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多肽与蛋白> 蛋白检测> Western检测
QuickBlock™ Western一抗稀释液
产品编号: P0256-100ml
产品包装:100ml
选择包装
价格: ¥ 123.00
产品简介
使用说明
产品文件
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产品编号 产品名称 产品包装 产品价格
P0256FT QuickBlock™ Western一抗稀释液(试用装) 15ml .00元
P0256-100ml QuickBlock™ Western一抗稀释液 100ml 123.00元
P0256-500ml QuickBlock™ Western一抗稀释液 500ml 498.00元

碧云天生产的QuickBlock™ Western一抗稀释液(QuickBlock™ Primary Antibody Dilution Buffer for Western Blot)是最新一代的高效一抗稀释液,总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉等的一抗稀释液,可以用于Western blot (WB)时一抗的稀释和配制。
QuickBlock™ Western一抗稀释液可以有效节约一抗。本产品稀释和配制的一抗可以在4℃保存和使用不少于1个月,并且可以反复多次用于Western,利于节约一抗。
QuickBlock™ Western一抗稀释液稀释的一抗孵育后背景极低。本稀释液不含血清和白蛋白,确保极高的信噪比。
QuickBlock™ Western一抗稀释液兼容性好。后续兼容辣根过氧化物酶(Horseradish peroxidase, HRP)、碱性磷酸酶(Alkalinephosphatase, AP)和生物素标记的二抗。本产品不含生物素,不会干扰基于生物素的检测。
QuickBlock™ Western一抗稀释液使用便捷。本产品无需添加任何额外的试剂,可以直接用于一抗的稀释。
按照每次一抗稀释需要10ml一抗稀释液计算,一个100ml包装的QuickBlock™ Western一抗稀释液可以稀释10次一抗,一个500ml包装的QuickBlock™ Western一抗稀释液可以稀释50次一抗。
包装清单:

产品编号 产品名称 包装
P0256-100ml QuickBlock™ Western一抗稀释液 100ml
P0256-500ml QuickBlock™ Western一抗稀释液 500ml
说明书 1份

保存条件:
4℃保存,一年有效。长期不使用可以-20℃保存。
注意事项:
取放PVDF膜和NC膜应使用平头镊子,并仅轻轻夹取其边角,操作过程须避免膜表面产生划痕、折痕或压痕等痕迹。
PVDF膜一经浸润和活化,需一直保持湿润,根据Western进行到的具体步骤可放置于western转膜液或洗涤液等适当溶液中,否则可能会产生难以封闭的异常背景。
为进一步提高信噪比,推荐同时使用QuickBlock™ Western封闭液(P0252)QuickBlock™Western二抗稀释液(P0258)用于封闭及二抗的稀释。
为了能使稀释的一抗可以反复多次使用,一抗孵育结束后稀释的一抗应立即存放在4℃,以便于后续重复使用。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 参考所用一抗的说明,以及样品中目的蛋白的含量,按照适当比例例如1:1000、1:500等稀释一抗。一抗稀释后即可直接用于Western。一次Western结束后,可以回收稀释的一抗,4℃保存,以用于下次的Western。
2. 详细的Western操作可以参考如下的相关网页:http://www.beyotime.com/support/western.htm

相关产品:

产品编号 产品名称 包装
P0023A-100ml Western一抗稀释液 100ml
P0023A-500ml Western一抗稀释液 500ml
P0023B-100ml Western封闭液 100ml
P0023B-500ml Western封闭液 500ml
P0023D-100ml Western二抗稀释液 100ml
P0023D-500ml Western二抗稀释液 500ml
P0220 QuickBlock™封闭液(PBS) 100ml
P0222 QuickBlock™封闭液(PBSTw)  100ml
P0226 QuickBlock™封闭液(PBSTx) 100ml
P0228 QuickBlock™封闭液(TBS) 100ml
P0231 QuickBlock™封闭液(TBSTw) 100ml
P0233 QuickBlock™封闭液(TBSTx)  100ml
P0235 QuickBlock™封闭液(10X)  100ml
P0252-100ml QuickBlock™ Western封闭液 100ml
P0252-500ml QuickBlock™ Western封闭液 500ml
P0256-100ml QuickBlock™ Western一抗稀释液 100ml
P0256-500ml QuickBlock™ Western一抗稀释液 500ml
P0258-100ml QuickBlock™ Western二抗稀释液 100ml
P0258-500ml QuickBlock™ Western二抗稀释液 500ml


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