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 BCIP/NBT碱性磷酸酯酶显色试剂盒

  产品编号C3206
  产品包装: 共100ml
  产品价格: 215.00元
产品编号 产品名称 产品包装 产品价格
C3206 BCIP/NBT碱性磷酸酯酶显色试剂盒 共100ml 215.00元

      BCIP/NBT碱性磷酸酯酶显色试剂盒(BCIP/NBT Alkaline Phosphatase Color Development Kit)是一种用于免疫组化显
色、Western等膜显色和诱导多功能干细胞iPS鉴定等的试剂盒。
      碱性磷酸酯酶(Alkaline Phosphatase, AP/ALP/AKP/ALKP/ALPase/Alk Phos)常被称作碱性磷酸酶(EC 3.1.3.1),是一类水解酶,通过水解磷酸单酯将底物分子上的磷酸基团除去,并生成磷酸根离子和自由的羟基,其去磷酸化作用的底物包括核苷酸、蛋白质和生物碱等,并在碱性条件下最为有效。该酶是一组同功酶的统称。常见的小牛肠碱性磷酸酶(Calf Intestinal Alkaline
Phosphatase, CIAP/CIP)被广泛用于二抗等的标记最终用于蛋白和核酸等的检测,也常用于DNA或RNA 5’和3’末端的去磷酸
化(去单磷酸化),特别是质粒的5’末端去磷酸化以避免质粒自连等。
      BCIP/NBT是碱性磷酸酯酶的常用底物。在碱性磷酸酯酶的催化下,BCIP会被水解产生强反应性的产物,该产物会和NBT发生反应,形成不溶性的深蓝色至蓝紫色的NBT-formazan。
      本试剂盒可以用于细胞或组织的碱性磷酸酯酶显色包括诱导多功能干细胞iPS的鉴定,也可以用于Western等结合有碱性磷酸酯酶的膜的显色检测。同时也可以用于细胞或组织内源性的碱性磷酸酯酶显色。
      有Nature论文注明使用了本试剂盒,参考使用本产品的文献2。
包装清单:

产品编号 产品名称 包装
C3206-1 碱性磷酸酯酶显色缓冲液 100ml
C3206-2 BCIP溶液(300X) 350μl
C3206-3 NBT溶液(150X) 700μl
说明书 1份

保存条件:
      4℃保存,一年有效。BCIP溶液和NBT溶液需避光保存。
注意事项:
      BCIP对人体有刺激性,NBT对人体有害,请注意适当防护。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 对于组织切片或细胞样品或膜,在与碱性磷酸酯酶标记的抗体或其它形式的探针孵育后,用适当洗涤液洗涤3-5次,每次3-5分钟。对于检测内源性碱性磷酸酯酶的组织或细胞样品,在适当固定后,也用适当洗涤液洗涤3-5次,每次3-5分钟。
2. 按照如下比例依次加入各溶液,混匀后即配制成BCIP/NBT染色工作液:

碱性磷酸酯酶显色缓冲液 3ml 10ml
BCIP溶液(300X) 10μl 33μl
NBT溶液(150X) 20μl 66μl
BCIP/NBT染色工作液 3.03ml 10.1ml

3. 最后一次洗涤完毕后,去除洗涤液,加入适量BCIP/NBT染色工作液,确保能充分覆盖样品。
4. 室温避光孵育5-30分钟或更长时间(可长达24小时),直至显色至预期深浅。
5. 去除BCIP/NBT染色工作液,用蒸馏水洗涤1-2次即可终止显色反应。
6. 对于组织切片或细胞样品,显色反应终止后,如有必要可以用中性红染色液(neutral red staining solution)染色,以便于观察。对于膜,显色反应终止后,可以室温晾干避光保存。

相关产品请点击如下按钮:

使用本产品的文献:
1. Jiang S, Zu Y, Fu Y, Zhang Y, Efferth T.
Activation of the mitochondria-driven pathway of apoptosis in human PC-3 prostate cancer cells by a novel hydrophilic paclitaxel derivative, 7-xylosyl-10-deacetylpaclitaxel.
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iPS cells produce viable mice through tetraploid complementation.
Nature. 2009 Sep 3;461(7260):86-90.
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A new bone repair scaffold combined with chitosan/hydroxyapatite and sustained releasing icariin.
Chinese Sci Bull. 2009;54:2953-61.Z
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Treatment of transplanted tumor of lung adenocarcinoma A549 transfected by human somatostatinreceptor subtype 2 (hsstr2) gene with 188Re-RC-160.
Nucl Med Biol. 2010 Nov;37(8):977-87.
5. Luo M, Liu X, Zu Y, Fu Y, Zhang S, Yao L, Efferth T.
Cajanol, a novel anticancer agent from Pigeonpea [Cajanus cajan (L.) Millsp.] roots,induces apoptosis in human breast cancer cells through a ROS-mediated mitochondrialpathway.
Chem Biol Interact.2010;188(1):151-60.  
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The regulation of tendon stem cell differentiation by the alignment of nanofibers.
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8.Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Wang X, Wang L, Zeng F, Zhou Q
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Progesterone modulates the proliferation and differentiation of human periodontalligament cells.
Calcif Tissue Int. 2010 Aug;87(2):158-67.
