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DNA提取与纯化>

DNA纯化胶回收

PCR纯化试剂盒/DNA纯化试剂盒
产品编号: D0033
产品包装: 50次
产品价格: 142.00元
产品简介
使用说明
产品图片
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产品编号 产品名称 产品包装 产品价格
D0033 PCR纯化试剂盒/DNA纯化试剂盒 50次 142.00元

      碧云天的PCR纯化试剂盒(PCR Clean Up Kit),也称DNA纯化试剂盒(DNA Purification Kit)是一种用于PCR产物纯化或从多种DNA反应体系中纯化DNA的试剂盒。
      适用于PCR反应后去除引物、酶、矿物油、甘油、盐等杂质;也同样适用于酶切、连接、磷酸化、补平或切平、随机引物等反应后的DNA纯化。纯化所得DNA可直接用于酶切、连接、转化细菌、测序、PCR、杂交等后续操作。本试剂盒适用于纯化100bp-10kb DNA,长至30个碱基的引物均可被完全去除。
      本试剂盒采用了一种新型的DNA纯化柱。在特定条件下,使DNA能在离心过柱的瞬间,结合到DNA纯化柱上,在一定条件下又能将DNA充分洗脱,从而实现DNA的快速纯化。无需酚氯仿抽提,无需酒精沉淀,12个样品只需不足15分钟即可完成。 
      每个DNA纯化柱可以结合的DNA量的上限约为15微克。
      DNA回收效率约为90%。接近100bp或10kb的DNA片段回收效率要略低一些,大于10kb的DNA回收效率迅速下降。另外如果样品中DNA含量特别低也会导致回收效率下降。本试剂盒的纯化效果参见图1。


图1. 本试剂盒纯化效果图。本图仅作参考,不同的样品不同的纯化条件,实际的纯化效果可能和上图有一定的差别。

       本试剂盒足够纯化50个平均体积不超过400微升的DNA样品。
包装清单:

产品编号 产品名称 包装
D0033-1 溶液I (DNA纯化结合液) 20ml
D0033-2 溶液II (洗涤液) 26ml (第一次使用前加入39ml无水乙醇)
D0033-3 溶液III (洗脱液) 3ml
D0033-4 DNA纯化柱及废液收集管 50套
说明书 1份

保存条件:
      室温保存,一年有效。
注意事项:
      第一次使用前在溶液II(洗涤液)中加入39ml无水乙醇,混匀,并在瓶上做好标记。
      溶液I对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
      本试剂盒所有操作均在室温进行,操作时无需冰浴。所有离心也均在室温进行。
      废液收集管在一次抽提中需多次使用,切勿中途丢弃。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. 加入等体积的溶液I,混匀。
例如DNA样品体积为100微升,则加100微升溶液I。如果等体积混合后体积较大,可以把部分样品加入到纯化柱内,经离心处理后,再加入剩余的样品继续处理。矿物油对于本试剂盒没有干扰。
2. 把与溶液I等体积混匀的样品加入到DNA纯化柱内,室温放置1分钟。通常室温放置数秒待溶液浸润到纯化柱中即可。
3. 最高速(16,000g,约12,000-14,000rmp左右)离心1分钟,倒弃收集管内的液体。
注意:这一步尽量要达到16,000g,较低离心速度会导致回收效率下降。
4. 在DNA纯化柱内加入700微升溶液II,室温放置1分钟
5. 最高速离心1分钟,洗去杂质。倒弃收集管内的液体。
6. 再加入500微升溶液II,最高速离心1分钟,进一步洗去杂质。倒弃收集管内的液体。
7. 最高速再离心1分钟,除去残留液体并让残留的乙醇充分挥发。
8. 将DNA纯化柱置于1.5毫升离心管上,加入50微升溶液III至管内柱面上,放置1分钟
1.5ml离心管作为收集管。溶液III需要直接加至管内柱面中央,使液体被纯化柱吸收。如果不慎将溶III沾在管壁上,一定要震动管子,使液体滑落到管底,以便被纯化柱吸收。也可以用重蒸水或Milli-Q级纯水替代溶液III,但是水的pH值应不小于6.5。如需得到较高浓度的DNA,可以只加20-30微升溶液III,但产量会略有下降。放置较长时间例如3-5分钟,会对提高产量略有帮助。
9. 最高速离心1分钟,所得液体即为高纯度DNA
相关产品请点击如下按钮:

使用本产品的文献:
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Wnt1 positively regulates CD36 expression via TCF4 and PPAR-γ in macrophages.
Cell Physiol Biochem. 2015 Mar;35(4):1289-302.
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Hydrogen Sulfide Alleviates Cadmium-Induced Cell Death through Restraining ROS Accumulation in Roots of Brassica rapa L. ssp. pekinensis.
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Biochem Pharmacol. 2016 Jul 15;112:37-49.
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The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation ofUBXN1 in EGFRwt/vIII glioma cells.
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Antiviral Res. 2016 Oct;134:97-107.
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Mol Med Rep. 2017 Jul;16(1):710-718.
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ASAP1 gene polymorphisms are associated with susceptibility to tuberculosis in a ChineseXinjiang Muslim population.
Exp Ther Med. 2018 Apr;15(4):3392-3398.
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Genetic diversities of MT-ND1 and MT-ND2 genes are associated with high-altitude adaptation in yak.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Apr;29(3):485-494.
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MAT2A promotes porcine adipogenesis by mediating H3K27me3 at Wnt10b locus and repressingWnt/β-catenin
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LncRNA MALAT1 regulates oxLDL-induced CD36 expression via activating β-catenin.
Biochem Biophys Res Commun. 2018 Jan 15;495(3):2111-2117.
34.Zhang Y, Guan Q, Liu Y, Zhang Y, Chen Y, Chen J, Liu Y, Su Z.
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