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试剂>

抗体>

凋亡与自噬>

Regulation of Apoptosis

Cytochrome C抗体(小鼠单抗)
产品编号: AC908
产品包装: 10μg
产品价格: 482.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
AC908 Cytochrome C抗体(小鼠单抗) 10μg 482.00元
来源 用途 交叉反应性 抗体识别位点 抗体类型 Cytochrome C分子量
Mouse IF,IHC,IP,F H,M,R Around residue 64 of rat Cytochrome C IgG1 15kD

      IF,Immunofluorescence;IHC,Immunohistochemistry;IP,Immunoprecipitation;F,Flow cytometry.
      H,human;M,mouse;R,Rat.
      本Cytochrome C抗体(Cytochrome C antibody)为进口分装,用大鼠Cytochrome C为抗原制备而成的抗Cytochrome C小鼠单克隆抗体。克隆号为6H2.B4。
      本Cytochrome C抗体可以用于免疫荧光、免疫组化、免疫沉淀和流式检测,但不适合用于Western检测。本抗体仅仅识别native Cytochrome C,不识别denatured Cytochrome C。
      本抗体可以用于通过免疫荧光或免疫组化检测线粒体内的Cytochrome C的释放情况,以确定Cytochrome C释放和凋亡的相关性,或直接用Cytochrome C释放来判断细胞凋亡的状况。正常细胞Cytochrome C的染色集中在线粒体,而凋亡细胞的
Cytochrome C则呈弥散分布。
      Cytochrome C,即细胞色素C,是线粒体内电子呼吸链中的一个重要蛋白。Cytochrome C在进化上高度保守。当细胞发生凋亡时,细胞色素C会从线粒体中释放到细胞浆中,并且作为细胞凋亡的关键调控步骤。在细胞色素C和dATP存在的情况下,Caspase-9和Apaf-1可以相互结合,并促使Caspase-9激活。细胞色素C的释放和Caspase-9的激活对于激活其它的Caspase包括Caspase-3,以及导致后续的DNA片段化(DNA fragmentation)至关重要。细胞凋亡的抑制蛋白,Bcl-2或Bcl-XL都可以抑制细胞色素C从线粒体的释放;而细胞凋亡的促进蛋白Bax,可以诱导细胞色素C从线粒体的释放。
      Cytochrome C从线粒体释放到细胞浆中常被作为细胞凋亡的一个重要指标。
      配套提供了免疫染色一抗稀释液,可以用于免疫染色时的一抗稀释。
      建议抗体使用时的稀释比例为(实际使用时需根据抗原水平的高低作适当调整):

IF IHC IP F
1:500 1:200 1:100 1:100

包装清单:

产品编号 产品名称 包装
AC908-1 Cytochrome C抗体(0.5μg/μl, 小鼠单抗) 10μg
AC908-2 免疫染色一抗稀释液 10ml
说明书 1份

保存条件:
      Cytochrome C抗体-20℃保存,免疫染色一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项: 
      由于本抗体识别的是native Cytochrome C,请尽量避免使用容易导致蛋白变性的条件处理细胞或组织。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.免疫染色:
可以使用随抗体提供的免疫染色一抗稀释液稀释抗体;如果不是滴染,使用后注意回收稀释好的一抗,具体操作可以参考如下网页: http://www.beyotime.com/support/immunol-staining.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品请点击如下按钮:

