Biotin/Cy3/FITC-labeled dUTP can replace dTTP as substrate for Taq DNA Polymerase (D7209)、phi29 DNA Polymerase (D7053)、Klenow Fragment, Exo- (D7039/D7041)、Klenow Fragment (D7037)、Terminal Deoxynucleotidyl Transferase (D7092/D7095), DNA Polymerase I,reverse transcriptase (D7188/D7176/D7160/D7153), etc. to label and detect DNA.
Biotin/Cy3/FITC-labeled UTP can replace UTP as substrate for RNA polymerases and modifying enzymes such as T3/T7/SP6 RNA polymerase, thereby labeling RNA for RNA detection.
Selection Guide for Beyotime Biotin/Cy3/FITC-labeled dUTP & UTP
Cat. No. | Product Name | Target | Applications |
D7331 | Biotin-11-dUTP (1mM, Nuclease free) | DNA | Biotin labeling of DNA. Biotin-labeled DNA probes can replace radiolabeled DNA probes in in situ hybridization, chromosome localization, etc., and can be detected using fluorescently labeled streptavidin, streptavidin-coupled HRP or alkaline phosphatase, or streptavidin-coupled antibodies. |
D7332 | Cy3-dUTP (1mM, Nuclease free) | DNA | Red fluorescent labeling of DNA. For example, generating Cy3-labeled DNA red fluorescent probe by PCR. After gel electrophoresis, the fluorescence of Cy3-labeled DNA is clearly visible after excitation, and no further staining is required. The fluorescent labeled probes generated by this method are suitable for FISH, microarrays, blot, multicolor fluorescence analysis, etc. The excitation/emission wavelengths are 550/570nm. |
D7333 | Fluorescein-12-dUTP (1mM, Nuclease free) | DNA | Green fluorescent labeling of DNA. For example, generating Fluorescein-labeled DNA green fluorescent probe by PCR. After gel electrophoresis, the fluorescence of Fluorescein-labeled DNA is clearly visible after excitation, and no further staining is required. The fluorescent labeled probes generated by this method are suitable for FISH, microarrays, blot, multicolor fluorescence analysis, etc. The excitation/emission wavelengths are 495/521nm. |
D7336 | Biotin-16-UTP (10mM, Nuclease free) | RNA | Biotin labeling of RNA. Biotin-labeled RNA probes can replace radiolabeled RNA probes in in situ hybridization, chromosome localization, etc., and can be detected using fluorescently labeled streptavidin, streptavidin-coupled HRP or alkaline phosphatase, or streptavidin-coupled antibodies. |
D7337 | Cy3-UTP (10mM, Nuclease free) | RNA | Red fluorescent labeling of RNA. For example, generating Cy3-labeled RNA red fluorescent probe by PCR. After gel electrophoresis, the fluorescence of Cy3-labeled RNA is clearly visible after excitation, and no further staining is required. The fluorescent labeled probes generated by this method are suitable for FISH, microarrays, blot, multicolor fluorescence analysis, etc. Cy3-UTP can also be doped into intracellularly synthesized RNA, thereby labeling newly synthesized RNA in the cell. The excitation/emission wavelengths are 550/570nm. |
D7338 | Fluorescein-12-UTP (10mM, Nuclease free) | RNA | Green fluorescent labeling of RNA. For example, generating Fluorescein-labeled RNA green fluorescent probe by PCR. After gel electrophoresis, the fluorescence of Fluorescein-labeled RNA is clearly visible after excitation, and no further staining is required. The fluorescent labeled probes generated by this method are suitable for FISH, microarrays, blot, multicolor fluorescence analysis, etc. Fluorescein-12-UTP can also be doped into intracellularly synthesized RNA, thereby labeling newly synthesized RNA in the cell. The excitation/emission wavelengths are 495/521nm, respectively. |
Product Information:
Cat. No. | Product Name | Pack Size | Price |
D7331-20μl | Biotin-11-dUTP (1mM, Nuclease free) | 20μl | |
D7331-100μl | Biotin-11-dUTP (1mM, Nuclease free) | 100μl | |
D7331-500μl | Biotin-11-dUTP (1mM, Nuclease free) | 500μl | |
D7332-20μl | Cy3-dUTP (1mM, Nuclease free) | 20μl | |
D7332-100μl | Cy3-dUTP (1mM, Nuclease free) | 100μl | |
D7332-500μl | Cy3-dUTP (1mM, Nuclease free) | 500μl | |
D7333-20μl | Fluorescein-12-dUTP (1mM, Nuclease free) | 20μl | |
D7333-100μl | Fluorescein-12-dUTP (1mM, Nuclease free) | 100μl | |
D7333-500μl | Fluorescein-12-dUTP (1mM, Nuclease free) | 500μl | |
D7336-20μl | Biotin-16-UTP (10mM, Nuclease free) | 20μl | |
D7336-100μl | Biotin-16-UTP (10mM, Nuclease free) | 100μl | |
D7336-500μl | Biotin-16-UTP (10mM, Nuclease free) | 500μl | |
D7337-20μl | Cy3-UTP (10mM, Nuclease free) | 20μl | |
D7337-100μl | Cy3-UTP (10mM, Nuclease free) | 100μl | |
D7337-500μl | Cy3-UTP (10mM, Nuclease free) | 500μl | |
D7338-20μl | Fluorescein-12-UTP (10mM, Nuclease free) | 20μl | |
D7338-100μl | Fluorescein-12-UTP (10mM, Nuclease free) | 100μl | |
D7338-500μl | Fluorescein-12-UTP (10mM, Nuclease free) | 500μl |