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11.Liu H, Yang Y, Xiao J, Lv Y, Liu Y, Yang H, Zhao L.
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Anat Rec (Hoboken). 2010 Nov;293(11):1838-46.
12.Ji YB, Qu ZY, Zou X.
Juglone-induced apoptosis in human gastric cancer SGC-7901 cells via the mitochondrialpathway.
Exp Toxicol Pathol. 2011 Jan;63(1-2):69-78.
13.You L, Li L, Xu Q, Ren J, Zhang F.
Postconditioning reduces infarct size and cardiac myocyte apoptosis via the opioid receptor and JAK-STAT signaling pathway.
Mol Biol Rep.2011 Jan;38(1):437-43.
14.Liu D, Zhang R, Yang X, Xu Y, Tang Z, Tian W, Shen Q.
Expression, purification and characterization of two thermostable endoglucanases cloned from alignocellulosic decomposing fungi Aspergillus fumigatus Z5 isolated from compost.
Protein Expr Purif. 2011 Oct;79(2):176-86.
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Advanced glycation end-products induce heparanase expression in endothelial cells by thereceptor for advanced glycation end products and through activation of the FOXO4 transcription factor.
Mol Cell Biochem. 2011 Aug;354(1-2):47-55.
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Protective effect of prostaglandin E1 on renal microvascular injury in rats of acute aristolochic acidnephropathy.
Ren Fail. 2011;33(2):225-32.
17.Gu J, Sun X, Wang G, Li M, Chi M.
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18.Yu H, Zhang T, Cai L, Qu Y, Hu S, Dong G, Guan R, Xu X, Xing L.
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Molecules. 2011 Sep 27;16(10):8165-80
19.Wang Y, Zhao Y, Liu Y, Tian L, Jin D.
Chamaejasmine inactivates Akt to trigger apoptosis in human HEp-2 larynx carcinomacells.
Molecules. 2011 Sep 27;16(10):8152-64.
20.Fang W, Liu S, Nie Y.
Anticancer activity of chamaejasmine: effect on tubulin protein.
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21.Jiang S, Zu Y, Wang Z, Zhang Y, Fu Y.
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Planta Med. 2011 Jul;77(10):1005-12.
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Molecules. 2011 Sep 28;16(10):8228-42.
23.Chen M, Wang X, Ye Z, Zhang Y, Zhou Y, Tan WS.
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Biomaterials. 2011 Oct;32(30):7532-42.
24.Li Q, Qiu W, Zhu Q, Zu Y, Deng X, Zhao T, Jiang C, Zhang L.
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Molecules. 2011 Sep 13;16(9):7803-14.
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Biol Reprod.2012 Mar 22;86(3):83.
27.Han D, Liu XY, Jiao GZ, Liang B, He N, Gao WQ, Tan JH.
Cyclin B1 turnover and the mechanism causing insensitivity of fully grown mouse oocytes tocycloheximide inhibition of meiotic resumption.
Theriogenology. 2012 Jun;77(9):1900-10.
28.Liu D, Zhang R, Yang X, Zhang Z, Song S, Miao Y, Shen Q.
Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33.
Microb Cell Fact. 2012 Feb 17;11:25.
29.Sun D, Ma Y, Han H, Yin Z, Liu C, Feng J, Zhou X, Li X, Xiao A, Yu R.
Thrombospondin-1 short hairpin RNA suppresses tubulointerstitial fibrosis in the kidneyof ureteralobstruction by ameliorating peritubular capillary injury.
Kidney Blood Press Res. 2012;35(1):35-47.
30.Zhao L, Liu L, Wu Z, Zhang Y, Chu PK.
Effects of micropitted/nanotubular titania topographies on bone mesenchymal stem cellosteogenic differentiation.
Biomaterials. 2012 Mar;33(9):2629-41.
31.Mao SS, Hua R, Zhao XP, Qin X, Sun ZQ, Zhang Y, Wu YQ, Jia MX, Cao JL, Zhang YM.
Exogenous Administration of PACAP Alleviates Traumatic Brain Injury in Rats through a Mechanism Involving the TLR4/MyD88/NF-κB Pathway.
J Neurotrauma. 2012 Jul 1;29(10):1941-59.
32.Zhao X, Liu L, Wang FK, Zhao DP, Dai XM, Han XS.
Coculture of vascular endothelial cells and adipose-derived stem cells as a source for boneengineering.
Ann Plast Surg. 2012 Jul;69(1):91-8.
33.Gu S, He J.
Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells.
Molecules. 2012 Jan 9;17(1):598-612.
34.Zhang L, Zhang L, Han X, Du M, Zhang Y, Feng Z, Yi H, Zhang Y.
Enhancement of transglutaminase production in Streptomyces mobaraensis as achieved bytreatment with excessive MgCl2.
Appl Microbiol Biotechnol. 2012 Mar;93(6):2335-43.
35.Gao Q, Zhao L, Song Z, Yang G.
Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs.
Mol Biol Rep. 2012 May;39(5):5791-804.
36.Wen L, Wang Y, Wang H, Kong L, Zhang L, Chen X, Ding Y.
L-type calcium channels play a crucial role in the proliferation and osteogenic differentiation ofbone marrow mesenchymal stem cells.
