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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P7928-100μg | Recombinant Wnt Surrogate Fc Chimera (Organoid Culture Grade) | 100μg | 2132.00元 |
| P7928-1mg | Recombinant Wnt Surrogate Fc Chimera (Organoid Culture Grade) | 1mg | 10660.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | - | - | HEK293 | confidential | 59.2kDa | C-Fc Tag |
| About this protein | |
| Name | Recombinant Wnt Surrogate Fc Chimera; 重组Wnt3a Surrogate蛋白(Fc) |
| Synonyms | - |
| Accession | Wnt Surrogate (confidential) - EFGGGS - IgG1 (Pro100-Lys330) |
| Purity | >90%, by SDS-PAGE under reducing conditions. |
| Biological Activity | Measured by its ability to induce Topflash reporter activity in HEK293 reporter cells. The ED50 for this effect is 10-50ng/ml. |
| Physical Appearance | Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from PBS-3% Trehalose Buffer. |
| Endotoxin | <0.10EU/μg of the protein by the LAL method. |
| Reconstitution | Initially reconstitute in sterile distilled water to 0.1-1.0mg/ml. |
| Category | Cytokine |
| Background | Wnt-3a, a member of the Wingless-type MMTV integration site (Wnt) family, plays a crucial role in various developmental processes. Wnt proteins, including Wnt-3a, bind to Frizzled family receptors on the cell surface, along with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6), leading to the stabilization of intracellular beta-catenin levels. Increased intracellular beta-catenin levels result in the binding of beta-catenin to TCF/LEF transcription factors, which in turn induces the expression of Wnt target genes. Wnt-3a is a hydrophobic glycoprotein with a molecular weight of 44kDa and contains a conserved pattern of 24 cysteine residues. It possesses two N-linked glycosylation sites (Asn 87, Asn 298), and the modification of Ser 209 with palmitoleic acid is crucial for its biological activity. Glycosylation and acylation are essential for efficient Wnt secretion and biological function, respectively. Human Wnt-3a shares a high degree of amino acid sequence identity (96%) with its counterparts in mice, bovines, and canines, as well as 89%, 86%, and 84% identity with chicken, Xenopus, and zebrafish Wnt-3a, respectively. It also shares 87% amino acid identity with Wnt3. Wnt-3a also plays a significant role in organoid culture systems. Organoids are three-dimensional structures derived from stem cells that mimic the architecture and functionality of specific organs. In this context, Wnt-3a has been shown to have diverse effects on the development and maintenance of various types of organoids. The addition of Wnt-3a to intestinal organoid cultures enhances their self-renewal and promotes the formation of crypt-like structures, which are essential for intestinal epithelial cell homeostasis. It has also been demonstrated that Wnt-3a supplementation promotes the proliferation and differentiation of hepatic progenitor cells, contributing to the generation and expansion of liver organoids. Furthermore, in brain organoids, Wnt-3a is crucial for the proper development of the hippocampus, a region involved in learning and memory. Its presence during the early stages of brain organoid formation leads to the generation of well-organized hippocampal structures. Wnt-3a signaling has also been implicated in the patterning and regionalization of other brain regions within organoids, such as the cerebral cortex and midbrain. Additionally, Wnt-3a has been found to play a role in promoting the self-renewal and differentiation of neural progenitor cells within neural organoids. This signaling pathway is involved in regulating the balance between neural stem cell proliferation and differentiation, thereby influencing the development and maturation of neural tissue within organoids. Overall, the incorporation of Wnt-3a in organoid culture systems provides a valuable tool for directing and modulating the development, self-renewal, and differentiation of various types of organoids, enabling researchers to study organogenesis, disease modeling, and drug screening. |
| 产品编号 | 产品名称 | 包装 |
| P7928-100μg | Recombinant Wnt Surrogate Fc Chimera (Organoid Culture Grade) | 100μg |
| P7928-1mg | Recombinant Wnt Surrogate Fc Chimera (Organoid Culture Grade) | 1mg |
| — | 说明书 | 1份 |
-20ºC或更低温度保存，至少一年有效。
注意事项：由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后，须分装后-20ºC或更低温度冻存，以避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
图1.碧云天Recombinant Wnt Surrogate Fc Chimera (Organoid Culture Grade) (P7928)检测效果图。左图：The purity of the recombinant Wnt surrogate Fc chimera protein was analyzed using SDS-PAGE under reducing conditions and stained using Coomassie blue. 右图：Measured by its ability to induce Topflash reporter activity in HEK293 reporter cells. The luciferase expression was analyzed using the Firefly Luciferase Reporter Gene Assay Kit (RG005/RG006). The ED50 for this effect is 10-50ng/ml. 本图仅供参考。| 产品编号 | 产品名称 | 包装 |
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