使用本产品的文献:
1. Gao LW, Zhang J, Yang WH, Wang B, Wang JW.
Glaucocalyxin A induces apoptosis in human leukemia HL-60 cells through mitochondria-mediateddeath pathway.
Toxicol In Vitro. 2011 Feb;25(1):51-63.
2. Muhammad Khan, Bin Zheng, Fei Yi, Azhar Rasul, Zhuyi Gu.
Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells.
Evid Based Complement Alternat Med. 2012;2012:957568.
3. Khan M, Yu B, Rasul A, Al Shawi A, Yi F, Yang H, Ma T.
Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2/M Phase Arrest.
Evid Based Complement Alternat Med. 2012;2012:703034.
4. Qian Y, Guan T, Huang M, Cao L, Li Y, Cheng H, Jin H, Yu D.
Neuroprotection by the soy isoflavone, genistein, via inhibition of mitochondria-dependentapoptosis pathways and reactive oxygen induced-NF-κB activation in a cerebral ischemia mousemodel.
Neurochem Int. 2012 Jun;60(8):759-67.
5. Khan M, Ding C, Rasul A, Yi F, Li T, Gao H, Gao R, Zhong L, Zhang K, Fang X, Ma T.
Isoalantolactone induces reactive oxygen species mediated apoptosis in pancreatic carcinomaPANC-1 cells.
Int J Biol Sci. 2012;8(4):533-47.
6. Gao Z, Kang X, Hu J, Ju Y, Xu C.
Induction of apoptosis with mitochondrial membrane depolarization bya glycyrrhetinic acidderivative in human leukemia K562 cells.
Cytotechnology. 2012 Aug;64(4):421-8.
7. Liang RR, Zhang S, Qi JA, Wang ZD, Li J, Liu PJ, Huang C, Le XF, Yang J, Li ZF.
Preferential inhibition of hepatocellular carcinoma by the flavonoid Baicalein through blocking MEK-ERKsignaling.
Int J Oncol. 2012 Sep;41(3):969-78.
8. Kang X, Hu J, Gao Z, Ju Y, Xu C.
Synthesis, anti-proliferative and proapoptotic activity of novel oleanolic acid azaheterocyclic derivatives.
Med. Chem. Commun. 2012;3:1245-9.
9. Gao S, Li H, Cai Y, Ye JT, Liu ZP, Lu J, Huang XY, Feng XJ, Gao H, Chen SR, Li M, Liu PQ.
Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis.
Arch Biochem Biophys. 2014 Jan 15;542:46-55.
10. Sun W, Zhang X, Ding X, Li H, Geng M, Xie Z, Wu H, Huang M.
Lactate dehydrogenase B is associated with the response to neoadjuvant chemotherapy in oral squamous cell carcinoma.
PLoS One. 2015 May 14;10(5):e0125976.
11. Zhang Y, Duan W, Owusu L, Wu D, Xin Y.
Epigallocatechin-3-gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non-cancerous liver cells.
Int J Mol Med. 2015 Jan;35(1):117-24.
12.Zhong Y, Jin C, Gan J, Wang X, Shi Z, Xia X, Peng X.
Apigenin attenuates patulin-induced apoptosis in HEK293 cells by modulating ROS-mediatedmitochondrial dysfunction and caspase signal pathway.
Toxicon. 2017 Oct;137:106-113.
13.Jiang H, Zhang C, Tang Y, Zhao J, Wang T, Liu H, Sun X.
The regulator of calcineurin 1 increases adenine nucleotide translocator 1 and leads to mitochondrial dysfunctions.
J Neurochem. 2017 Jan;140(2):307-319.
14.Chen L, Shi L, Wang W, Zhou Y.
ABCG2 downregulation in glioma stem cells enhances the therapeutic efficacy of demethoxycurcumin.
Oncotarget. 2017 Jun 27;8(26):43237-43247.
15.Xiao D, He H, Huang W, Oo TL, Wang A, He LF.
Analysis of Mitochondrial Markers of Programmed Cell Death.
Methods Mol Biol. 2018;1743:65-71.
16.Feng J, Luo L, Liu Y, Fu S, Chen J, Duan X, Xiang L, Zhang Y, Wu J, Fan J, Wen Q, Zhang Y, Yang J, Peng J, Zhao M, Yang L.
TP53-induced glycolysis and apoptosis regulator is indispensable for mitochondria qualitycontrol and degradation following damage.
Oncol Lett. 2018 Jan;15(1):155-160.
17.Dou C, Han M, Zhang B, Sun L, Jin X, Li T.
Chrysotoxene induces apoptosis of human hepatoblastoma HepG2 cells in vitro and in vivo viaactivation of the mitochondria-mediated apoptotic signaling pathway.
Oncol Lett. 2018 Apr;15(4):4611-4618.

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