Biochem Biophys Res Commun. 2012 Aug 3;424(3):439-45.
37.Tang RX, Kong FY, Fan BF, Liu XM, You HJ, Zhang P, Zheng KY.
HBx activates FasL and mediates HepG2 cell apoptosis through MLK3-MKK7-JNKs signal module.
World J Gastroenterol. 2012 Apr 7;18(13):1485-95.
38.Liu Y, Xiao Z, Yang D, Ren L, Liu G, Yang L.
Effects of the cyclin-dependent kinase 10 (CDK10) on the tamoxifen sensitivity of keloid samples.
Molecules. 2012 Feb 1;17(2):1307-18.
39.Na L.
Growth Inhibition and Cell Cycle Arrest of Kappa-Selenocarrageenan and Paclitaxel on HepG2 Cells.
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40.Luan HF, Zhao ZB, Zhao QH, Zhu P, Xiu MY.
Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and reperfusioninjury mediated by the JAK2/STAT3 survival pathway.
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41.Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P.
The anti-inflammatory mechanism of heme oxygenase-1 induced by hemin in primary rat alveolarmacrophages.
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42.Qin X, Sun ZQ, Dai XJ, Mao SS, Zhang JL, Jia MX, Zhang YM.
Toll-like receptor 4 signaling is involved in PACAP-induced neuroprotection in BV2 microglial cellsunder OGD/reoxygenation.
Neurol Res. 2012 May;34(4):379-89.
43.Jiao GZ, Cao XY, Cui W, Lian HY, Miao YL, Wu XF, Han D, Tan JH.
Developmental potential of prepubertal mouse oocytes is compromised due mainly to theirimpaired synthesis ofglutathione.
PLoS One. 2013;8(3):e58018.
44.Chuan J, Li Y, Yang L, Sun X, Zhang Q, Gong T,Zhang Z.
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45.Li N, Liu N, Zhou J, Tang L, Ding B, Duan Y, Jin Y.
Inflammatory environment induces gingival tissue-specific mesenchymal stem cells to differentiate towards a pro-fibrotic phenotype.
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46.Cai C, Yuan GJ, Huang Y, Yang N, Chen X, Wen L, Wang X, Zhang L, Ding Y.
Estrogen-related receptorαis involved in the osteogenic differentiation of mesenchymal stem cells isolated fromhuman periodontal ligaments.
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Cajaninstilbene acid (CSA) exerts cytoprotective effects against oxidative stressthrough the Nrf2-dependent antioxidant pathway.
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Protection by salidroside against bone loss via inhibition of oxidative stress andbone-resorbing mediators.
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50.Li H, Zhang L, Cui Y, Luo X, Xue C, Wang S.
Expression of soluble recombinant transglutaminase from Zea mays in Pichia pastoris.
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51.Sun Y, Mu F, Li C, Wang W, Luo M, Fu Y, Zu Y.
Aspidin BB, a phloroglucinol derivative, induces cell cycle arrest and apoptosis inhuman ovarian HO-8910 cells.
Chem Biol Interact.  2013 Jul 5;204(2):88-97.
52.Dong YH, Li ZQ, Sun Y, Zhuang L, Wang YK, Sun Q.
Downregulation of pigment epithelium-derived factor in condyloma acuminatum.
J Int Med Res. 2013 Apr;41(2):365-70.
53.Yang Z, Liu J, Liu H, Qiu M, Liu Q, Zheng L, Pang M, Quan F, Zhang Y.
Isolation and Characterization of SSEA3(+) Stem Cells Derived from Goat SkinFibroblasts.
Cell Reprogram.2013 Jun;15(3):195-205.
54.Liang L, Gao C, Luo M, Zhao C, Wang W, Gu C, Yu J, Fu Y.
The Phytoestrogenic Compound Cajanol from Pigeonpea Roots is Associated with the Activation of Estrogen Receptorα-dependent Signaling Pathway in Human Prostate Cancer Cells.
Phytother Res.2013 Dec;27(12):1834-41.
55.Qin X, Sun ZQ, Zhang XW, Dai XJ, Mao SS, Zhang YM.
TLR4 signaling is involved in the protective effect of propofol in BV2 microglia against OGD/reoxygenation.
J Physiol Biochem. 2013 Dec;69(4):707-18.
56.Wu K, Xu J, Liu M, Song W, Yan J, Gao S, Zhao L, Zhang Y.
Induction of osteogenic differentiation of stem cells via a lyophilized microRNA reverse transfection formulationon a tissue culture plate.
Int J Nanomedicine. 2013;8:1595-607.
57.Li Q, Zhao XL, Sun J, Jiang SG, Gong XF.
Anti-proliferative and apoptosis-inducing activities of juglone in LS-174T cells.
Bangladesh Journal of Pharmacology.2013;8(1):65-72.
58.Zhang P, Wu Y, Dai Q, Fang B, Jiang L.
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Mol Cell Biochem. 2013 Jun;378(1-2):19-28.
59.Zhang E, Yan X, Zhang M, Chang X, Bai Z, He Y, Yuan Z.
Aggrecanases in the human synovial fluid at different stages of osteoarthritis.
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60.Chen M, Zhou M, Ye Z, Zhou Y, Tan WS.
