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 Annexin V-FITC细胞凋亡检测试剂盒

  产品编号C1063
  产品包装: 50次
  产品价格: 1068.00元
产品编号 产品名称 产品包装 产品价格
C1063 Annexin V-FITC细胞凋亡检测试剂盒 50次 1068.00元

      Annexin V-FITC细胞凋亡检测试剂盒(Annexin V-FITC Apoptosis Detection Kit)是用FITC标记的重组人Annexin V来检测细胞凋亡时出现在细胞膜表面的磷脂酰丝氨酸的一种细胞凋亡检测试剂盒。可以使用流式细胞仪、荧光显微镜或其它荧光检测设备进行检测。
      Annexin是一类广泛分布于真核细胞细胞浆内钙离子依赖的磷脂结合蛋白,参与细胞内的信号转导。但仅Annexin V被报道可以调控一些PKC的活性。
      Annexin V选择性结合磷脂酰丝氨酸(phosphatidylserine,简称PS)。磷脂酰丝氨酸主要分布在细胞膜内侧,即与细胞浆相邻的一侧。在细胞发生凋亡的早期,不同类型的细胞都会把磷脂酰丝氨酸外翻到细胞表面,即细胞膜外侧。磷脂酰丝氨酸暴露到细胞表面后会促进凝血和炎症反应。而Annexin V和外翻到细胞表面的磷脂酰丝氨酸结合后可以阻断磷脂酰丝氨酸的促凝血和促炎症反应活性。
      用带有绿色荧光的荧光探针FITC标记的Annexin V,即Annexin V-FITC,就可以用流式细胞仪或荧光显微镜非常简单而直接地检测到磷脂酰丝氨酸的外翻这一细胞凋亡的重要特征。
      本试剂盒还提供了碘化丙啶(Propidium Iodide, PI)染色液,碘化丙啶可以染色坏死细胞或凋亡晚期丧失细胞膜完整性的细胞,呈现红色荧光。对于坏死细胞,由于细胞膜的完整性已经丧失,Annexin V-FITC可以进入到细胞浆内,与位于细胞膜内侧的磷脂酰丝氨酸结合,从而也使坏死细胞呈现绿色荧光。
      本试剂盒的流式检测效果参考图1和图2,荧光显微镜检测效果参考图3。

      图1.细胞用本试剂盒染色后流式细胞仪检测细胞凋亡的效果图。Jurkat细胞未经处理(A)或用10μM喜树碱(Camptothecin)作用4小时(B)后,用本试剂盒染色,然后用流式细胞仪进行细胞的散射和荧光检测。从图中可以看出,经过凋亡诱导剂喜树碱处理后的细胞,其Annexin V-FITC染色阳性并且PI染色阴性的细胞,即凋亡细胞,明显增加(B图的右下象限),Annexin V-FITC和PI染色双阳性的细胞,即坏死细胞,也有所增加(B图的右上象限)。图中Annexin V-FITC染色阴性PI染色阳性(Annexin V-/PI+)所在象限(A和B图的左上象限)出现的细胞点是许可范围内的检测误差。实测数据可能会因细胞类型、细胞凋亡情况、检测仪器等的不同而存在差异,图中数据仅供参考。

      图2.细胞用本试剂盒染色后流式细胞仪检测效果验证图。Jurkat细胞用10μM喜树碱(Camptothecin)作用4小时后,未经染色(A)、仅用本试剂盒中的PI进行染色(B)、仅用本试剂盒中的Annexin V-FITC进行染色(C)、用本试剂盒中的Annexin V-FITC和PI进行双染(D)。从图中可以看出,未经染色的是双阴性细胞(A),仅PI染色后出现了预期的PI染色阳性的细胞,仅Annexin V-FITC染色出现了预期的Annexin V-FITC染色阳性的细胞,Annexin V-FITC和PI双染出现了预期的仅Annexin V-FITC染色阳性的凋亡细胞和双阳性的坏死细胞。出现的非预期的细胞点完全在许可的误差范围内。实测数据可能会因细胞类型、细胞凋亡情况、检测仪器等的不同而存在差异,图中数据仅供参考。

      一个包装的本试剂盒共可以检测50个细胞样品。
包装清单:

产品编号 产品名称 包装
C1063-1 Annexin V-FITC 250μl
C1063-2 Annexin V-FITC结合液 26ml
C1063-3 碘化丙啶染色液 550μl
说明书 1份

保存条件:
      4℃保存,半年有效。Annexin V-FITC和碘化丙啶染色液需要避光保存。为了长期保存,可以把碘化丙啶染色液适当分装后
-20℃保存,Annexin V-FITC结合液可以直接-20℃保存。
注意事项:
      如果有细菌或真菌污染,会严重影响检测效果。
      染色后宜尽快检测,时间过长可能会导致凋亡或坏死细胞的数量增加。
      如果细胞收集过程中使用了胰酶,需注意设法去除残留的胰酶。残留的胰酶会消化并降解Annexin V-FITC,导致染色失败。
      荧光物质均易发生淬灭,在进行荧光观察时,尽量缩短观察时间,同时在操作和存放过程中也尽量注意避光保存。
      用于流式细胞仪检测时,如果发现Annexin V-FITC单独染色时出现了过多的PI假阳性细胞,并且通过调整相关设置和参数也无法改善,可以用PBS将Annexin V-FITC稀释3-10倍后再进行检测,这样通常可以有效减少假阳性的坏死细胞。
      需自备PBS。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 对于悬浮细胞:
a. 在进行完细胞凋亡刺激后,1000g离心5分钟,弃上清,收集细胞,用PBS轻轻重悬细胞并计数。注意:PBS重悬不能省略,PBS重悬的过程同时也起到了洗涤细胞的作用,可以保证后续Annexin V-FITC的结合。
b. 取5-10万重悬的细胞,1000g离心5分钟,弃上清,加入195μl Annexin V-FITC结合液轻轻重悬细胞。
c. 加入5μl Annexin V-FITC,轻轻混匀。
d. 加入10μl碘化丙啶染色液,轻轻混匀。
e. 室温(20-25℃)避光孵育10-20分钟,随后置于冰浴中。可以使用铝箔进行避光。孵育过程中可以重悬细胞2-3次以改善染色效果。
f. 如果用于流式细胞仪检测,可立即上机检测,Annexin V-FITC为绿色荧光,碘化丙啶(PI)为红色荧光,流式检测细胞凋亡的效
果及其验证请参考图1和图2。初次进行流式细胞仪检测时,建议选择一组适当的细胞参考图2设置未染色、PI单染和Annexin V-FITC单染这3个对照。如果用于荧光显微镜检测,1000g离心5分钟,收集细胞,用50-100μl Annexin V-FITC结合液轻轻重悬细胞,涂片后,荧光显微镜下观察。注意:细胞在染色后须尽快完成检测,通常宜在1小时之内完成检测。用于流式细胞仪检测时,如果发现Annexin V-FITC单独染色时出现了过多的PI假阳性细胞,并且通过调整相关设置和参数也无法改善,可以用PBS将Annexin V-FITC稀释3-10倍后再进行检测。
2. 对于贴壁细胞的消化后检测:
a. 把细胞培养液吸出至一合适离心管内,PBS洗涤贴壁细胞一次,加入适量胰酶细胞消化液(可含有EDTA)消化细胞。室温孵育至轻轻吹打可以使贴壁细胞吹打下来时,吸除胰酶细胞消化液。需避免胰酶的过度消化。注意:对于贴壁细胞,胰酶消化步骤很关键。胰酶消化时间如果过短,细胞需要用力吹打才能脱落,容易造成细胞膜的损伤,从而导致细胞坏死的假阳性;消化时间如果过长,同样易造成细胞膜损伤而出现细胞坏死的假阳性,甚至会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合从而干扰对于细胞凋亡的检测。同时,胰酶细胞消化液中应尽量不含EDTA,因为EDTA可能会影响Annexin V与磷脂酰丝氨酸的结合。
b. 加入步骤2a中收集的细胞培养液,把细胞轻轻吹打下来,转移到离心管内,1000g离心5分钟,弃上清,收集细胞,用PBS轻轻重悬细胞并计数。注意:加入步骤2a中的细胞培养液非常重要,一方面可以收集已经悬浮的发生凋亡或坏死的细胞,另一方面细胞培养液中的血清可以有效抑制或中和残留的胰酶。残留的胰酶会消化并降解后续加入的Annexin V-FITC,导致染色失败。
c. 取5-10万重悬的细胞,1000g离心5分钟,弃上清,加入195μl Annexin V-FITC结合液轻轻重悬细胞。
d. 加入5μl Annexin V-FITC,轻轻混匀。
e. 加入10μl碘化丙啶染色液,轻轻混匀。
f. 室温(20-25℃)避光孵育10-20分钟,随后置于冰浴中。可以使用铝箔进行避光。孵育过程中可以重悬细胞2-3次以改善染色效果。
g. 如果用于流式细胞仪检测,可立即上机检测,Annexin V-FITC为绿色荧光,碘化丙啶(PI)为红色荧光,流式检测的效果及其验
证请参考图1和图2。如果用于荧光显微镜检测,1000g离心5分钟,收集细胞,用50-100μl Annexin V-FITC结合液轻轻重悬细胞,涂片后,荧光显微镜下观察。注意:细胞在染色后须尽快完成检测,通常宜在1小时之内完成检测。用于流式细胞仪检测时,如果发现Annexin V-FITC单独染色时出现了过多的PI假阳性细胞,并且通过调整相关设置和参数也无法改善,可以用PBS
将Annexin V-FITC稀释3-10倍后再进行检测。
3. 对于贴壁细胞的原位荧光检测:
注:本方法的优点是可以原位观察细胞凋亡,缺点是部分凋亡由于不贴壁而检测不到。
a. (选做)如果条件许可,把细胞培养于24孔板、48孔板或96孔板内。在凋亡诱导结束后,用可以对多孔板进行离心的离心机1000g离心5分钟。
b. 吸除细胞培养液,加入PBS洗涤一次。如果条件许可,在吸除PBS前1000g离心5分钟。
