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细胞培养

胰酶细胞消化液
产品编号: C0201
产品包装: 100ml
产品价格: 29.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
C0201 胰酶细胞消化液 100ml 29.00元

      碧云天生产的胰酶细胞消化液(Trypsin-EDTA Solution)含0.25%胰酶和0.02%EDTA。该消化液经过过滤除菌,可以直接用于培养细胞的消化,或者一些组织的消化。
      本胰酶细胞消化液具有方便快速的特点,通常室温消化1分钟左右就可以消化下大多数贴壁细胞。
包装清单:

产品编号 产品名称 包装
C0201 胰酶细胞消化液 100ml
说明书 1份

保存条件:
      4℃保存,一年有效。
注意事项:
      在使用胰酶细胞消化液的过程中要特别注意避免消化液被细菌污染。
      胰酶细胞消化液消化细胞时间不宜过长,否则细胞铺板后生长状况会较差。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。 


使用说明:
1. 贴壁细胞的消化:
a.吸去培养液,用无菌的PBS、Hanks液或无血清培养液洗涤细胞一次,以去除残余的血清。
b.加入少量胰酶细胞消化液,略盖过细胞即可,室温放置30秒至2分钟。不同的细胞消化时间有所不同。
c.显微镜下观察,细胞明显收缩,并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化;或者用枪吹打细胞发现细胞刚好可以被吹打下来。此时吸除胰酶细胞消化液。加入含血清的完全细胞培养液,吹打下细胞,即可直接用于后续实验。
d.如果发现消化不足,则加入胰酶细胞消化液重新消化。
如果发现细胞消化时间过长,未及吹打细胞,细胞已经有部分直接从培养器皿底部脱落,直接用胰酶细胞培养液把细胞全部吹打下来。1000-2000g离心1分钟,沉淀细胞,尽量去除胰酶细胞消化液后,加入含血清的完全培养液重新悬浮细胞,即可用于后续实验。
2. 组织的消化:
a.不同的组织需要消化的时间相差很大,通常以消化后可以充分打散组织为宜。

附录:不同胰酶细胞消化液的比较和选择
1.如果希望消化能力比较强,推荐选择C0201胰酶细胞消化液C0203 胰酶细胞消化液(含酚红),这两种胰酶细胞消化液都含有EDTA,消化能力相对更强一些。
2.如果希望观察比较方便,推荐选择含酚红的C0203 胰酶细胞消化液(含酚红)C0207 胰酶细胞消化液(含酚红,不含EDTA)
3.对于酚红可能会干扰后续的测试分析,推荐选择不含酚红的C0201 胰酶细胞消化液C0205 胰酶细胞消化液(不含EDTA)
4.对于EDTA可能会干扰后续的测试分析时,推荐选择不含EDTA的C0205 胰酶细胞消化液(不含EDTA)C0207 胰酶细胞消化液(含酚红,不含EDTA)
 

相关产品:
产品编号 产品名称 包装
C0201 胰酶细胞消化液 100ml
C0203 胰酶细胞消化液(含酚红) 100ml
C0205 胰酶细胞消化液(不含EDTA) 100ml
C0207 胰酶细胞消化液(含酚红,不含EDTA) 100ml
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