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试剂
质粒抽提 |DNA纯化胶回收
基因组DNA抽提 |DNA电泳
DNA分子量标准 |菌种与培养
感受态制备 |T载体、质粒 |基因突变
DNA连接 |DNA标记和检测 |内切酶
修饰酶 |反转录 |PCR相关 |RNA相关
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抗体制备 |蛋白电泳
蛋白分子量标准 |一抗 |二抗
Western |免疫沉淀 |免疫染色 |ELISA
细胞凋亡坏死 |细胞增殖 |自噬
细胞培养 |细胞组织染色 |细胞转染
细胞组分分离
EMSA相关 |报告基因相关
一氧化氮相关 |活性氧相关
炎症相关 |代谢相关
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其他试剂盒
常用试剂
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超声破碎仪 |匀浆机
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培养箱 |水浴锅 |干燥箱、烘箱
干式恒温器 |其它温控设备 |制冰机
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组织脱水机 |染色机
其它动物实验仪器
耗材
离心管 |PCR耗材 |冻存管 |枪头
移液管 |巴氏吸管
离心管盒、冻存盒 |冰盒 |离心管架
枪头盒 |培养皿 |培养板 |磁性分离
封板膜 |手套等防护用品 |载玻片
盖玻片 |存储盒 |染色缸染色架
其他染色耗材 |印迹膜与洗膜盒
胶片与暗盒 |滤纸、滤膜、滤器
空柱管 |蓝盖瓶 |其他实验耗材
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 免疫染色封闭液

  产品编号P0102
  产品包装: 100ml
  产品价格: 140.00元
产品编号 产品名称 产品包装 产品价格
P0102 免疫染色封闭液 100ml 140.00元

      碧云天生产的免疫染色封闭液(Immunol Staining Blocking Buffer)可以用于免疫染色时切片或细胞样品的封闭。封闭后,可以减小后续的一抗或二抗和样品的非特异性结合,降低背景,增强信噪比。
      本免疫染色封闭液中含有适量Triton X-100,可以通透细胞膜。经本免疫染色封闭液处理的细胞样品可以检测细胞内的目的
蛋白,包括细胞浆内和细胞核内的目的蛋白。
      本产品的主要有效成分为经过反复优化的适量BSA和适当的去垢剂等,一方面能有效封闭样品上的非特异性蛋白结合位点,另一方面能有效减少后续一抗和二抗的非特异性结合。
      本产品可以用于一抗的稀释,但更推荐免疫染色时一抗稀释专用的免疫染色一抗稀释液(P0103)QuickBlock™免疫染色一抗稀释液(P0262)
      关于不同封闭液的比较和选择,请参考碧云天的相关网页:http://www.beyotime.com/support/blocking-buffer.htm
      按照每组样品封闭需要5-10毫升封闭液计算,一个包装的免疫染色封闭液可以封闭10-20组样品。
包装清单:

产品编号 产品名称 包装
P0102 免疫染色封闭液 100ml
说明书 1份

保存条件:
      4℃保存,一年有效。
注意事项:
      通常在室温封闭60分钟即可。如果对于一些背景非常高的抗体,可以4℃封闭过夜。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 对于切片或细胞样品,在固定后用免疫染色洗涤液洗涤一次(注:石蜡切片需先脱蜡)。然后就可以用免疫染色封闭液进行封闭。通常建议封闭60分钟。封闭时可以在摇床上缓慢摇动。如果样品在摇动时容易脱落,也可以直接放置在桌面上不进行任何摇动,效果会略差一些,但也可以满足常规的要求。完成封闭后就可以进行一抗孵育等后续操作。
2. 详细的免疫染色操作可以参考我们的相关网页:http://www.beyotime.com/support/immunol-staining.htm

相关产品:
产品编号 产品名称 包装
P0102 免疫染色封闭液 100ml
P0103 免疫染色一抗稀释液 100ml
P0108 免疫荧光染色二抗稀释液 100ml
P0110 免疫染色(非荧光)二抗稀释液 100ml
P0260 QuickBlock™免疫染色封闭液 100ml
P0262 QuickBlock™免疫染色一抗稀释液 100ml
P0265 QuickBlock™免疫荧光染色二抗稀释液 100ml
P0267 QuickBlock™免疫组化染色二抗稀释液 100ml

相关产品请点击如下按钮:

使用本产品的文献:
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Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal cancer and its clinical significance.
Cancer Epidemiol. 2009 Aug;33(2):130-6. 
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Reprogramming donor cells with oocyte extracts improves in vitro development of nuclear transfer embryos.
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4. Zhou F, Hu J, Shao JH, Zou SB, Shen SL, Luo ZQ
Metronomic chemotherapy in combination with antiangiogenic treatment induces mosaic vascularreduction and tumor growth inhibition in hepatocellular carcinoma xenografts.
J Cancer Res Clin Oncol. 2012 Nov;138(11):1879-90.
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Oocytes selected using BCB staining enhance nuclear reprogramming and the in vivo development of SCNTembryos in cattle.
PLoS One. 2012;7(4):e36181.  
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Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat Skin Fibroblasts.
Cell Reprogram. 2013 Jun;15(3):195-205.  
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Repression of G protein-coupled receptor family C group 5 member A is associated with pathologic differentiation grade of oral squamous cell carcinoma.
J Oral Pathol Med. 2013 Nov;42(10):761-8.  
8. Liu J, Wang Y, Su J, Wang L, Li R, Li Q, Wu Y, Hua S, Quan F, Guo Z, Zhang Y.
Effect of the time interval between fusion and activation on epigenetic reprogramming and development of bovinesomatic cell nuclear transfer embryos.
Cell Reprogram. 2013 Apr;15(2):134-42.  
9. Qiu M, Quan F, Han C, Wu B, Liu J, Yang Z, Su F, Zhang Y.
Effects of granulosa cells on steroidogenesis, proliferation and apoptosis of stromal cells and theca cells derivedfrom the goat ovary.
J Steroid Biochem Mol Biol. 2013 Nov;138:325-33.
10. Cong ZX, Wang HD, Wang JW, Zhou Y, Pan H, Zhang DD, Zhu L.
ERK and PI3K signaling cascades induce Nrf2 activation and regulate cell viability partly through Nrf2 in humanglioblastoma cells.
Oncol Rep. 2013 Aug;30(2):715-22. 
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Decreased autophagy contributes to myocardial dysfunction in rats subjected to nonlethal mechanical trauma.
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Nlrp4g is an oocyte-specific gene but is not required for oocyte maturation in the mouse.
Reprod Fertil Dev.  2014 Jun;26(5):758-68. 
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14.Cong ZX, Wang HD, Zhou Y, Wang JW, Pan H, Zhang DD, Zhang L, Zhu L.
Temozolomide and irradiation combined treatment-induced Nrf2 activation increases chemoradiation sensitivity in human glioblastoma cells.
J Neurooncol. 2014 Jan;116(1):41-8.
15.Qiu M, Liu J, Han C, Wu B, Yang Z, Su F, Quan F, Zhang Y.
The influence of ovarian stromal/theca cells during in vitro culture on steroidogenesis,proliferation and apoptosis of granulosa cells derived from the goat ovary.
Reprod Domest Anim. 2014 Feb;49(1):170-6. 
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Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
J Hematol Oncol. 2014 Aug 20;7:62.  
17.Zhu Y, Zhang JJ, Xie KL, Tang J, Liang WB, Zhu R, Zhu Y, Wang B, Tao JQ, Zhi XF, Li Z,Gao WT, Jiang KR, Miao Y, Xu ZK.
Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling.
J Transl Med. 2014 Nov 4;12(1):309.
18.Zhang L, Wang H, Xu J, Zhu J, Ding K.
Inhibition of cathepsin S induces autophagy and apoptosis in human glioblastoma cell lines through ROS-mediated PI3K/AKT/mTOR/p70S6K and JNK signaling pathways.
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The p38 MAPK-regulated PKD1/CREB/Bcl-2 pathway contributes to selenite-induced colorectal cancer cell apoptosis in vitro and in vivo.
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Overexpression of BAT3 alleviates prion protein fragment PrP106-126-induced neuronal apoptosis.
CNS Neurosci Ther. 2014 Aug;20(8):737-47.  
21.Gao W, Gu Y, Li Z, Cai H, Peng Q, Tu M, Kondo Y, Shinjo K, Zhu Y, Zhang J, Sekido Y, Han B, Qian Z, Miao Y.
miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in pancreatic ductal adenocarcinoma.
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miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in pancreatic ductal adenocarcinoma.
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24.Wang L, Ren C, You J, Fan Y, Wan Y, Zhang Y, Wang F, Huang M.
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Ultrasensitive Cytosensor Based on Self-Enhanced Electrochemiluminescent Ruthenium-Silica Composite Nanoparticles for Efficient Drug Screening with Cell Apoptosis Monitoring.
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Neutrophil extracellular traps contribute to the intestine damage in endotoxemic rats.
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Anim Sci J. 2016 Jan;87(1):84-91. 
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31.Su Z, Zhi X, Zhang Q, Yang L, Xu H, Xu Z.
LncRNA H19 functions as a competing endogenous RNA to regulate AQP3 expression by sponging miR-874in the intestinal barrier.
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32.Dang Y, Mu Y, Wang K, Xu K, Yang J, Zhu Y, Luo B.
Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signalingpathway.
Drug Des Devel Ther. 2016 Feb 26;10:851-9.
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Discovery of toll-like receptor 13 exists in the teleost fish: Miiuy croaker (Perciformes, Sciaenidae).
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REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration andneuronal death partially via LRP6-mediated Wnt-β-catenin signaling.
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The evolution and functional characterization of miiuy croaker cytosolic gene LGP2 involved in immuneresponse.
Fish Shellfish Immunol. 2016 Nov;58:193-202.
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CXCL13 Promotes Proliferation of Mesangial Cells by Combination with CXCR5 in SLE.
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Rab27a/Slp2-a complex is involved in Schwann cell myelination.
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38.Xie L, Xiang GH, Tang T, Tang Y, Zhao LY, Liu D, Zhang YR, Tang JT, Zhou S, Wu DH.
Capsaicin and dihydrocapsaicin induce apoptosis in human glioma cells via ROS and Ca2+ mediatedmitochondrial pathway.
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Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in aMurine Macrophage Cell Line.
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The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovinemammary epithelial cells.
Anim Sci J. 2016 Jan;87(1):84-91.
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Fish Shellfish Immunol. 2016 Sep;56:336-48.
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Autophagy and mitochondrial dysfunction in adjuvant-arthritis rats treatment with resveratrol.
Sci Rep. 2016 Sep 9;6:32928.
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A newly discovered member of the Atlastin family, BmAtlastin-n, has an antiviral effect against BmNPV inBombyx mori.
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Identification and functional characterization of miiuy croaker IRF3 as an inducible protein involvedregulation of IFN response.
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Molecular cloning, expression and characterization of acylpeptide hydrolase in the silkworm, Bombyx mori.
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