c. 加入195μl Annexin V-FITC结合液。
d. 加入5μl Annexin V-FITC,轻轻混匀。
e. 加入10μl碘化丙啶染色液,轻轻混匀。
f. 室温(20-25℃)避光孵育10-20分钟,随后置于冰浴中。可以使用铝箔进行避光。
g. 随即在荧光显微镜下观察,Annexin V-FITC为绿色荧光,碘化丙啶(PI)为红色荧光。注意:细胞在染色后须尽快完成检测,通常宜在1小时之内完成检测。

图3. Annexin V-FITC和碘化丙啶(PI)染色效果图。图中绿色荧光为Annexin V-FITC染色阳性细胞,红色荧光为碘化丙啶染色阳性细胞。仅被绿色荧光染色的为凋亡细胞,被绿色和红色荧光双染的是坏死细胞,未被荧光染色的为正常细胞。

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15.Zhang C, Tan C, Zu X, Zhai X, Liu F, Chu B, Ma X, Chen Y, Gong P, Jiang Y.
Exploration of (S)-3-aminopyrrolidine as a potentially interesting scaffold for discovery of novelAbl and PI3K dual inhibitors.
Eur J Med Chem. 2011 Apr;46(4):1404-14.
16.Ai RT, Wu SY, Wen XY, Xu W, Lv L, Rao JJ, Wu SG.
1,3,4-tri-O-galloyl-6-O-caffeoyl-β-D-glucopyranose, a new anti-proliferative ellagitannin,regulatesthe expression of microRNAs in HepG(2) cancer cells.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Oct;31(10):1641-8.
17.Zhang R, Shi H, Chen Z, Wu Q, Ren F, Huang H.
Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovariancarcinoma OVCAR-3 cells.
J Exp Clin Cancer Res. 2011 Sep 16;30:83.
18.Li Y, Tan C, Gao C, Zhang C, Luan X, Chen X, Liu H, Chen Y, Jiang Y.
Discovery of benzimidazole derivatives as novel multi-target EGFR, VEGFR-2 and PDGFR kinaseinhibitors.
Bioorg Med Chem. 2011 Aug 1;19(15):4529-35.
19.Wang Z, Yang X, Yang S, Ren G, Ferreri M, Su Y, Chen L, Han B.
Sodium fluoride suppress proliferation and induce apoptosis through decreased insulin-likegrowth factor-I expression and oxidative stress in primary culturedmouse osteoblasts.
Arch Toxicol. 2011 Nov;85(11):1407-17. 
20.Cheng H, Qiu L, Zhang H, Cheng M, Li W, Zhao X, Liu K, Lei L, Ma J.
Arsenic trioxide promotes senescence and regulates the balance of adipogenic and osteogenicdifferentiation in human mesenchymal stem cells.
Acta Biochim Biophys Sin (Shanghai). 2011 Mar;43(3):204-9.
21.Zhao L, Hao J, Hu J, Wang Q, Lü Z, Wang L, Yu Q, Wang Y, Li G.
Expression of proliferin-related protein in testis and the biological significance in testosteroneproduction.
Mol Cell Endocrinol. 2011 Aug 22;343(1-2):25-31.
22.Xin H, Jiang X, Gu J, Sha X, Chen L, Law K, Chen Y, Wang X, Jiang Y, Fang X.
Angiopep-conjugated poly(ethylene glycol)-co-poly(ε-caprolactone) nanoparticles as dual-targetingdrug delivery system for brain glioma.
Biomaterials. 2011 Jun;32(18):4293-305.
23.Wang X, Xia X, Xu C, Xu J, Wang P, Xiang J, Bai D, Leung AW.
Ultrasound-induced cell death of nasopharyngeal carcinoma cells in the presence of curcumin.
Integr Cancer Ther. 2011 Mar;10(1):70-6.
24.Ma HM, Quan FS, Chen DM, Zheng YL, Zhang BW, Wang YS.
Protective function of alpha-lipoic acid on sperm motility and mitochondrial function during goat sperm-mediated gene transfer.
Small Ruminant Research.2011 Aug;99(2):191-8.
25.Tong JS, Zhang QH, Huang X, Fu XQ, Qi ST, Wang YP, Hou Y, Sheng J, Sun QY.
Icaritin causes sustained ERK1/2 activation and induces apoptosis in human endometrial cancercells.
PLoS One. 2011 Mar 8;6(3):e16781.
26.Yu H, Zhang T, Cai L, Qu Y, Hu S, Dong G, Guan R, Xu X, Xing L.
Chamaejasmine induces apoptosis in human lung adenocarcinoma A549 cells through a Ros-mediated mitochondrial pathway.
Molecules. 2011 Sep 27;16(10):8165-80.
27.Rasul A, Khan M, Yu B, Ma T, Yang H.
Xanthoxyletin, a coumarin induces S phase arrest and apoptosis in human gastric adenocarcinomaSGC-7901 cells.
Asian Pac J Cancer Prev. 2011;12(5):1219-23.
28.Yang YF, Chen Z, Hu SL, Hu J, Li B, Li JT, Wei LJ, Qian ZM, Lin JK, Feng H, Zhu G.
Interleukin-1 receptor associated kinases-1/4 inhibition protects against acute hypoxia/ischemia-induced neuronal injury in vivo and in vitro.
Neuroscience. 2011 Nov 24;196:25-34.
29.Chen LP, Zhao F, Wang Y, Zhao LL, Li QP, Liu HW.
Antitumor effect of resorcinol derivatives from the roots of Ardisia brevicaulis by inducingapoptosis.
J Asian Nat Prod Res. 2011 Aug;13(8):734-43.
30.Luo W, Xiong W, Zhou J, Fang Z, Chen W, Fan Y, Li F.
Laminar shear stress delivers cell cycle arrest and anti-apoptosis to mesenchymal stem cells.
Acta Biochim Biophys Sin (Shanghai). 2011 Mar;43(3):210-6.