Ectopic Osteogenesis of Macroscopic Tissue Constructs Assembled from Human Mesenchymal Stem Cell-Laden Microcarriers through In Vitro Perfusion Culture.
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61.Lin Z, Feng R, Li J, Meng Y, Yuan L, Fu Z, Guo J, Bringhurst FR, Yang D.
Nuclear translocation of CBP/p300-interacting protein CITED1 induced by parathyroid hormone requires serine phosphorylation at position 79 in its 63-84 domain.
Cell Signal. 2014 Nov;26(11):2436-45.
62.Ji CF, Ji YB.
Apoptosis of human gastric cancer SGC-7901 cells induced by podophyllotoxin.
Exp Ther Med. 2014 May;7(5):1317-22.  
63.Shi JX, Li JS, Hu R, Shi Y, Su X, Li Q, Zhang F.
CNOT7/hCAF1 is involved in ICAM-1 and IL-8 regulation by tristetraprolin.
Cell Signal. 2014 Nov;26(11):2390-6.
64.Xu Q, Li B, Yuan L, Dong Z, Zhang H, Wang H, Sun J, Ge S, Jin Y.
Combination of platelet-rich plasma within periodontal ligament stem cell sheets enhances cell differentiation and matrix production.
J Tissue Eng Regen Med.2014 Sep 4. doi: 10.1002/term.1953. 
65.Xie B, Wang J, Liu S, Wang J, Xue B, Li J, Wei R, Zhao Y, Liu Z.
Positive correlation between the efficiency of induced pluripotent stem cells and the development rate of nuclear transfer embryos when the same porcine embryonic fibroblast lines are used as donor cells.
Cell Reprogram. 2014 Jun;16(3):206-14.
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Tristetraprolin is involved in the glucocorticoid-mediated interleukin 8 repression.
Int Immunopharmacol. 2014 Oct;22(2):480-5.
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The effect of gold nanoparticles on the proliferation and differentiation of murine osteoblast: a study of MC3T3-E1 cells in vitro.
J Nanosci Nanotechnol. 2014 Jul;14(7):4851-7.
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Bone regeneration using photocrosslinked hydrogel incorporating rhBMP-2 loaded 2-N, 6-O-sulfated chitosan nanoparticles.
Biomaterials. 2014 Mar;35(9):2730-42.
69.Du J, Wu Y, Ai Z, Shi X, Chen L, Guo Z.
Mechanism of SB431542 in inhibiting mouse embryonic stem cell differentiation.
Cell Signal. 2014 Oct;26(10):2107-16.
70.Xie Q, Wang Z, Bi X, Zhou H, Wang Y, Gu P, Fan X.
Effects of miR-31 on the osteogenesis of human mesenchymal stem cells.
Biochem Biophys Res Commun. 2014 Mar 28;446(1):98-104.
71.Yan J, Zhang C, Zhao Y, Cao C, Wu K, Zhao L, Zhang Y.
Non-viral oligonucleotide antimiR-138 delivery to mesenchymal stem cell sheets and the effect on osteogenesis.
Biomaterials. 2014 Sep;35(27):7734-49.
72.Li P, Wang T, Mao Y, Zhang Y, Niu L, Liang R, Zhu L, Luo X.
Effect of ultimate pH on postmortem myofibrillar protein degradation and meat quality characteristics of Chinese Yellow crossbreed cattle.
ScientificWorldJournal. 2014;2014:174253.
73.Guo D, Li Q, Lv Q, Wei Q, Cao S, Gu J.
MiR-27a targets sFRP1 in hFOB cells to regulate proliferation, apoptosis and differentiation.
PLoS One. 2014 Mar 13;9(3):e91354.
74.Wang W, Liu Q, Zhang Y, Zhao L.
Involvement of ILK/ERK1/2 and ILK/p38 pathways in mediating the enhanced osteoblast differentiation by micro/nanotopography.
Acta Biomater. 2014 Aug;10(8):3705-15.
75.Yang M, Shuai Y, Zhang C, Chen Y, Zhu L, Mao C, OuYang H.
Biomimetic nucleation of hydroxyapatite crystals mediated by Antheraea pernyi silk sericin promotes osteogenic differentiation of human bone marrow derived mesenchymal stem cells.
Biomacromolecules. 2014 Apr 14;15(4):1185-93. 
76.Wang J, An Y, Li F, Li D, Jing D, Guo T, Luo E, Ma C.
The effects of pulsed electromagnetic field on the functions of osteoblasts on implant surfaces with different topographies.
Acta Biomater. 2014 Feb;10(2):975-85.
77.Chen Y, Zheng L, Liu J, Zhou Z, Cao X, Lv X, Chen F.
Shikonin inhibits prostate cancer cells metastasis by reducing matrix metalloproteinase-2/-9 expression via AKT/mTOR and ROS/ERK1/2 pathways.
Int Immunopharmacol. 2014 Aug;21(2):447-55.
78.Liu Y, Liu Y, Zhang R, Wang X, Huang F, Yan Z, Nie M, Huang J, Wang Y, Wang Y, Chen L, Yin L, He B, Deng Z.
All-trans retinoic acid modulates bone morphogenic protein 9-induced osteogenesis and adipogenesis of preadipocytes through BMP/Smad and Wnt/β-catenin signaling pathways.
