tu M, Kondo Y, Shinjo K, Zhu Y, Zhang J, Sekido Y, Han B, Qian Z, Miao Y.
miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in pancreatic ductal adenocarcinoma.
Oncogene.  2015 Mar 26;34(13):1629-40.
22.Shen JQ, Yang QL, Xue Y, Cheng XB, Jiang ZH, Yang YC, Chen YD, Zhou XN.
Inducible nitric oxide synthase response and associated cytokine gene expression in the spleen of mice infected with Clonorchis sinensis.
Parasitol Res. 2015 May;114(5):1661-70.
23.Gao W, Gu Y, Li Z, Cai H, Peng Q, Tu M, Kondo Y, Shinjo K, Zhu Y, Zhang J, Sekido Y, Han B, Qian Z, Miao Y. 
miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in pancreatic ductal adenocarcinoma.
Oncogene. 2015 Mar 26;34(13):1629-40.
24.Wang L, Ren C, You J, Fan Y, Wan Y, Zhang Y, Wang F, Huang M.
A novel fluorescence reporter system for the characterization of dairy goat mammary epithelial cells.
Biochem Biophys Res Commun. 2015 Mar 20;458(4):783-9.
25.Zhang L, Wang H, Ding K, Xu J. 
FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells.
Toxicol Lett. 2015 Jul 2;236(1):43-59.
26.Zhang D, Yan H, Hu Y, Zhuang Z, Yu Z, Hang C.
Increased Expression of NLRP3 Inflammasome in Wall of Ruptured and Unruptured Human Cerebral Aneurysms: Preliminary Results.
J Stroke Cerebrovasc Dis. 2015 May;24(5):972-9.
27.Liang W, Zhuo Y, Xiong C, Zheng Y, Chai Y, Yuan R.
Ultrasensitive Cytosensor Based on Self-Enhanced Electrochemiluminescent Ruthenium-Silica Composite Nanoparticles for Efficient Drug Screening with Cell Apoptosis Monitoring.
Anal Chem. 2015 Dec 15;87(24):12363-71.
28.Gao X, Hao S, Yan H, Ding W, Li K, Li J.
Neutrophil extracellular traps contribute to the intestine damage in endotoxemic rats.
J Surg Res. 2015 May 1;195(1):211-8.
29.Hu H, Zhang Y, Zheng N, Cheng J, Wang J. 
The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells.
Anim Sci J. 2016 Jan;87(1):84-91. 
30.Zhang D, Wu W, Yan H, Jiang T, Liu M, Yu Z, Li H, Hang C. 
Upregulation of HMGB1 in wall of ruptured and unruptured human cerebral aneurysms: preliminary results.
Neurol Sci. 2016 Feb;37(2):219-26.
31.Su Z, Zhi X, Zhang Q, Yang L, Xu H, Xu Z.
LncRNA H19 functions as a competing endogenous RNA to regulate AQP3 expression by sponging miR-874in the intestinal barrier.
FEBS Lett. 2016 May;590(9):1354-64.
32.Dang Y, Mu Y, Wang K, Xu K, Yang J, Zhu Y, Luo B.
Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signalingpathway.
Drug Des Devel Ther. 2016 Feb 26;10:851-9.
33.Wang Y, Bi X, Chu Q, Xu T.
Discovery of toll-like receptor 13 exists in the teleost fish: Miiuy croaker (Perciformes, Sciaenidae).
Dev Comp Immunol. 2016 Aug;61:25-33.
34.Song Z, Zhu T, Zhou X, Barrow P, Yang W, Cui Y, Yang L, Zhao D.
REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration andneuronal death partially via LRP6-mediated Wnt-β-catenin signaling.
Oncotarget. 2016 Mar 15;7(11):12035-52.
35.Han J, Wang Y, Chu Q, Xu T.
The evolution and functional characterization of miiuy croaker cytosolic gene LGP2 involved in immuneresponse.
Fish Shellfish Immunol. 2016 Nov;58:193-202.
36.Da Z, Li L, Zhu J, Gu Z, You B, Shan Y, Shi S.
CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE.
J Immunol Res. 2016;2016:2063985.
37.Su WF, Gu Y, Wei ZY, Shen YT, Jin ZH, Yuan Y, Gu XS, Chen G.
Rab27a/Slp2-a complex is involved in Schwann cell myelination.
Neural Regen Res. 2016 Nov;11(11):1830-1838.
38.Xie L, Xiang GH, Tang T, Tang Y, Zhao LY, Liu D, Zhang YR, Tang JT, Zhou S, Wu DH.
Capsaicin and dihydrocapsaicin induce apoptosis in human glioma cells via ROS and Ca2+ mediatedmitochondrial pathway.
Mol Med Rep. 2016 Nov;14(5):4198-4208.
39.Cui Y, Zhao D, Sreevatsan S, Liu C, Yang W, Song Z, Yang L, Barrow P, Zhou X.
Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in aMurine Macrophage Cell Line.
Front Cell Infect Microbiol. 2016 Dec 12;6:182.
40.Hu H, Zhang Y, Zheng N, Cheng J, Wang J.
The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovinemammary epithelial cells.
Anim Sci J. 2016 Jan;87(1):84-91.
41.Xu T, Wang Y, Li J, Shu C, Han J, Chu Q.
Comparative genomic evidence for duplication of TLR1 subfamily and miiuy croaker TLR1 perceives LPSstimulation via MyD88 and TIRAP.
Fish Shellfish Immunol. 2016 Sep;56:336-48.
42.Zhang J, Song X, Cao W, Lu J, Wang X, Wang G, Wang Z, Chen X.
Autophagy and mitochondrial dysfunction in adjuvant-arthritis rats treatment with resveratrol.
Sci Rep. 2016 Sep 9;6:32928.
43.Liu TH, Dong XL, Pan CX, Du GY, Wu YF, Yang JG, Chen P, Lu C, Pan MH.
A newly discovered member of the Atlastin family, BmAtlastin-n, has an antiviral effect against BmNPV inBombyx mori.
Sci Rep. 2016 Jun 29;6:28946.
44.Shu C, Chu Q, Bi D, Wang Y, Xu T.
Identification and functional characterization of miiuy croaker IRF3 as an inducible protein involvedregulation of IFN response.
Fish Shellfish Immunol. 2016 Jul;54:499-506.
45.Fu P, Sun W, Zhang Z.
Molecular cloning, expression and characterization of acylpeptide hydrolase in the silkworm, Bombyx mori.
Gene. 2016 Apr 10;580(1):8-16.
46.Li PZ, Yan GY, Han L, Pang J, Zhong BS, Zhang GM, Wang F, Zhang YL.
Overexpression of STRA8, BOULE, and DAZL Genes Promotes Goat Bone Marrow-Derived MesenchymalStem Cells In Vitro Transdifferentiation Toward Putative Male Germ Cells.
Reprod Sci. 2017 Feb;24(2):300-312.
47.Chen Y, Zhang Q, Ding C, Zhang X, Qiu X, Zhang Z.
Stathmin1 overexpression in hypopharyngeal squamous cell carcinoma: A new promoter in FaDu cellproliferation and migration.
Int J Oncol. 2017 Jan;50(1):31-40.