31.Wang CM, Sheng GY, Lu J, Xie L, Bai ST, Xu XJ, Liu YF.
Effect of small interfering RNA targeting wild-type FLT3 in acute myeloid leukaemia cells in vitroand in vivo.
J Int Med Res. 2011;39(5):1661-74.
32.Zhong MR, Zhao YH, Zhang K, Yang LF, Zheng YC, He CY.
Inhibitory Effects of Natural Compound Alantolactone on Human Non-small Cell Lung Cancer A549 Cells.
CHEM. RES. CHINESE UNIVERSITIES. 2011;27(2):241-4.
33.Han JB, Tao ZZ, Chen SM, Kong YG, Xiao BK.
Adenovirus-mediated transfer of tris-shRNAs induced apoptosis of nasopharyngeal carcinoma cellin vitro and in vivo.
Cancer Lett. 2011 Oct 28;309(2):162-9.
34. Sun FL, Zhang L, Zhang RY, Li L.
Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells againstMPP+-induced cytotoxicity.
Eur J Pharmacol. 2011 Jun 25;660(2-3):283-90.
35.Zhang L, Zhang S.
ZM447439, the Aurora kinase B inhibitor, suppresses the growth of cervical cancer SiHa cells andenhances the chemosensitivity to cisplatin.
J Obstet Gynaecol Res. 2011 Jun;37(6):591-600.
36.Zhang Z, Song T, Zhang T, Gao J, Wu G, An L, Du G.
A novel BH3 mimetic S1 potently induces Bax/Bak-dependent apoptosis by targeting both Bcl-2and Mcl-1.
Int J Cancer. 2011 Apr 1;128(7):1724-35.
37.Xiang J, Xia X, Jiang Y, Leung AW, Wang X, Xu J, Wang P, Yu H, Bai D, Xu C.
Apoptosis of ovarian cancer cells induced by methylene blue-mediated sonodynamic action.
Ultrasonics. 2011 Apr;51(3):390-5.
38.Yu M, Dai J, Huang W, Jiao Y, Liu L, Wu M, Tan D.
hMTERF4 knockdown in HeLa cells results in sub-G1 cell accumulation and cell death.
Acta Biochim Biophys Sin (Shanghai). 2011 May;43(5):372-9.
39.Zheng F, Li H, Liang K, Du Y, Guo D, Huang S.
Imatinib has the potential to exert its antileukemia effects by down-regulating hERG1 K(+)channels in chronic myelogenous leukemia.
Med Oncol. 2012 Sep;29(3):2127-35.
40.Zou X, Feng Z, Li Y, Wang Y, Wertz K, Weber P, Fu Y, Liu J.
Stimulation of GSH synthesis to prevent oxidative stress-induced apoptosis by hydroxytyrosol inhuman retinal pigment epithelial cells: activation of Nrf2 and JNK-p62/SQSTM1 pathways.
J Nutr Biochem. 2012 Aug;23(8):994-1006.
41.Zhou BR, Yin HB, Xu Y, Wu D, Zhang ZH, Yin ZQ, Permatasari F, Luo D.
Baicalin protects human skin fibroblasts from ultraviolet A radiation-induced oxidative damage and apoptosis.
Free Radic Res. 2012 Dec;46(12):1458-71.
42.Huang XB,Wang F,Chen WQ,Chen YJ,Wang NQ, Maltzan K.
Possible link between the cognitive dysfunction associated with diabetes mellitus and the neurotoxicity of methylglyoxal.
Brain Research.2012 Aug;1469(1):82-91.
43.Tu ZZ,Li H,Ma YX,Tang B,Tian JM,Akers W,Achilefu S and Gu YQ.
The enhanced antiproliferative response to combined treatment of trichostatin A with raloxifene in MCF-7 breast cancer cells and its relevance to estrogen receptor β expression.
Molecular and Cellular Biochemistry.2012;366:111-22.
44.SU JQ,CHI BR,LI X,LIU L,LIU LM,QI YX,WANG ZY,JIN NY.
Inhibition of Dual Specific Oncolytic Adenovirus on Esophageal Cancer via Activation of Caspases by a Mitochondrial-dependent Pathway.
高等学校化学研究:英文版 CAS CSCD.2012;28(3):465-71.
45.Wang L, Xu Y, Fu L, Li Y, Lou L.
(5R)-5-hydroxytriptolide (LLDT-8), a novel immunosuppressant in clinical trials, exhibits potentantitumor activity via transcription inhibition.
Cancer Lett. 2012 Nov 1;324(1):75-82.
46.Li Y, Shi L, Han C, Wang Y, Yang J, Cao C, Jiao S.
Effects of ARHI on cell cycle progression and apoptosis levels of breast cancer cells.
Tumour Biol. 2012 Oct;33(5):1403-10.
47.Zhang Y, Lv Z, Zhong H, Geng D, Zhang M, Zhang T, Li Y, Li K.
Convenient synthesis of novel geiparvarin analogs with potential anti-cancer activity via clickchemistry.
Eur J Med Chem. 2012 Jul;53C:356-63.
48.Dai M, Xia XB, Xiong SQ.
BDNF regulates GLAST and glutamine synthetase in mouse retinal Müller cells.
J Cell Physiol. 2012 Feb;227(2):596-603.
49.Jiang C, Yi XP, Shen H, Li YX.
Targeting X-linked inhibitor of apoptosis protein inhibits pancreatic cancer cell growth through p-Akt depletion.
World J Gastroenterol. 2012 Jun 21;18(23):2956-65.
50.Huang HY, Cai JF, Liu QC, Hu GH, Xia B, Mao JY, Wu DS, Liu JJ, Zhuang ZX.
Role of poly(ADP-ribose) glycohydrolase in the regulation of cell fate in response tobenzo(a)pyrene.
Exp Cell Res. 2012 Mar 10;318(5):682-90.
51.Xu Y, Feng Y, Li H, Gao Z.
Ferric citrate CYP2E1-independently promotes alcohol-induced apoptosis in HepG2 cells viaoxidative/nitrative stress which is attenuated by pretreatment with baicalin.
Food Chem Toxicol. 2012 Sep;50(9):3264-72.
52.Jiang Y,Xia XS,Leung AW,Xiang JY,Xu CS.
Apoptosis of breast cancer cells induced by hypocrellin B under light-emitting diode irradiation.
Photodiagnosis and Photodynamic Therapy.2012 Dec;9(4):337-43.
53.Liu X, Zhao M, Lu J, Ma J, Wei J, Wei S.
Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities.
Int J Nanomedicine. 2012;7:1239-50.
54.Gu S, He J.
Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells.
Molecules. 2012 Jan 9;17(1):598-612.
55.Liu Y, Xiao Z, Yang D, Ren L, Liu G, Yang L.
Effects of the cyclin-dependent kinase 10 (CDK10) on the tamoxifen sensitivity of keloid samples.
Molecules. 2012 Feb 1;17(2):1307-18.
56.Rasul A, Ding C, Li X, Khan M, Yi F, Ali M, Ma T.
Dracorhodin perchlorate inhibits PI3K/Akt and NF-κB activation, up-regulates the expression ofp53, and enhances apoptosis.
Apoptosis. 2012 Oct;17(10):1104-19.
57.Liu Z, Zhao Y, Li J, Xu S, Liu C, Zhu Y, Liang S.
The venom of the spider Macrothele raveni induces apoptosis in the myelogenous leukemia K562cell line.
Leuk Res. 2012 Aug;36(8):1063-6.
58.Xie SQ, Zhang YH, Li Q, Xu FH, Miao JW, Zhao J, Wang CJ.
3-Nitro-naphthalimide and nitrogen mustard conjugate NNM-25 induces hepatocellular carcinoma apoptosis via PARP-1/p53 pathway.
Apoptosis. 2012 Jul;17(7):725-34.
59.Li P, Wang K, Zhang J, Zhao L, Liang H, Shao C, Sutherland LC.
