Western封闭液(P0023B-100ml)

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Western封闭液

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产品编号: P0023B-100ml

产品包装:100ml

选择包装

100ml 500ml

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量

瓶

说明书下载

价格:¥118.00元

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产品编号	产品名称	产品包装	产品价格
P0023B-100ml	Western封闭液	100ml	118.00元
P0023B-500ml	Western封闭液	500ml	486.00元

碧云天生产的Western封闭液(Blocking Buffer)可以用于Western时转移了蛋白样品的PVDF膜或NC膜的封闭。封闭后，可以减小后续的一抗或二抗和膜的非特异性结合，降低背景，增强信噪比。
本产品的主要有效成分为经过反复优化的适量BSA和适当的去垢剂等，一方面能有效封闭膜上的蛋白结合位点，另一方面能有效减少后续一抗和二抗的非特异性结合。
本产品可以用于一抗的稀释，但更推荐使用Western检测时一抗稀释专用的Western一抗稀释液(P0023A)或QuickBlock™ Western封闭液(P0252)。
关于不同封闭液的比较和选择，请参考碧云天的相关网页：http://www.beyotime.com/support/blocking-buffer.htm。
按照每张膜封闭需要5-10毫升封闭液计算，一个100ml包装的Western封闭液可以封闭10-20张膜，一个100ml包装的Western封闭液可以封闭50-100张膜。
包装清单：

产品编号	产品名称	包装
P0023B-100ml	Western封闭液	100ml
P0023B-500ml	Western封闭液	500ml
—	说明书	1份

保存条件：
4℃保存，一年有效。长期不使用可以-20℃保存。
注意事项：
通常在室温封闭60分钟即可。如果对于一些背景非常高的抗体，可以4℃封闭过夜。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 完成转膜后，用Western洗涤液洗涤蛋白膜1-2分钟。加入Western封闭液，封闭60分钟，然后进行一抗孵育等后续操作。
2. 详细的Western操作可以参考我们的相关网页：http://www.beyotime.com/support/western.htm。

相关产品：

产品编号	产品名称	包装
P0023A-100ml	Western一抗稀释液	100ml
P0023A-500ml	Western一抗稀释液	500ml
P0023B-100ml	Western封闭液	100ml
P0023B-500ml	Western封闭液	500ml
P0023D-100ml	Western二抗稀释液	100ml
P0023D-500ml	Western二抗稀释液	500ml
P0220	QuickBlock™封闭液(PBS)	100ml
P0222	QuickBlock™封闭液(PBSTw)	100ml
P0226	QuickBlock™封闭液(PBSTx)	100ml
P0228	QuickBlock™封闭液(TBS)	100ml
P0231	QuickBlock™封闭液(TBSTw)	100ml
P0233	QuickBlock™封闭液(TBSTx)	100ml
P0235	QuickBlock™封闭液(10X)	100ml
P0252-100ml	QuickBlock™ Western封闭液	100ml
P0252-500ml	QuickBlock™ Western封闭液	500ml
P0256-100ml	QuickBlock™ Western一抗稀释液	100ml
P0256-500ml	QuickBlock™ Western一抗稀释液	500ml
P0258-100ml	QuickBlock™ Western二抗稀释液	100ml
P0258-500ml	QuickBlock™ Western二抗稀释液	500ml

相关产品请点击如下按钮：

Western检测

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miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2.

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LncRNA-135528 inhibits tumor progression by up-regulating CXCL10 through the JAK/STAT pathway.

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Electroacupuncture inhibits sodium nitroprusside‑mediated chondrocyte apoptosis through the mitochondrial pathway.

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Astaxanthin ameliorates experimental diabetes-induced renal oxidative stress and fibronectin by upregulating connexin43 in glomerular mesangial cells and diabetic mice.

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