Int J Biochem Cell Biol. 2014 Feb;47:47-56.
79.Xiao J, Gao H, Jin Y, Zhao Z, Guo J, Liu Z, Zhao Z.
The abnormal expressions of tristetraprolin and the VEGF family in uraemic rats with peritoneal dialysis.
Mol Cell Biochem. 2014 Jul;392(1-2):229-38.
80.Wang C, Lin K, Chang J, Sun J.
The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular endothelial growth factor secretion of endothelial cells by β-CaSiO3/β-Ca3(PO4)2 scaffolds.
J Biomed Mater Res A. 2014 Jul;102(7):2096-104.
81.Guo H, Wang Y, Song T, Xin T, Zheng Z, Zhong P, Zhang X.
Silencing of Survivin Using YM155 Inhibits Invasion and Suppresses Proliferation in Glioma Cells.
Cell Biochem Biophys.2015 Mar;71(2):587-93. 
82.Zhao S, Deng C, Wang Z, Teng L, Chen J.
Heparan sulfate 6-O-sulfotransferase 3 is involved in bone marrow mesenchymal stromal cell osteogenic differentiation.
Biochemistry (Mosc).2015 Mar;80(3):379-89.
83.Fu Y, Kadioglu O, Wiench B, Wei Z, Gao C, Luo M, Gu C, Zu Y, Efferth T.
Cell cycle arrest and induction of apoptosis by cajanin stilbene acid from Cajanus cajan in breast cancer cells.
Phytomedicine.2015 Apr 15;22(4):462-8.
84.Gao B, Huang Q, Jie Q, Wang L, Zhang HY, Liu J, Yang L, Luo ZJ.
Dose-response estrogen promotes osteogenic differentiation via GPR40 (FFAR1) in murine BMMSCs.
Biochimie.2015 Mar;110:36-44.
85.Wang Z, Zhang D, Hu Z, Cheng J, Zhuo C, Fang X, Xing Y.
MicroRNA-26a-modified adipose-derived stem cells incorporated with a porous hydroxyapatite scaffold improve the repair of bone defects.
Mol Med Rep.2015 Sep;12(3):3345-50.
86.Li Y, Jiao Y, Li X, Guo Z.
Improving the osteointegration of Ti6Al4V by zeolite MFI coating.
Biochem Biophys Res Commun.2015 May 1;460(2):151-6.
87.Daqian W, Chuandong W, Xinhua Q, Songtao A, Kerong D.
Chimaphilin inhibits proliferation and induces apoptosis in multidrug resistant osteosarcoma cell lines through insulin-like growth factor-I receptor (IGF-IR) signaling.
Chem Biol Interact. 2015 Jul 25;237:25-30.
88.Su X, Liao L, Shuai Y, Jing H, Liu S, Zhou H, Liu Y, Jin Y.
MiR-26a functions oppositely in osteogenic differentiation of BMSCs and ADSCs depending on distinct activation and roles of Wnt and BMP signaling pathway. 
Cell Death Dis.2015 Aug 6;6:e1851.
89.Zhao S, Hao J, Xue Y, Liang C.
Intracellular localization of rice stripe virus RNA-dependent RNA polymerase and its interaction with nucleocapsid protein.
Virus Genes. 2015 Dec;51(3):423-9.
90.Shen WW, Chen WG, Liu FZ, Hu X, Wang HK, Zhang Y, Chu TW.
Breast cancer cells promote osteoblastic differentiation via Sema 3A signaling pathway in vitro.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1584-93.
91.Dai XJ, Li N, Yu L, Chen ZY, Hua R, Qin X, Zhang YM.
Activation of BV2 microglia by lipopolysaccharide triggers an inflammatory reaction in PC12 cell apoptosis through a toll-like receptor 4-dependent pathway.
Cell Stress Chaperones. 2015 Mar;20(2):321-31.
92.Zhou C, Cai X, Fu Y, Wei X, Fu N, Xie J, Lin Y.
Tetraploid complementation proves pluripotency of induced pluripotent stem cells derived from adipose tissue.
Cell Prolif. 2015 Feb;48(1):39-46.
93.Wang J, Wei R, Bou G, Liu Z.
Tbx3 and Nr5α2 improve the viability of porcine induced pluripotent stem cells after dissociation into single cells by inhibiting RHO-ROCK-MLC signaling.
Biochem Biophys Res Commun. 2015 Jan 16;456(3):743-9.
94.Yang J, Wu M, Zhang X, Guo M, Huang Z.
Expression of Agrobacterium homolog genes encoding T-complex recruiting protein under virulence induction conditions.
Front Microbiol. 2015 Dec 2;6:1379.
95.Ju Y, Ge J, Ren X, Zhu X, Xue Z, Feng Y, Zhao S.
Ca v 1.2 of L-type Calcium Channel Is a Key Factor for the Differentiation of Dental Pulp Stem Cells.
J Endod. 2015 Jul;41(7):1048-55.
96.Yin Z, Chen X, Song HX, Hu JJ, Tang QM, Zhu T, Shen WL, Chen JL, Liu H, Heng BC, Ouyang HW.
Electrospun scaffolds for multiple tissues regeneration in vivo through topography dependent induction of lineage specific differentiation.