The 3p21.3 tumor suppressor RBM5 resensitizes cisplatin-resistant human non-small cell lungcancer cells to cisplatin.
Cancer Epidemiol. 2012 Oct;36(5):481-9.
60.Lin F, Lin P, Zhao D, Chen Y, Xiao L, Qin W, Li D, Chen H, Zhao B, Zou H, Zheng X, Yu X.
Sox2 targets cyclinE, p27 and survivin to regulate androgen-independent human prostate cancercell proliferation and apoptosis.
Cell Prolif. 2012 Jun;45(3):207-16.
61.Sun T, Zheng W, Peng H, Zhang A, Chen Y, Tan R, Shen P.
A small molecule IFB07188 inhibits proliferation of human cancer cells by inducing G2/M cell cycle arrest and apoptosis.
Biomed Pharmacother. 2012 Oct;66(7):512-8.
62.Zhu S, Wang Y, Jin J, Guan C, Li M, Xi C, Ouyang Z, Chen M, Qiu Y, Huang M, Huang Z.
Endoplasmic reticulum stress mediates aristolochic acid I-induced apoptosis in human renalproximal tubular epithelial cells.
Toxicol In Vitro. 2012 Aug;26(5):663-71.
63.Zhao D, Lin F, Wu X, Zhao Q, Zhao B, Lin P, Zhang Y, Yu X.
Pseudolaric acid B induces apoptosis via proteasome-mediated Bcl-2 degradation in hormone-refractory prostate cancer DU145 cells.
Toxicol In Vitro. 2012 Jun;26(4):595-602.
64.Yang G, Zhou X, Wang J, Zhang W, Zheng H, Lu W, Yuan J.
MEHP-induced oxidative DNA damage and apoptosis in HepG2 cells correlates with p53-mediatedmitochondria-dependent signaling pathway.
Food Chem Toxicol. 2012 Jul;50(7):2424-31.
65.Qi L, Wu P, Zhang X, Qiu Y, Jiang W, Huang D, Liu Y, Tan P, Tian Y.
Inhibiting ERp29 expression enhances radiosensitivity in human nasopharyngeal carcinoma celllines.
Med Oncol. 2012 Jun;29(2):721-8.
66.Shi G, Zhang Z, Zhang R, Zhang X, Lu Y, Yang J, Zhang D, Zhang Z, Li X, Ning G.
Protective effect of andrographolide against concanavalin A-induced liver injury.
Naunyn Schmiedebergs Arch Pharmacol. 2012 Jan;385(1):69-79.
67.Wang C, Sheng G, Lu J, Xie L, Bai S, Wang Y, Liu Y.
Effect of RNAi-induced down regulation of nuclear factor kappa-B p65 on acute monocyticleukemia THP-1 cells in vitro and vivo.
Mol Cell Biochem. 2012 Jan;359(1-2):125-33.
68.Du P, Hu S, Cheng Y, Li F, Li M, Li J, Yi L, Feng H.
Photodynamic therapy leads to death of C6 glioma cells partly through AMPAR.
Brain Res. 2012 Jan 18;1433:153-9.
69.Wang X, Wu H, Chen H, Liu R, Liu J, Zhang T, Yu W, Hai C.
Does insulin bolster antioxidant defenses via the extracellular signal-regulated kinases-protein kinase b-nuclear factor erythroid 2 p45-related factor 2 pathway.
Antioxid Redox Signal. 2012 May 15;16(10):1061-70.
70.Zhang Y, Luo M, Zu Y, Fu Y, Gu C, Wang W, Yao L, Efferth T.
Dryofragin, a phloroglucinol derivative, induces apoptosis in human breast cancer MCF-7 cells through ROS-mediated mitochondrial pathway.
Chem Biol Interact. 2012 Aug 30;199(2):129-36.
71.Liu S, Ma X, Ai Q, Huang Q, Shi T, Zhu M, Wang B, Zhang X.
NOTCH1 functions as an oncogene by regulating the PTEN/PI3K/AKT pathway in clear cell renalcell carcinoma.
Urol Oncol. 2013 Aug;31(6):938-48.
72.Chen T, Hao J, He J, Zhang J, Li Y, Liu R, Li L.
Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest inHepG2 cells.
Food Chem. 2013 Jun 1;138(2-3):1034-41.
73.Chen X, Du Y, Nan J, Zhang X, Qin X, Wang Y, Hou J, Wang Q, Yang J.
Brevilin A, a Novel Natural Product, Inhibits Janus Kinase Activity and Blocks STAT3 Signaling in Cancer Cells.
PLoS One. 2013 May 21;8(5):e63697.
74.Zhang JF, Zhang JG, Kuai XL, Zhang H, Jiang W, Ding WF, Li ZL, Zhu HJ, Mao ZB.
Reactivation of the homeotic tumor suppressor gene CDX2 by 5-aza-2'-deoxycytidine-induced demethylation inhibits cell proliferation and induces caspase-independent apoptosis in gastric cancer cells.
Exp Ther Med. 2013 Mar;5(3):735-741.
75.Du XX, Li YJ, Wu CL, Zhou JH, Han Y, Sui H, Wei XL, Liu L, Huang P, Yuan HH, Zhang TT, Zhang WJ, Xie R, Lang XH, Jia DX, Bai YX.
Initiation of apoptosis, cell cycle arrest and autophagy of esophageal cancer cells by dihydroartemisinin.
Biomed Pharmacother. 2013 Jun;67(5):417-24.
76.Song T, Xue Z, Zhang Z, Shen X, Li X.
Pan-BH3 Mimetic S1 Exhibits Broad-Spectrum Antitumour Effects by Cooperation between Bax and Bak.
Basic Clin Pharmacol Toxicol. 2013 Sep;113(3):145-51.
77.Gu G, Gao X, Hu Q, Kang T, Liu Z, Jiang M, Miao D, Song Q, Yao L, Tu Y, Pang Z, Chen H, Jiang X, Chen J.
The influence of the penetrating peptide iRGD on the effect of paclitaxel-loaded MT1-AF7p-conjugatednanoparticles on glioma cells.
Biomaterials. 2013 Jul;34(21):5138-48.
78.Tang Q, Li G, Wei X, Zhang J, Chiu JF, Hasenmayer D, Zhang D, Zhang H.
Resveratrol-induced apoptosis is enhanced by inhibition of autophagy in esophageal squamous cell carcinoma.
Cancer Lett. 2013 Aug 19;336(2):325-37. 
79.Wang Y, Liu P, Qiu L, Sun Y, Zhu M, Gu L, Di W, Duan Y.
Toxicity and therapy of cisplatin-loaded EGF modified mPEG-PLGA-PLL nanoparticles for SKOV3 cancer in mice.
Biomaterials. 2013 May;34(16):4068-77.
80.Cheng P, Ni Z, Dai X, Wang B, Ding W, Rae Smith A, Xu L, Wu D, He F, Lian J.
The novel BH-3 mimetic apogossypolone induces Beclin-1- and ROS-mediated autophagy in humanhepatocellular cells.
Cell Death Dis. 2013 Feb 7;4:e489.
81.Tang S, Gao D, Zhao T, Zhou J, Zhao X.
An evaluation of the anti-tumor efficacy of oleanolic acid-loaded PEGylated liposomes.
Nanotechnology. 2013 Jun 14;24(23):235102.
82.Zhu K, Guo X, Guo X, Peng W, Zhou H.
Protective effects of wheat germ protein isolate hydrolysates (WGPIH) against hydrogen peroxide-induced oxidative stress in PC12 cells.
Food Research International.2013 Aug;53(1):297-303.
83.Hu Q, Gao X, Gu G, Kang T, Tu Y, Liu Z, Song Q, Yao L, Pang Z, Jiang X, Chen H, Chen J.
Glioma therapy using tumor homing and penetrating peptide-functionalized PEG-PLA nanoparticles loaded withpaclitaxel.
Biomaterials. 2013 Jul;34(22):5640-50.
84.Ding G, Liu H, Wang Y, Lü Y, Wu Y, Guo Y, Xu L.
