Rhodamine 123也称2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester，中文名为罗丹明123，分子式为C₂₁H₁₇ClN₂O₃，分子量为380.82，CAS Number 62669-70-9，纯度>95%。
      Rhodamine 123是一种可以通透细胞膜的选择性染色活细胞线粒体的阳离子黄绿色荧光染料，广泛用作检测线粒体膜电位(mitochondrial membrane potential)的荧光探针，也常用于细胞凋亡检测。在正常细胞中，Rhodamine 123能够依赖线粒体跨膜电位(mitochondrial transmembrane potential, ΔΨm)选择性进入线粒体基质，荧光强度减弱或消失(quench)；在凋亡发生时，线粒体膜完整性被破坏，线粒体膜通透性转运孔开放，从而引起线粒体跨膜电位的崩溃，Rhodamine 123从线粒体基质中释放出来，黄绿色荧光增强(dequench)。该荧光可用荧光显微镜、流式细胞仪或其它荧光检测设备检测，通过荧光信号的强弱来确定线粒体膜电位的变化和凋亡的发生。
      Rhodamine 123可以快速通过细胞膜，仅需几分钟就可以被具有活性的线粒体所俘获(sequestered by active
mitochondria)，并且对细胞没有任何毒性。
      Rhodamine 123的激发光谱和发射光谱参考下图，最大激发波长为507nm，最大发射波长为529nm。在荧光显微镜下观
察，呈现黄绿色荧光。

      Rhodamine 123为红棕色粉末，溶于DMSO，也溶于乙醇，在乙醇中的溶解度可达20mg/ml。
包装清单：

保存条件：
      室温避光保存，两年有效。溶于DMSO配制成母液后-20℃保存。
注意事项：
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
      为了您的安全和健康，请穿实验服并戴一次性手套操作

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