Biomaterials.2015 Mar;44:173-85.
97.Li J, Hu C, Han L, Liu L, Jing W, Tang W, Tian W, Long J. 
MiR-154-5p regulates osteogenic differentiation of adipose-derived mesenchymal stem cells under tensile stress through the Wnt/PCP pathway by targeting Wnt11. 
Bone.2015 Sep;78:130-41.
98.Lu Q, Zuo WZ, Ji XJ, Zhou YX, Liu YQ, Yao XQ, Zhou XY, Liu YW, Zhang F, Yin XX.
Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy.
Phytomedicine.2015 Nov 15;22(12):1071-8.
99.Wang P, Peng X, Wei ZF, Wei FY, Wang W, Ma WD, Yao LP, Fu YJ, Zu YG.
Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzyme expression via PI3K/AKT and ERK1/2 pathway.
Biochim Biophys Acta.2015 Sep;1850(9):1751-61.
100.Chen X, Gu S, Chen BF, Shen WL, Yin Z, Xu GW, Hu JJ, Zhu T, Li G, Wan C, Ouyang HW, Lee TL, Chan WY.
Nanoparticle delivery of stable miR-199a-5p agomir improves the osteogenesis of human mesenchymal stem cells via the HIF1a pathway.
Biomaterials. 2015 Jun;53:239-50.
101.Zhang P, Dai Q, Ouyang N, Yang X, Wang J, Zhou S, He N, Fang B, Jiang L.
Mechanical Strain Promotes Osteogenesis of BMSCs from Ovariectomized Rats via the ERK1/2 but not p38 or JNK-MAPK Signaling Pathways.
Curr Mol Med. 2015;15(8):780-9.
102.Xie Q, Wang Z, Huang Y, Bi X, Zhou H, Lin M, Yu Z, Wang Y, Ni N, Sun J, Wu S, You Z, Guo C, Sun H, Wang Y, Gu P, Fan X. 
Characterization of human ethmoid sinus mucosa derived mesenchymal stem cells (hESMSCs) and the application of hESMSCs cell sheets in bone regeneration. 
Biomaterials.2015 Oct;66:67-82.
103.Meng S, Zhang X, Xu M, Heng BC, Dai X, Mo X, Wei J, Wei Y, Deng X.
Effects of deer age on the physicochemical properties of deproteinized antler cancellous bone: an approach to optimize osteoconductivity of bone graft.
Biomed Mater.2015 Jun 4;10(3):035006.
104.Fu Y, Kadioglu O, Wiench B, Wei Z, Wang W, Luo M, Yang X, Gu C, Zu Y, Efferth T.
Activity of the antiestrogenic cajanin stilbene acid towards breast cancer. 
J Nutr Biochem.2015 Nov;26(11):1273-82.
105.Pan S, Chen W, Liu X, Xiao J, Wang Y, Liu J, Du Y, Wang Y, Zhang Y.
Application of a Novel Population of Multipotent Stem Cells Derived from Skin Fibroblasts as Donor Cells in Bovine SCNT.
PLoS One.2015 Jan 20;10(1):e0114423.
106.Zhang C, Yuan H, Liu H, Chen X, Lu P, Zhu T, Yang L, Yin Z, Heng BC, Zhang Y, Ouyang H.
Well-aligned chitosan-based ultrafine fibers committed teno-lineage differentiation of human induced pluripotent stem cells for Achilles tendon regeneration.
Biomaterials.2015;53:716-30.
107.Song R, Li G, Li S.
Aspidin PB, a novel natural anti-fibrotic compound, inhibited fibrogenesis in TGF-β1-stimulated keloid fibroblasts via PI-3K/Akt and Smad signaling pathways.
Chem Biol Interact.2015 Aug 5;238:66-73.
108.Liu X, Shi S, Feng Q, Bachhuka A, He W, Huang Q, Zhang R, Yang X, Vasilev K. 
Surface Chemical Gradient Affects the Differentiation of Human Adipose-Derived Stem Cells via ERK1/2 Signaling Pathway. 
ACS Appl Mater Interfaces. 2015 Aug 26;7(33):18473-82。
109.Lu Q, Ji XJ, Zhou YX, Yao XQ, Liu YQ, Zhang F, Yin XX.
Role of Quercetin inhibits the mTORC1/p70S6K signaling-mediated renal tubular epithelial–mesenchymal transition and renal fibrosis in diabetic nephropathy.
Pharmacol Res.2015 Sep;99:237-47.
110.Sang H, Liu L, Wang L, Qiu Z, Li M, Yu L, Zhang H, Shi R, Yu S, Guo R, Ye R, Liu X, Zhang R.
Opposite Roles of Bradykinin B1 and B2 Receptors during Cerebral Ischemia/Reperfusion Injury in Experimental Diabetic Rats. 
Eur J Neurosci. 2016 Jan;43(1):53-65. 
111.Liu S, Jin D, Wu JQ, Xu ZY, Fu S, Mei G, Zou ZL, Ma SH.
Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro.
Neuropeptides. 2016 Jan;43(1):53-65. 
112.Xie Q, Wang Z, Zhou H, Yu Z, Huang Y, Sun H, Bi X, Wang Y, Shi W, Gu P, Fan X.