Fabrication of a magnetite nanoparticle-loaded polymeric nanoplatform for magnetically guided drug delivery.
Chemical Research in Chinese Universities. 2013 Feb;29(1):103-9.
85.Rasul A, Bao R, Malhi M, Zhao B, Tsuji I, Li J, Li X.
Induction of apoptosis by costunolide in bladder cancer cells is mediated through ROS generation andmitochondrial dysfunction.
Molecules. 2013 Jan 24;18(2):1418-33.  
86.Mo R, Sun Q, Li N, Zhang C.
Intracellular delivery and antitumor effects of pH-sensitive liposomes based on zwitterionic oligopeptide lipids.
Biomaterials. 2013 Apr;34(11):2773-86.
87.Zhang X, Li G, Zhang L, Yang D, Zhang Z, Yan A, Linghu H.
C3G overexpression promotes the survival of rat-derived H9C2 cardiomyocytes via p-ERK1/2.
Cell Biol Int. 2013 Oct;37(10):1106-13.
88.Zhou Y, Lan J, Wang W, Shi Q, Lan Y, Cheng Z, Guan H.
ZNRF3 acts as a tumour suppressor by the Wnt signalling pathway in human gastric adenocarcinoma.
J Mol Histol. 2013 Oct;44(5):555-63.
89.Zhao C, Yin P, Mei C, Li N, Yao W, Li X, Qi J, Fan K, Li Z, Wang L, Shi Y, Qiu S, Fan J, Zha X.
Down-regulation of DNA methyltransferase 3B in staurosporine-induced apoptosis and its mechanism in humanhepatocarcinoma cell lines.
Mol Cell Biochem. 2013 Apr;376(1-2):111-9.
90.Zhang Y, Wang H, Lai C, Wang L, Deng Y.
Comparative proteomic analysis of human SH-SY5Y neuroblastoma cells under simulated microgravity.
Astrobiology. 2013 Feb;13(2):143-50.
91.Liu L, Gu L, Ma Q, Zhu D, Huang X.
Resveratrol attenuates hydrogen peroxide-induced apoptosis in human umbilical vein endothelial cells.
Eur Rev Med Pharmacol Sci. 2013 Jan;17(1):88-94.
92.Zhang Y, Rasul A, Li XM, Malhi M, Fang XD,Wang L, Wang Y.
Lentivirus-mediated shRNA targeting decoy receptor 3 (DcR3) inhibits proliferation and augments apoptosis in pancreatic cancer Capan-1 cells.
African Journal of Pharmacy and Pharmacology. 2013 Feb;7(5):203-10.
93.Rasul A, Liu J, Liu Y, Millimouno FM, Wang X, Tsuji I, Yamamura T, Li J, Li XM.
Eriocalyxin B inhibits proliferation and induces apoptosis through downregulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells.
Bangladesh Journal of Pharmacology.2013;8(2):116-23.
94.Wu W, Yang J, Feng X, Wang H, Ye S, Yang P, Tan W, Wei G, Zhou Y.
MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth,migration, and invasion in colorectal carcinoma cells.
Mol Cancer. 2013 Apr 23;12:30. 
95.Chen Z, Rasul A, Zhao C, Millimouno FM.
Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells.
Bangladesh Journal of Pharmacology .2013;8(3):255-262.
96.Zhang T, Yu H, Dong G, Cai L, Bai Y.
Chamaejasmine arrests cell cycle, induces apoptosis and inhibits nuclear NF-κB translocation in the humanbreast cancer cell line MDA-MB-231.
Molecules. 2013 Jan 11;18(1):845-58.
97.Wang B, Yu T, Dong X, Zhang Z, Song L, Xu Y, Zhang XH.
Edwardsiella tarda invasion of fish cell lines and the activation of divergent cell death pathways.
Vet Microbiol. 2013 May 3;163(3-4):282-9.
98.Li D, Ma H, Ye Y, Ji C, Tang X, Ouyang D, Chen J, Li Y, Ma Y.
Deoxynivalenol induces apoptosis in mouse thymic epithelial cells through mitochondria-mediated pathway.
Environ Toxicol Pharmacol. 2014 Jul;38(1):163-71.
99.Yang ZY, Kan JT, Cheng ZY, Wang XL, Zhu YZ, Guo W.
Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway.
Cytotechnology. 2014 Jan;66(1):51-61.
100.Yu XL, Jing T, Zhao H, Li PJ, Xu WH, Shang FF.
Curcumin inhibits expression of inhibitor of DNA binding 1 in PC3 cells and xenografts.
Asian Pac J Cancer Prev. 2014;15(3):1465-70.
101.He Y, Wang N, Shen Y, Zheng Z, Xu X.
Inhibition of high glucose-induced apoptosis by uncoupling protein 2 in human umbilical vein endothelial cells.
Int J Mol Med. 2014 May;33(5):1275-81.
102.Huang L, Huang QY, Huang HQ.
The evidence of HeLa cell apoptosis induced with tetraethylammonium using proteomics and various analytical methods.
J Biol Chem. 2014 Jan 24;289(4):2217-29.
103.Zhang Z, Li J, Yang L, Chen R, Yang R, Zhang H, Cai D, Chen H.
The cytotoxic role of intermittent high glucose on apoptosis and cell viability in pancreatic beta cells.
J Diabetes Res. 2014;2014:712781.
104.Feng Y, Ke C, Tang Q, Dong H, Zheng X, Lin W, Ke J, Huang J, Yeung SC, Zhang H.
Metformin promotes autophagy and apoptosis in esophageal squamous cell carcinoma by downregulating Stat3 signaling.
Cell Death Dis. 2014 Feb 27;5:e1088. 
105.Li CH, Wu DF, Ding H, Zhao Y, Zhou KY, Xu DF.
Berberine hydrochloride impact on physiological processes and modulation of twist levels in nasopharyngeal carcinoma CNE-1 cells.
Asian Pac J Cancer Prev. 2014;15(4):1851-7.
106.Wu L, Li Q, Liu Y.
Polyphyllin D induces apoptosis in K562/A02 cells through G2/M phase arrest.
J Pharm Pharmacol. 2014 May;66(5):713-21. 
107.Hou Y, Lai M, Chen X, Li J, Hu Y, Luo Z, Ding X, Cai K.
Effects of mesoporous SiO2 , Fe3 O4 , and TiO2 nanoparticles on the biological functions of endothelial cells in vitro.
J Biomed Mater Res A. 2014 Jun;102(6):1726-36. 
108.Xie Q, Yang Y, Wang Z, Chen F, Zhang A, Liu C.
Resveratrol-4-O-D-(2'-galloyl)-glucopyranoside isolated from Polygonum cuspidatum exhibits anti-hepatocellular carcinoma viability by inducing apoptosis via the JNK and ERK pathway.
Molecules. 2014 Jan 27;19(2):1592-602.
109.Liu K, Zhang G, Wang Z, Liu Y, Dong J, Dong X, Liu J, Cao J, Ao L, Zhang S.
The protective effect of autophagy on mouse spermatocyte derived cells exposure to 1800MHz radiofrequency electromagnetic radiation.
Toxicol Lett. 2014 Aug 4;228(3):216-24.
110.Liu Y, Li Z, Wu L, Wang Z, Wang X, Yu Y, Zhao Q, Luo F.
MiRNA-125a-5p: a regulator and predictor of gefitinib's effect on nasopharyngeal carcinoma.
Cancer Cell Int. 2014 Mar 7;14(1):24. 
111.Cai H, Meng X, Li Y, Yang C, Liu Y.
Growth Inhibition Effects of Isoalantolactone on K562/A02 Cells: Caspase-dependent Apoptotic Pathways, S Phase Arrest, and Downregulation of Bcr/Abl.
Phytother Res. 2014 Nov;28(11):1679-86.
112.Ran Y, Wu H, Wei L, Yu X, Chen J, Li S, Zhang L, Lou J, Zhu D.
NFATc3 pathway participates in the process that 15-LO/15-HETE protects pulmonary artery smooth muscle cells against apoptosis during hypoxia.
