订货电话
400-1683301
800-8283301
电话订货时间:
周一到周五9:00-17:00
订货邮件
order@beyotime.com
订货QQ
4001683301
微信在线咨询
订单下载
碧云天订单.xls

试剂>

细胞相关>

细胞分析>

细胞死亡

细胞凋亡与坏死检测试剂盒
产品编号: C1056
产品包装: 100次
产品价格: 223.00元
产品简介
使用说明
产品图片
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
C1056 细胞凋亡与坏死检测试剂盒 100次 223.00元

      碧云天生产的细胞凋亡与坏死检测试剂盒(Apoptosis and Necrosis Assay Kit)为您提供了一种经典而又快速简便的细胞凋亡与细胞坏死检测方法。
      本试剂盒采用Hoechst 33342和碘化丙啶(Propidium Iodide,PI)双染的方法。细胞发生凋亡时,染色质会固缩。
Hoechst 33342可以穿透细胞膜,染色后凋亡细胞荧光会比正常细胞明显增强。碘化丙啶(PI)不能穿透细胞膜,对于具有完整细胞膜的正常细胞或凋亡细胞不能染色。而对于坏死细胞,其细胞膜的完整性丧失,碘化丙啶(PI)可以染色坏死细胞。上述两种染料双染后,使用流式细胞仪或荧光显微镜检测时,正常细胞为弱红色荧光+弱蓝色荧光,凋亡细胞为弱红色荧光+强蓝色荧光,坏死细胞为强红色荧光+强蓝色荧光。参考下图,左图为诱导凋亡前的正常细胞,右图为诱导凋亡后的细胞。

      染色快速方便,两种染料的染色仅需20-30分钟,一步染色即可完成。
      使用方便,使用流式细胞仪检测时,无需稀释等配制过程,也无需再准备其它任何溶液。
      本试剂盒足够检测100个样品,每个样品的细胞数量可以为10-100万。
包装清单:

产品编号 产品名称 包装
C1056-1 细胞染色缓冲液 100ml
C1056-2 Hoechst染色液 0.5ml
C1056-3 PI染色液 0.5ml
说明书 1份

保存条件:
      4℃保存六个月有效,-20℃保存一年有效。Hoechst染色液和PI染色液需避光保存。
注意事项:
      需使用流式细胞仪进行红色和蓝色双荧光检测。也可使用荧光显微镜检测。
      染色后宜尽快检测。
      Hoechst 33342对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。碘化丙啶(PI)对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 每个样品收集约10-100万细胞于1.5ml离心管内,离心弃上清。细胞沉淀用0.8-1毫升细胞染色缓冲液重悬。
2. 加入5微升Hoechst染色液。
3. 加入5微升PI染色液。
4. 混匀,冰浴或4℃孵育20-30分钟。
5. 用流式细胞仪检测红色荧光和蓝色荧光。
6. 如果使用荧光显微镜检测,检测前离心沉淀细胞,用PBS洗涤一次,再涂片观察红色荧光和蓝色荧光。对于贴壁细胞使用荧光显微镜检测,可以不收集细胞,直接依次按照上述比例加入细胞染色缓冲液、Hoechst染色液和PI染色液冰浴或4℃染色20-30分钟。染色后PBS洗涤一次,再在荧光显微镜下观察。
相关产品请点击如下按钮:

使用本产品的文献:
1.Ma W, Xu W, Xu H, Chen Y, He Z, Ma M.
Nitric oxide modulates cadmium influx during cadmium-induced programmed cell death intobacco BY-2 cells.
Planta. 2010 Jul;232(2):325-35. 
2.  Jin S, Zhang QY, Kang XM, Wang JX, Zhao WH.
Daidzein induces MCF-7 breast cancer cell apoptosis via the mitochondrial pathway.
Ann Oncol. 2010;21(2):263-8. 
3. Song J, Guo X, Xie X, Zhao X, Li D, Deng W, Song Y, Shen F, Wu M, Wei L.
Autophagy in hypoxia protects cancer cells against apoptosis induced by nutrient deprivationthrough a Beclin1-dependent way in hepatocellular carcinoma.
J Cell Biochem. 2011 Nov;112(11):3406-20.
4. Li G, Ye L, Pan J, Long M, Zhao Z, Yang H, Tian J, Wen Y, Dong S, Guan J, Luo B.
Antitumoural hydroxyapatite nanoparticles-mediated hepatoma-targeted trans-arterialembolization gene therapy: in vitro and in vivo studies.
Liver Int. 2012 Jul;32(6):998-1007.
5. Meng Q, Lv J, Ge H, Zhang L, Xue F, Zhu Y, Liu P.
Overexpressed mutant optineurin(E50K) induces retinal ganglion cells apoptosis via themitochondrial pathway.
Mol Biol Rep. 2012 May;39(5):5867-73.
6. Yi P, Chen G, Zhang H, Tian F, Tan B, Dai J, Wang Q, Deng Z.
Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T.
Biomaterials. 2013 Apr;34(12):3010-9. 
7. Tang S, Hu J, Meng Q, Dong X, Wang K, Qi Y, Chu C, Zhang X, Hou L.
Daidzein induced apoptosis via down-regulation of Bcl-2/Bax and triggering of the mitochondrial pathway in BGC-823 cells.
Cell Biochem Biophys. 2013 Mar;65(2):197-202. 
8. Wang B, Yu T, Dong X, Zhang Z, Song L, Xu Y, Zhang XH.
Edwardsiella tarda invasion of fish cell lines and the activation of divergent cell death pathways.
Vet Microbiol. 2013 May 3;163(3-4):282-9. 
9. Cai XY, Xiong LM, Yang SH, Shao ZW, Xie M, Gao F, Ding F.
Comparison of toxicity effects of ropivacaine, bupivacaine, and lidocaine on rabbit intervertebral disc cells in vitro.
Spine J. 2014 Mar 1;14(3):483-90. 
10.Yin X, Zhou X, Xue Z, Tian L, Zhou Y, Yang L, Zhao D.
Prokaryotic expression and functional analysis of the Mb1514 gene in Mycobacterium bovis.
Mol Cell Biochem. 2014 Jan;385(1-2):43-52.
11.Huang X, Yi C, Fan Y, Zhang Y, Zhao L, Liang Z, Pan J.
Magnetic Fe3O4 nanoparticles grafted with single-chain antibody (scFv) and docetaxel loaded β-cyclodextrin potential for ovarian cancer dual-targeting therapy.
Mater Sci Eng C Mater Biol Appl. 2014 Sep 1;42:325-32. 
12.Zhu W, Yang B, Fu H, Ma L, Liu T, Chai R, Zheng Z, Zhang Q, Li G.
Flavone inhibits nitric oxide synthase (NOS) activity, nitric oxide production and protein S-nitrosylation in breast cancer cells.
Biochem Biophys Res Commun. 2015 Mar 13;458(3):590-5.
13.Chen F, Li Q, Zhang Z, Lin P, Lei L, Wang A, Jin Y.
Endoplasmic Reticulum Stress Cooperates in Zearalenone-Induced Cell Death of RAW 264.7 Macrophages.
Int J Mol Sci. 2015 Aug 20;16(8):19780-95.
14.Sun Y, Zhang J, Zhou J, Huang Z, Hu H, Qiao M, Zhao X, Chen D.
Synergistic effect of cucurbitacin B in combination with curcumin via enhancing apoptosis induction and reversing multidrug resistance in human hepatoma cells.
Eur J Pharmacol. 2015 Dec 5;768:28-40.
15.Han Y, Shao Y, Liu T, Qu YL, Li W, Liu Z. 

Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats.
PLoS One. 2015 Apr 8;10(4):e0122951.
16.Wu J, Zhang E, Fu A. 
A novel cell-permeable RDP-p53 fusion protein for specific inhibition on the growth of cancerous neural cells.
Drug Deliv. 2016;23(7):2464-70.

17.Chen F, Lin P, Wang N, Yang D, Wen X, Zhou D, Wang A, Jin Y. 