The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration.
Biomaterials.2016 Jan;75:279-94.
113.Xie B, Zhang H, Wei R, Li Q, Weng X, Kong Q, Liu Z.
Histone H3 lysine 27 trimethylation acts as an epigenetic barrier in porcine nuclear reprogramming.
Reproduction.2016 Jan;151(1):9-16.
114.Jiang Y, Du M, Wu M, Zhu Y, Zhao X, Cao X, Li X, Long P, Li W, Hu B.
Phosphatidic Acid Improves Reprogramming to Pluripotency by Reducing Apoptosis.
Stem Cells Dev.2016 Jan 1;25(1):43-54.
115.Tang W, Lin D, Yu Y, Niu H, Guo H, Yuan Y, Liu C. 
Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration of critical size bone defect.
Acta Biomater.2016 Mar;32: 309-23.
116.Wen L, Wang Y, Wen N, Yuan G, Wen M, Zhang L, Liu Q, Liang Y, Cai C, Chen X, Ding Y.
Role of Endothelial Progenitor Cells in Maintaining Stemness and Enhancing Differentiation of Mesenchymal Stem Cells by Indirect Cell–Cell Interaction.
Stem Cells Dev.2016 Jan 15;25(2):123-38.
117.Zhang G, Zhao BX, Hua R, Kang J, Shao BM, Carbonaro TM, Zhang YM.
Hippocampal microglial activation and glucocorticoid receptor down-regulation precipitate visceral hypersensitivity induced by colorectal distension in rats. 
Neuropharmacology. 2016 Mar;102:295-303. 
118.Huang C, Geng J, Wei X, Zhang R, Jiang S .
MiR-144-3p regulates osteogenic differentiation and proliferation of murine mesenchymal stem cells byspecifically targeting Smad4.
FEBS Lett. 2016 Mar;590(6):795-807.
119.Liu X, Liu Y, Hao J, Zhao X, Lang Y, Fan F, Cai C, Li G, Zhang L , Yu G.
In Vivo Anti-Cancer Mechanism of Low-Molecular-Weight Fucosylated Chondroitin Sulfate (LFCS) fromSea Cucumber Cucumaria frondosa.
Molecules. 2016 May 12;21(5). pii: E625.
120.Liu X, Yu B, Huang Q, Liu R, Feng Q, Cai Q, Mi S.
In vitro BMP-2 peptide release from thiolated chitosan based hydrogel.
Int J Biol Macromol. 2016 Dec;93(Pt A):314-321.
121.Zhang G, Zhao BX, Hua R, Kang J, Shao BM, Carbonaro TM, Zhang YM.
Hippocampal microglial activation and glucocorticoid receptor down-regulation precipitate visceralhypersensitivity induced by colorectal distension in rats.
Neuropharmacology. 2016 Mar;102:295-303.
122.Gao F, Wang Y, Li S, Wang Z, Liu C, Sun D.
Inhibition of p38 mitogen-activated protein kinases attenuates renal interstitial fibrosis in a murineunilateral ureteral occlusion model.
Life Sci. 2016 Dec 15;167:78-84.
123.Han QA, Yan C, Wang L, Li G, Xu Y, Xia X.
Urolithin A attenuates ox-LDL-induced endothelial dysfunction partly by modulating microRNA-27 and ERK/PPAR-γ pathway.
Mol Nutr Food Res. 2016 Sep;60(9):1933-43.
124.Wang P, Qiao Q, Li J, Wang W, Yao LP, Fu YJ.
Inhibitory effects of geraniin on LPS-induced inflammation via regulating NF-κB and Nrf2 pathways inRAW 264.7 cells.
Chem Biol Interact. 2016 Jun 25;253:134-42.
125.Jiang Y, Wang Y, Tang G.
Cyclic tensile strain promotes the osteogenic differentiation of a bone marrow stromal cell and vascularendothelial cell co-culture system.
Arch Biochem Biophys. 2016 Oct 1;607:37-43.
126.Wang X, Gao Y, Shi H, Liu N, Zhang W, Li H.
Influence of the intensity and loading time of direct current electric field on the directional migration ofrat bone marrow mesenchymal stem cells.
Front Med. 2016 Sep;10(3):286-96.
127.Qiao X, Nie Y, Ma Y, Chen Y, Cheng R, Yin W, Hu Y, Xu W, Xu L.
Irisin promotes osteoblast proliferation and differentiation via activating the MAP kinase signaling pathways.
Sci Rep. 2016 Jan 7;6:18732.
128.Li Y, Zheng Z, Cao Z, Zhuang L, Xu Y, Liu X, Xu Y, Gong Y.
Enhancing proliferation and osteogenic differentiation of HMSCs on casein/chitosan multilayer films.
Colloids Surf B Biointerfaces. 2016 May 1;141:397-407.
129.Liang Y, Wen L, Shang F, Wu J, Sui K, Ding Y.
Endothelial progenitors enhanced the osteogenic capacities of mesenchymal stem cells in vitro and in arat alveolar bone defect model.
Arch Oral Biol. 2016 Aug;68:123-30.
130.Zhang G, Yu L, Chen ZY, Zhu JS, Hua R, Qin X, Cao JL, Zhang YM.