J Recept Signal Transduct Res. 2014 Aug;34(4):270-82. 
113.Huyan T, Li Q, Yang H, Jin ML, Zhang MJ, Ye LJ, Li J, Huang QS, Yin DC.
Protective effect of polysaccharides on simulated microgravity-induced functional inhibition of human NK cells.
Carbohydr Polym. 2014 Jan 30;101:819-27. 
114.Li Q, Liu T, Li Y, Luo S, Zhu Q, Zhang L, Zhao T.
Transport and killing mechanism of a novel camptothecin-deoxycholic acid derivate on hepatocellular carcinoma cells.
J Drug Target. 2014 Jul;22(6):543-52.
115.Li BH, Yin YW, Liu Y, Pi Y, Guo L, Cao XJ, Gao CY, Zhang LL, Li JC.
TRPV1 activation impedes foam cell formation by inducing autophagy in oxLDL-treated vascular smooth muscle cells.
Cell Death Dis. 2014 Apr 17;5:e1182.
116.Zhou Z, Liu Y, Qin M, Sheng W, Wang X, Li Z, Zhong R.
Depletion of PKM2 leads to impaired glycolysis and cell death in 2-demethoxy-2, 3-ethylenediamino hypocrellin B-photoinduced A549 cells.
J Photochem Photobiol B. 2014 May 5;134:1-8.
117.Qu J, Liu W, Huang C, Xu C, Du G, Gu A, Wang X.
Estrogen receptors are involved in polychlorinated biphenyl-induced apoptosis on mouse spermatocyte GC-2 cell line.
Toxicol In Vitro. 2014 Apr;28(3):373-80. 
118.Wang N, Wang X, Yang C, Zhao X, Zhang Y, Wang T, Chen S.
Molecular cloning and multifunctional characterization of GRIM-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (Scophthalmus maximus).
Dev Comp Immunol. 2014 Mar;43(1):96-105.
119.Yang R, Chen D, Li M, Miao F, Liu P, Tang Q.
20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres applied to HeLa cervical cancer cells in vitro.
Biomed Mater Eng. 2014 Jan 1;24(6):1991-8.
120.Fan Y, Chen M, Meng J, Yu L, Tu Y, Wan L, Fang K, Zhu W.
Arsenic trioxide and resveratrol show synergistic anti-leukemia activity and neutralized cardiotoxicity.
PLoS One. 2014 Aug 21;9(8):e105890.
121.Li YJ, Huang P, Jiang CL, Jia de X, Du XX, Zhou JH, Han Y, Sui H, Wei XL, Liu L, Yuan HH, Zhang TT, Zhang WJ, Xie R, Lang XH, Wang LY, Liu T, Bai YX, Tian Y.
Sonodynamically Induced Anti-tumor Effect of 5-Aminolevulinic Acid on Pancreatic Cancer Cells.
Ultrasound Med Biol. 2014 Nov;40(11):2671-9. 
122.Yang X, Zhu H, Ge Y, Liu J, Cai J, Qin Q, Zhan L, Zhang C, Xu L, Liu Z, Yang Y, Yang Y, Ma J, Cheng H, Sun X.
Melittin enhances radiosensitivity of hypoxic head and neck squamous cell carcinoma by suppressing HIF-1α.
Tumour Biol. 2014 Jul 23;255-62.
123.Liang JX, Ning Z, Gao W, Ling J, Wang AM, Luo HF, Liang Y, Yan Q, Wang ZY.
Ubiquitin specific protease 22 induced autophagy is correlated with poor prognosis of pancreatic cancer.
Oncol Rep. 2014 Dec;32(6):2726-34.
124.Wang L, He S, Wu X, Liang S, Mu Z, Wei J, Deng F, Deng Y, Wei S.
Polyetheretherketone/nano-fluorohydroxyapatite composite with antimicrobial activity and osseointegration properties.
Biomaterials. 2014 Aug;35(25):6758-75. 
125.Zhang R, Liu R, Wang W, Xin L, Wang L, Li C, Song L. 
Identification and functional analysis of a novel IFN-like protein (CgIFNLP) in Crassostrea gigas.
Fish Shellfish Immunol. 2015 Jun;44(2):547-54.
126.Qin B, Cao Y, Yang H, Xiao B, Lu Z.
MicroRNA-221/222 regulate ox-LDL-induced endothelial apoptosis via Ets-1/p21 inhibition.
Mol Cell Biochem. 2015 Jul;405(1-2):115-24.
127.Lan QY, Liu QL, Cai J, Liu AW.
3-cinnamoyl-4-hydroxy-6-methyl-2H-pyran-2-one (CHP) inhibits human ovarian cell proliferation by inducing apoptosis.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):155-63. 
128.Yang H, Mao Y, Tan B, Luo S, Zhu Y.
The protective effects of endogenous hydrogen sulfide modulator, S-propargyl-cysteine, on high glucose-induced apoptosis in cardiomyocytes: A novel mechanism mediated by the activation of Nrf2.
Eur J Pharmacol. 2015 Aug 15;761:135-43.
129.Chen G, Qian W, Li J, Xu Y, Chen K.
Exopolysaccharide of Antarctic bacterium Pseudoaltermonas sp. S-5 induces apoptosis in K562 cells.
Carbohydr Polym. 2015 May 5;121:107-14.
130.Xu Y, Shen M, Sun Y, Gao P, Duan Y. 
Polymer Nanocomposites Based Thermo-Sensitive Gel for Paclitaxel and Temozolomide Co-Delivery to Glioblastoma Cells.
J Nanosci Nanotechnol. 2015 Dec;15(12):9777-87.
131.Zhu Z, Song L, He J, Sun Y, Liu X, Zou X.
Ectopic expressed long non-coding RNA H19 contributes to malignant cell behavior of ovarian cancer.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10082-91.
132.Dai J, Wu C, Muneri CW, Niu Y, Zhang S, Rui R, Zhang D.
Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability.
Cryobiology. 2015 Oct;71(2):291-8.
133.Wang Y, Ma L, Wang C, Sheng G, Feng L, Yin C.
Autocrine motility factor receptor promotes the proliferation of human acute monocytic leukemia THP-1 cells.
Int J Mol Med. 2015 Sep;36(3):627-32. 134.Liu Y, Liu Y, Bu W, Xiao Q, Sun Y, Zhao K, Fan W, Liu J, Shi J.
Radiation-/hypoxia-induced solid tumor metastasis and regrowth inhibited by hypoxia-specific upconversion nanoradiosensitizer.
Biomaterials. 2015 May;49:1-8.
135.Xie X, Yang Y, Lin W, Liu H, Liu H, Yang Y, Chen Y, Fu X, Deng J. 
Cell-penetrating peptide-siRNA conjugate loaded YSA-modified nanobubbles for ultrasound triggered siRNA delivery.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:641-50.
136.Li Q, Ye LJ, Ren HL, Huyan T, Li J, Shi JL, Huang QS.
Multiple effects of IL-21 on human NK cells in ex vivo expansion.
Immunobiology. 2015 Jul;220(7):876-88.
137. Ren X, Bai X, Zhang X, Li Z, Tang L, Zhao X, Li Z, Ren Y, Wei S, Wang Q, Liu C, Ji J.
Quantitative nuclear proteomics identifies that miR-137-mediated EZH2 reduction regulates resveratrol-induced apoptosis of neuroblastoma cells.
Mol Cell Proteomics. 2015 Feb;14(2):316-28.
138.Zha X, Wu G, Zhao X, Zhou L, Zhang H, Li J, Ma L, Zhang Y.
PRDX6 Protects ARPE-19 Cells from Oxidative Damage via PI3K/AKT Signaling.
Cell Physiol Biochem. 2015;36(6):2217-28.
139. Zhang R, Shi H, Ren F, Liu H, Zhang M, Deng Y, Li X.
Misregulation of polo-like protein kinase , P53 and P21WAF1 in epithelial ovarian cancer suggests poor prognosis.