Herp depletion inhibits zearalenone-induced cell death in RAW 264.7 macrophages.
Toxicol In Vitro. 2016 Apr;32:115-22. 
18.Wang Y, Wu Y, Wang Y, Fu A, Gong L, Li W, Li Y.
Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1signaling pathway and decreasing ROS production.
Appl Microbiol Biotechnol. 2016 Dec 12. [Epub ahead of print]
19.Wang J, Qi L, Mei L, Wu Z, Wang H C.
butyricum lipoteichoic acid inhibits the inflammatory response and apoptosis in HT-29 cells inducedby S. aureus lipoteichoic acid.
Int J Biol Macromol. 2016 Jul;88:81-7.
20.Chen F, Lin P, Wang N, Yang D, Wen X, Zhou D, Wang A, Jin Y.
Herp depletion inhibits zearalenone-induced cell death in RAW 264.7 macrophages.
Toxicol In Vitro. 2016 Apr;32:115-22.
21.Ma L, Wang H, Wang C, Su J, Xie Q, Xu L, Yu Y, Liu S, Li S, Xu Y, Li Z.
Failure of Elevating Calcium Induces Oxidative Stress Tolerance and Imparts Cisplatin Resistance inOvarian Cancer Cells.
Aging Dis. 2016 May 27;7(3):254-66.
22.Xu X, Sivaramasamy E, Jin S, Li F, Xiang J.
Establishment and characterization of a skin epidermal cell line from mud loach, Misgurnusanguillicaudatus, (MASE) and its interaction with three bacterial pathogens.
Fish Shellfish Immunol. 2016 Aug;55:444-51.
23.Liu W, Wang B, Wang T, Liu X, He X, Liu Y, Li Z, Zeng H.
Ursodeoxycholic Acid Attenuates Acute Aortic Dissection Formation in Angiotensin II-InfusedApolipoprotein E-Deficient Mice Associated with Reduced ROS and Increased Nrf2 Levels.
Cell Physiol Biochem. 2016;38(4):1391-405.
24.Wang Y, Wang X, Shi J, Zhu R, Zhang J, Zhang Z, Ma D, Hou Y, Lin F, Yang J.
Mizuno M A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering.
Sci Rep. 2016 Dec 20;6:39477.
25.Wang Y, Wu Y, Wang Y, Fu A, Gong L, Li W, Li Y.
Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulatingNrf2/Keap1 signaling pathway and decreasing ROS production.
Appl Microbiol Biotechnol. 2017 Apr;101(7):3015-3026.
26.Pang J, Zhou Q, Sun X, Li L, Zhou B, Zeng F, Zhao Y, Shen W, Sun Z.
Effect of low-dose zearalenone exposure on reproductive capacity of male mice.
Toxicol Appl Pharmacol. 2017 Oct 15;333:60-67.
27.Song C, Luo B, Gong L.
Resveratrol reduces the apoptosis induced by cigarette smoke extract by upregulating MFN2.
PLoS One. 2017 Apr 13;12(4):e0175009.
28.Zhang HY, Yang W, Zheng FS, Wang YB, Lu JB.
Long non-coding RNA SNHG1 regulates zinc finger E-box binding homeobox 1 expression by interacting with TAp63 and promotes cell metastasis and invasion in Lung squamous cell carcinoma.
Biomed Pharmacother. 2017 Jun;90:650-658.
29.Jin X, Yu ZF, Chen F, Lu GX, Ding XY, Xie LJ, Sun JT.
Neuronal Nitric Oxide Synthase in Neural Stem Cells Induces Neuronal Fate Commitment via the Inhibition of Histone Deacetylase 2.
Front Cell Neurosci. 2017 Mar 7;11:66.
30.Sun Q, Wang J, Li Y, Zhuang J, Zhang Q, Sun X, Sun D.
Synthesis and evaluation of cytotoxic activities of artemisinin derivatives.
Chem Biol Drug Des. 2017 Nov;90(5):1019-1028.
31.Yi T, Wu X, Long Z, Duan G, Wu Z, Li H, Chen H, Zhou X.
Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 CardiacCells by Binding with Zinc.
Biomed Res Int. 2017;2017:1314297.
32.He W, Huang L, Li M, Yang Y, Chen Z, Shen X.
MiR-148b, MiR-152/ALCAM Axis Regulates the Proliferation and Invasion of Pituitary AdenomasCells.
Cell Physiol Biochem. 2017;44(2):792-803.
33.Long Z, Duan G, Li H, Yi T, Wu X, Chen F, Wu Z, Gao Y.
Ubiquinol-cytochrome c reductase core protein 1 maybe involved in delayed cardioprotection from preconditioning induced by diazoxide.
PLoS One. 2017 Jul 27;12(7):e0181903.
购物车
会员
顶部