Activation of corticotropin-releasing factor neurons and microglia in paraventricular nucleus precipitatesvisceral hypersensitivity induced by colorectal distension in rats.
Brain Behav Immun. 2016 Jul;55:93-104.
131.Liu S, Jin D, Wu JQ, Xu ZY, Fu S, Mei G, Zou ZL, Ma SH.
Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrowmesenchymal stem cells related to canonical Wnt signaling activating in vitro.
Neuropeptides. 2016 Apr;56:105-13.
132. Li S, Hu C, Li J, Liu L, Jing W, Tang W, Tian W, Long J.
Effect of miR-26a-5p on the Wnt/Ca(2+) Pathway and Osteogenic Differentiation of Mouse Adipose-Derived Mesenchymal Stem Cells.
Calcif Tissue Int. 2016 Aug;99(2):174-86.
133.Zhai JW, Gao C, Ma WD, Wang W, Yao LP, Xia XX, Luo M, Zu YG, Fu YJ.
Geraniin induces apoptosis of human breast cancer cells MCF-7 via ROS-mediated stimulation of p38MAPK.
Toxicol Mech Methods. 2016 Jun;26(5):311-8.
134.Huang Q, Elkhooly TA, Liu X, Zhang R, Yang X, Shen Z, Feng Q.
SaOS-2 cell response to macro-porous boron-incorporated TiO2 coating prepared by micro-arc oxidation on titanium.
Mater Sci Eng C Mater Biol Appl. 2016 Oct 1;67:195-204.
135.Lan JF, Wei S, Wang YQ, Dai YJ, Tu JG, Zhao LJ, Li XC, Qin QW, Chen N, Lin L.
PcToll3 was involved in anti-Vibrio response by regulating the expression of antimicrobial peptides inred swamp crayfish, Procambarus clarkii.
Fish Shellfish Immunol. 2016 Oct;57:17-24.
136.Hu JJ, Yin Z, Shen WL, Xie YB, Zhu T, Lu P, Cai YZ, Kong MJ, Heng BC, Zhou YT, Chen WS, Chen X, Ouyang HW.
Pharmacological Regulation of In Situ Tissue Stem Cells Differentiation for Soft Tissue CalcificationTreatment.
Stem Cells. 2016 Apr;34(4):1083-96.
137.Fu X, Yang H, Zhang H, Wang G, Liu K, Gu Q, Tao Y, Chen G, Jiang X, Li G, Gu Y, Shi Q.
Improved osteogenesis and upregulated immunogenicity in human placenta-derived mesenchymal stemcells primed with osteogenic induction medium.
Stem Cell Res Ther. 2016 Sep 20;7(1):138.
138.Zhang Y, Ma J, Li H, Lv J, Wei R, Cong Y, Liu Z.
bFGF signaling-mediated reprogramming of porcine primordial germ cells.
Cell Tissue Res. 2016 May;364(2):429-41.
139.Xu J, Wang Y, Li J, Zhang X, Geng Y, Huang Y, Dai K, Zhang X.
IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4+ T cells.
Cell Death Differ. 2016 Dec;23(12):1941-1951.
140.Tang W, Lin D, Yu Y, Niu H, Guo H, Yuan Y, Liu C.
Bioinspired trimodal macro/micro/nano-porous scaffolds loading rhBMP-2 for complete regeneration ofcritical size bone defect.
Acta Biomater. 2016 Mar 1;32:309-23.
141.Lin J, Chen F, Sun MJ, Zhu J, Li YW, Pan LZ, Zhang J, Tan JH.
The relationship between apoptosis, chromatin configuration, histone modification and competence ofoocytes: A study using the mouse ovary-holding stress model.
Sci Rep. 2016 Jun 20;6:28347.
142.Wu RX, Yu Y, Yin Y, Zhang XY, Gao LN, Chen FM.
Platelet lysate supports the in vitro expansion of human periodontal ligament stem cells forcytotherapeutic use.
J Tissue Eng Regen Med. 2016 Feb 2. doi: 10.1002/term.2124. [Epub ahead of print]
143.Wang Z, Lin M, Xie Q, Sun H, Huang Y, Zhang D, Yu Z, Bi X, Chen J, Wang J, Shi W, Gu P, Fan X.
Electrospun silk fibroin/poly(lactide-co-ε-caprolactone) nanofibrous scaffolds for bone regeneration.
Int J Nanomedicine. 2016 Apr 11;11:1483-500.
144.Li J, Zhao PP, Hao T, Wang D, Wang Y, Zhu YZ, Wu YQ, Zhou CH.
Urotensin II inhibitor eases neuropathic pain by suppressing the JNK/NF-κB pathway.
J Endocrinol. 2017 Feb;232(2):165-174.
145.Li X, Zhang X, Xie W, Zhou C, Li Y, Zhang X.
Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused bymicrocystin-LR.
Toxicol In Vitro. 2017 Jan 3;40:115-123.
146.Chen W, Shen X, Hu Y, Xu K, Ran Q, Yu Y, Dai L, Yuan Z, Huang L, Shen T, Cai K.
Surface functionalization of titanium implants with chitosan-catechol conjugate for suppression of ROS-induced cells damage and improvement of osteogenesis.
Biomaterials. 2017 Jan;114:82-96.