使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测:
A. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
B. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C. 回收稀释的一抗,4℃保存以备下次继续使用。
D. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:http://www.beyotime.com/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:
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Fig. 1. Western blot analysis of ERK2 on different cell lysates using anti- ERK2 antibody at 1/2000 dilution. Positive control: Lane 1: Jurkat Lane 2:A431 Lane 3: PC12 Lane 4: NIH/3T3 Lane 5: Hela Lane 6: HT-29 Lane 7: MCF-7. | Fig. 2. Immunocytochemistry staining of ERK2 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. |
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Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ERK2 antibody. Counter stained with hematoxylin. | Fig. 4 Flow cytometric analysis of Hela cells with ERK2.antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).Goat anti rabbit IgG (FITC) was used as the secondary antibody. |