Oncol Rep. 2015 Mar;33(3):1235-42.
140.Tang Y, Li J, Li F, Hu CA, Liao P, Tan K, Tan B, Xiong X, Liu G, Li T, Yin Y.
Autophagy protects intestinal epithelial Cells against Deoxynivalenol toxicity by alleviating oxidative stress via IKK signaling pathway.
Free Radic Biol Med. 2015 Dec;89:944-51.
141.Mo M, Zhou M, Wang L, Qi L, Zhou K, Liu LF, Chen Z, Zu XB.
CCL21/CCR7 enhances the proliferation, migration, and invasion of human bladder cancer T24 cells.
PLoS One. 2015 Mar 23;10(3):e0119506.
142.Wang X, Diao Y, Liu Y, Gao N, Gao D, Wan Y, Zhong J, Jin G.
Synergistic apoptosis-inducing effect of aspirin and isosorbide mononitrate on human colon cancer cells.
Mol Med Rep. 2015 Sep;12(3):4750-8.
143.Guo L, Yang X, Lin X, Lin Y, Shen L, Nie Q, Ren L, Guo Q, Que S, Qiu Y.
Silencing of Id2 attenuates hypoxia/ischemia-induced neuronal injury via inhibition of neuronal apoptosis.
Behav Brain Res. 2015 Oct 1;292:528-36.
144.Lv Q, Yang XZ, Fu LY, Lu YT, Lu YH, Zhao J, Wang FJ.
Recombinant expression and purification of a MAP30-cell penetrating peptide fusion protein with higher anti-tumor bioactivity.
Protein Expr Purif. 2015 Jul;111:9-17.
145.Li H, Feng L, Jiang W, Liu Y, Jiang J, Zhang Y, Wu P, Zhou X.
Ca(2+) and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian).
Fish Physiol Biochem. 2015 Oct;41(5):1305-19.
146.Ge H, Tan L, Wu P, Yin Y, Liu X, Meng H, Cui G, Wu N, Lin J, Hu R, Feng H.
Poly-L-ornithine promotes preferred differentiation of neural stem/progenitor cells via ERK signalling pathway.
Sci Rep. 2015 Oct 27;5:15535.
147.Li J, Lei H, Xu Y, Tao ZZ.
miR-512-5p suppresses tumor growth by targeting hTERT in telomerase positive head and neck squamous cell carcinoma in vitro and in vivo.
PLoS One. 2015 Aug 10;10(8):e0135265.
148.Liu XL, Hu JY, Hu MY, Zhang Y, Hong ZY, Cheng XQ, Chen J, Pang DW, Liang Y.
Sequence-dependent abnormal aggregation of human Tau fragment in an inducible cell model.
Biochim Biophys Acta. 2015 Aug;1852(8):1561-73.
149.Meng H, Wang K, Chen X, Guan X, Hu L, Xiong G, Li J, Bai Y.
MicroRNA-330-3p functions as an oncogene in human esophageal cancer by targeting programmed cell death 4.
Am J Cancer Res. 2015 Feb 15;5(3):1062-75. 
150.Lu X, McCoy KS, Xu J, Hu W, Chen H, Jiang K, Han F, Chen P, Wang Y.
Galectin-9 ameliorates respiratory syncytial virus-induced pulmonary immunopathology through regulating the balance between Th17 and regulatory T cells.
Virus Res. 2015 Jan 2;195:162-71.
151.Lin L, Qin Y, Wu H, Chen Y, Wu S, Si X, Wang H, Wang T, Zhong X, Zhai X, Tong L, Pan B, Zhang F, Zhong Z, Wang Y, Zhao W. 
Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin-proteasome system.
Virus Res. 2015 Jan 2;195:207-16.
152.Wang Z, Zeng Q, Chen T, Liao K, Bu Y, Hong S, Hu G.
Silencing NFBD1/MDC1 enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in tumor growth inhibition.
Cell Death Dis. 2015 Aug 6;6:e1849.
153.Sun Y, He W, Luo M, Zhou Y, Chang G, Ren W, Wu K, Li X, Shen J, Zhao X, Hu Y.
SREBP1 regulates tumorigenesis and prognosis of pancreatic cancer through targeting lipid metabolism.
Tumour Biol. 2015 Jun;36(6):4133-41.
154.Wu Z, Li L, Zheng LT, Xu Z, Guo L, Zhen X.
Allosteric modulation of sigma-1 receptors by SKF83959 inhibits microglia-mediated inflammation.
J Neurochem. 2015 Sep;134(5):904-14.
155.Shao XJ, Miao MH, Xue J, Xue J, Ji XQ, Zhu H.
The Down-Regulation of MicroRNA-497 Contributes to Cell Growth and Cisplatin Resistance Through PI3K/Akt Pathway in Osteosarcoma.
Cell Physiol Biochem. 2015;36(5):2051-62.
156.Xiao YC, Yang ZB, Cheng XS, Fang XB, Shen T, Xia CF, Liu P, Qian HH, Sun B, Yin ZF, Li YF.
CXCL8, overexpressed in colorectal cancer, enhances the resistance of colorectal cancer cells to anoikis.
Cancer Lett. 2015 May 28;361(1):22-32.
157.Wang T, Chen T, Niu H, Li C, Xu C, Li Y, Huang R, Zhao J, Wu S.
MicroRNA-218 inhibits the proliferation and metastasis of esophageal squamous cell carcinoma cells by targeting BMI1.
Int J Mol Med. 2015 Jul;36(1):93-102.
158.Fan Y, Weng Z, Gao H, Hu J, Wang H, Li L, Liu H.
Isoalantolactone Enhances the Radiosensitivity of UMSCC-10A Cells via Specific Inhibition of Erk1/2 Phosphorylation.
PLoS One. 2015 Dec 30;10(12):e0145790.
159.Cai Y, Sun Z, Fang X, Fang X, Xiao F, Wang Y, Chen M.
Synthesis, characterization and anti-cancer activity of Pluronic F68-curcumin conjugate micelles.
Drug Deliv. 2015 Jun 16:1-9.
160. Zhang Q, Fan K, Wang P, Yu J, Liu R, Qi H, Sun H, Cao Y.
Carvacrol induces the apoptosis of pulmonary artery smooth muscle cells under hypoxia.
Eur J Pharmacol. 2016 Jan;770(5):134-46.  
161.Wang C, Luo Y, Lu J, Wang Y, Sheng G. 
Polydatin Induces Apoptosis and Inhibits Growth of Acute Monocytic Leukemia Cells.
J Biochem Mol Toxicol. 2016 Apr;30(4):200-5.
162.Li R, Cai L, Tang WJ, Lei C, Hu CM, Yu F. 
Apoptotic Effect of Geniposide on Fibroblast-Like Synoviocytes in Rats with Adjuvant-Induced Arthritis via Inhibiting ERK Signal Pathway In Vitro.
Inflammation. 2016 Feb;39(1):30-8.
163. Li Z, Wang H, Wang Z, Cai H.
MiR-195 inhibits the proliferation of human cervical cancer cells by directly targeting cyclin D1.
Tumour Biol. 2016 May;37(5):6457-63.
164.Tang J, Zhan L, Qin C. 
Inhibition of TLR8 Mediated Signaling Promotes BCG Induced Apoptosis in THP-1 Cells.
Microb Pathog. 2016 Apr;93:78-82.
165.He B, Zhang N, Zhao R. 
Dexamethasone Downregulates SLC7A5 Expression and Promotes Cell Cycle Arrest, Autophagy and Apoptosis in BeWo Cells.
J Cell Physiol. 2016 Jan;231(1):233-42.
166.Zhao C, Su G, Wang X, Zhang X, Guo S, Zhao Y. 
Antitumor activity of ginseng sapogenins, 25-OH-PPD and 25-OCH3-PPD, on gastric cancer cells.
Biotechnol Lett. 2016 Jan;38(1):43-50.  
167.Tu Z, Wang Q, Cui T, Wang J, Ran H, Bao H, Lu J, Wang B, Lydon JP, DeMayo F, Zhang S, Kong S, Wu X, Wang H. 
Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice.
Cell Death Differ. 2016 Jan;23(1):169-81.
168. Peng X, Zhang YY, Wang J, Ji Q.
Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells.
Tumour Biol. 2016 Jan;37(1):865-76.

169.Gao S, Tan H, Zhu N, Gao H, Lv C, Gang J, Ji Y. 
Oridonin induces apoptosis through the mitochondrial pathway in human gastric cancer SGC-7901 cells.
Int J Oncol. 2016 Jun;48(6):2453-60. 
170.Zhang Q, Fan K, Wang P, Yu J, Liu R, Qi H, Sun H, Cao Y. 
Carvacrol induces the apoptosis of pulmonary artery smooth muscle cells under hypoxia.
Eur J Pharmacol. 2016 Jan 5;770:134-46.
171.Gozali MV, Yi F, Zhang JA, Liu J, Wu HJ, Xu Y, Luo D, Zhou BR.
Photodynamic therapy inhibit Fibroblast Growth Factor-10 induced keratinocyte differentiation andproliferation through ROS in Fibroblast Growth Factor Receptor-2b pathway. 
Sci Rep. 2016 Jun 7;6:27402.
172.Gu LL, Zhang XY, Xing WM, Xu JD, Lu H. 
Andrographolide-induced apoptosis in human renal tubular epithelial cells: Roles of endoplasmicreticulum stress and inflammatory response.
Environ Toxicol Pharmacol. 2016 Jul;45:257-64. 
173.Peng W, Wu J, Feng J. 
Long noncoding RNA HULC predicts poor clinical outcome and represents pro-oncogenic activity indiffuse large B-cell lymphoma. 
Biomed Pharmacother. 2016 Apr;79:188-93.
174.Peng X, Zhang YY, Wang J, Ji Q. 
Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells. 
Tumour Biol. 2016 Jan;37(1):865-76. 
175.Wang XD, Li CY, Jiang MM, Li D, Wen P, Song X, Chen JD, Guo LX, Hu XP, Li GQ, Zhang J, Wang CH, He ZD.
Induction of apoptosis in human leukemia cells through an intrinsic pathway by cathachunine, a uniquealkaloid isolated from Catharanthus roseus.
Phytomedicine. 2016 Jun 1;23(6):641-53. 
176.Zhang G, Liu K, Ling X, Wang Z, Zou P, Wang X, Gao J, Yin L, Zhang X, Liu J, Ao L , Cao J. 
DBP-induced endoplasmic reticulum stress in male germ cells causes autophagy, which has acytoprotective role against apoptosis in vitro and in vivo.
Toxicol Lett. 2016 Mar 14;245:86-98. 
177.Bo L, Cui H, Fang Z, Qun T, Xia C. 
Inactivation of transforming growth factor-β-activated kinase 1 promotes taxol efficacy in ovarian cancercells. 
Biomed Pharmacother. 2016 Dec;84:917-924.
178.Tang J, Zhan L, Qin C. 
Inhibition of TLR8 mediated signaling promotes BCG induced apoptosis in THP-1 cells. 
Microb Pathog. 2016 Apr;93:78-82. 
179.Fu JP, Mo WC, Liu Y, He RQ. 
Decline of cell viability and mitochondrial activity in mouse skeletal muscle cell in a hypomagnetic field. 
Bioelectromagnetics. 2016 May;37(4):212-22
180.Zhang Z, Huang W, Lei M, He Y, Yan M, Zhang X, Zhao C. 
Laser-triggered intraocular implant to induce photodynamic therapy for posterior capsule opacificationprevention.
Int J Pharm. 2016 Feb 10;498(1-2):1-11. 
181.Jiao Y, Ma S,Wang Y, Li J, Shan L, Liu Q, Liu Y, Song Q, Yu F, Yu H, Liu H, Huang L, Chen J.
N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced IntrinsicMitochondrial Apoptosis In Vitro in Human Dental Pulp Cells. 
PLoS One. 2016 Jan 25;11(1):e0147858. 
182.Wang X, Zhu P, Pi F, Jiang H, Shao J, Zhang Y, Sun X. 
Sensitive and simple macrophage-based electrochemical biosensor for evaluating lipopolysaccharidecytotoxicity of pathogenic bacteria. 
iosens Bioelectron. 2016 Jul 15;81:349-57. 
183.Liu G, Sheng Y, Zhang M, Sun D.
A polysaccharide from the leaves of Aralia elata induces apoptosis in U-2 OS cells via mitochondrial-dependent pathway.
Int J Biol Macromol. 2016 Dec;93(Pt A):418-425. 
184.Zheng Y, You F, Li Q, Chen J, Yang H.
The effect of geniste on Aβ25-35-induced PC12 cell apoptosis through the JNK-dependent Fas pathway.
ood Funct. 2016 Nov 9;7(11):4702-4708. 
185.Zeng Q, Wang Z, Liu C, Gong Z, Yang L, Jiang L, Ma Z, Qian Y, Yang Y, Kang H, Hong S, Bu Y, Hu G.
Knockdown of NFBD1/MDC1 enhances chemosensitivity to cisplatin or 5-fluorouracil in nasopharyngeal carcinoma CNE1 cells.
Mol Cell Biochem. 2016 Jul;418(1-2):137-46.
186.Li Z, Wang Y, Jiang B, Li W, Zheng L, Yang X, Bao Y, Sun L, Huang Y, Li Y.
Structure, cytotoxic activity and mechanism of protoilludane sesquiterpene aryl esters from themycelium of Armillaria mellea.
J Ethnopharmacol. 2016 May 26;184:119-27.
187.Li K, Xie Y, You M, Huang L, Zheng X.
Plasma sprayed cerium oxide coating inhibits H2O2-induced oxidative stress and supports cell viability.
J Mater Sci Mater Med. 2016 Jun;27(6):100. 
188.Zhang LL, Xu YL, Tang ZH, Xu XH, Chen X, Li T, Ding CY, Huang MQ, Chen XP, Wang YT, Yuan XF, Lu JJ.
Effects of alisol B 23-acetate on ovarian cancer cells: G1 phase cell cycle arrest, apoptosis, migrationand invasion inhibition.
Phytomedicine. 2016 Jul 15;23(8):800-9. 
189.Dong H, Xu J, Li W, Gan J, Lin W, Ke J, Jiang J, Du L, Chen Y, Zhong X, Zhang D, Yeung SJ, Li X, Zhang H. 
Reciprocal androgen receptor/interleukin-6 crosstalk drives oesophageal carcinoma progression andcontributes to patient prognosis. 
Pathol. 2017 Mar;241(4):448-462.

190.Ji J, Zhu P, Sun C, Sun J, An L, Zhang Y, Sun X. 
Pathway of 3-MCPD-induced apoptosis in human embryonic kidney cells.
J Toxicol Sci. 2017;42(1):43-52. 
191.Yin L, Dai Y, Cui Z, Jiang X, Liu W, Han F, Lin A, Cao J, Liu J.
The regulation of cellular apoptosis by the ROS-triggered PERK/EIF2α/chop pathway plays a vital role inbisphenol A-induced male reproductive toxicity.
Toxicol Appl Pharmacol. 2017 Jan 1;314:98-108. 
192.Meng J, Zhang QX, Lu RR. 
Surface layer protein from Lactobacillus acidophilus NCFM inhibit intestinal pathogen-induced apoptosisin HT-29 cells.
Int J Biol Macromol. 2017 Mar;96:766-774. 
193.Li R, Zhang X, Tian X, Shen C, Zhang Q, Zhang Y, Wang Z, Wang F, Tao Y.
Triptolide inhibits tumor growth by induction of cellular senescence.
Oncol Rep. 2017 Jan;37(1):442-448. 
194.Chen X, Lv Q, Liu Y, Deng W.
Effect of Food Additive Citric Acid on The Growth of Human Esophageal Carcinoma Cell Line EC109.
Cell J. 2017 Winter;18(4):493-502.