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 细胞周期与细胞凋亡检测试剂盒

  产品编号C1052
  产品包装: 50次
  产品价格: 312.00元
产品编号 产品名称 产品包装 产品价格
C1052 细胞周期与细胞凋亡检测试剂盒 50次 312.00元

      碧云天生产的细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)是一种采用经典的碘化丙啶染色
(Propidium staining,即PI staining)方法进行细胞周期与细胞凋亡分析的检测试剂盒。
      碘化丙啶(Propidium,简称PI)是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光,并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后,可以用流式细胞仪对细胞进行DNA含量测定,然后根据DNA含量的分布情况,可以进行细胞周期和细胞凋亡分析。
      碘化丙啶染色后,假设G0/G1期细胞的荧光强度为1,那么含有双份基因组DNA的G2/M期细胞的荧光强度的理论值为2,正在进行DNA复制的S期细胞的荧光强度为1-2之间。凋亡细胞由于细胞核发生浓缩以及发生DNA片段化(DNA fragmentation)导致部分基因组DNA片段在染色过程中丢失,因此凋亡细胞碘化丙啶染色后呈现明显的弱染,即荧光强度小于1,在流式检测的荧光图上出现所谓的sub-G1峰,即凋亡细胞峰。
      细胞发生凋亡时,由于胞浆和染色质浓缩、核碎裂,产生凋亡小体,使细胞的光散射性质发生变化。在细胞凋亡的早期,细胞对前向角光散射的能力显著降低,对侧向光散射的能力增加或没有变化。在细胞凋亡的晚期,前向和侧向光散射的信号均降低。因此可通过流式细胞仪测定细胞光散射的变化观察细胞凋亡情况。
      本试剂盒通常应用于培养的贴壁或悬浮细胞的细胞周期与细胞凋亡检测。如果用于组织的细胞周期与细胞凋亡检测,则必须把组织消化成单细胞状态,才可以进行检测。
      本试剂盒足够检测50个样品,每个样品的细胞数量可以为10-100万。
包装清单:

产品编号 产品名称 包装
C1052-1 染色缓冲液 25ml
C1052-2 碘化丙啶染色液(20X) 1.25ml
C1052-3 RNase A(50X) 0.5ml
说明书 1份

保存条件:
      -20℃保存,一年有效。C1052-2碘化丙啶染色液(20X)需避光保存。本试剂盒可4℃保存,一个月内有效。
注意事项:
      本试剂盒需使用流式细胞仪进行检测。
      需自备PBS和70%乙醇,PBS(C0221A)可以向碧云天订购。
      荧光染料均存在淬灭问题,保存和使用过程中请尽量注意避光,以减缓荧光淬灭。
      碘化丙啶对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 细胞样品的准备:
a. 对于贴壁细胞:小心收集细胞培养液到一离心管内备用。用胰酶消化细胞,至细胞可以被轻轻用移液管或枪头吹打下来时,加
入前面收集的细胞培养液,吹打下所有的贴壁细胞,并轻轻吹散细胞。再次收集到离心管内。1000g左右离心3-5分钟,沉淀细胞。对于特定的细胞,如果细胞沉淀不充分,可以适当延长离心时间或稍稍加大离心力。小心吸除上清,可以残留约50微升左右的培养液,以避免吸走细胞。加入约1毫升冰浴预冷的PBS,重悬细胞,并转移到1.5毫升离心管内。再次离心沉淀细胞,小心吸除上清,可以残留约50微升左右的PBS,以避免吸走细胞。轻轻弹击离心管底以适当分散细胞,避免细胞成团。
b. 对于悬浮细胞:1000g左右离心3-5分钟,沉淀细胞。对于特定的细胞,如果细胞沉淀不充分,可以适当延长离心时间或稍稍
加大离心力。小心吸除上清,可以残留约50微升左右的培养液,以避免吸走细胞。加入约1毫升冰浴预冷的PBS,重悬细胞,并转移到1.5毫升离心管内。再次离心沉淀细胞,小心吸除上清,可以残留约50微升左右的PBS,以避免吸走细胞。轻轻弹击离心管底以适当分散细胞,避免细胞成团。
2. 细胞固定:加入1毫升冰浴预冷70%乙醇中,轻轻吹打混匀,4℃固定2小时或更长时间。固定12-24小时可能效果更佳。
1000g左右离心3-5分钟,沉淀细胞。对于特定的细胞,如果细胞沉淀不充分,可以适当延长离心时间或稍稍加大离心力。小心吸除上清,可以残留约50微升左右的70%乙醇,以避免吸走细胞。加入约1毫升冰浴预冷的PBS,重悬细胞。再次离心沉淀细胞,小心吸除上清,可以残留约50微升左右的PBS,以避免吸走细胞。轻轻弹击离心管底以适当分散细胞,避免细胞成团。
3. 碘化丙啶染色液的配制:参考下表,根据待检测样品的数量配制适量的碘化丙啶染色液:


1个样品 6个样品 12个样品
染色缓冲液 0.5ml 3ml 6ml
碘化丙啶染色液(20X) 25μl 150μl 300μl
RNase A(50X) 10μl 60μl 120μl
Final volume 0.535ml 3.21ml 6.42ml

注:配制好的碘化丙啶染色液短时间内可以4℃保存,宜当日使用。
4. 染色:每管细胞样品中加入0.5毫升碘化丙啶染色液,缓慢并充分重悬细胞沉淀,37℃避光温浴30分钟。随后可以4℃或冰浴避光存放。染色完成后宜在24小时内完成流式检测,最好能在当日完成流式检测。
5. 流式检测和分析:用流式细胞仪在激发波长488nm波长处检测红色荧光,同时检测光散射情况。采用适当分析软件进行细胞
DNA含量分析和光散射分析。

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H2S donor, S-propargyl-cysteine, increases CSE in SGC-7901 and cancer-induced mice:evidencefor a novel anti-cancer effect of endogenous H2S?.
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Xu L, Qi Y, Lv L, Xu Y, Zheng L, Yin L, Liu K, Han X, Zhao Y, Peng J.
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48. Lan QY, Liu QL, Cai J, Liu AW.
3-cinnamoyl-4-hydroxy-6-methyl-2H-pyran-2-one (CHP) inhibits human ovarian cell proliferation by inducing apoptosis.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):155-63.
49. Gao RW, Kong XY, Zhu XX, Zhu GQ, Ma JS, Liu XX.
Retinoic acid promotes primary fetal alveolar epithelial type II cell proliferation and differentiation to alveolar epithelial type I cells.
In Vitro Cell Dev Biol Anim. 2015 May;51(5):479-87.
50. 
Zhang D, Wang J, Wang Z, Zhang T, Shi P, Wang X, Zhao F, Liu X, Lin X, Pang X.
miR-136 modulates TGF-β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes.
Biomed Res Int. 2015;2015:453518. 
51. Hu J, Pan J, Luo Z, Tao Z.
Downregulation of Survivin by shRNA Inhibits Invasion and Enhances the Radiosensitivity of Laryngeal Squamous Cell Carcinoma.
Cell Biochem Biophys.2015 May;72(1):251-7.
52. 
Ma S, Yang LL, Niu T, Cheng C, Zhong L, Zheng MW, Xiong Y, Li LL, Xiang R, Chen LJ, Zhou Q, Wei YQ, Yang SY.
SKLB-67, an FLT3 and Wnt/β-catenin signaling inhibitor, displays potent activity in models of FLT3-driven AML.
Sci Rep. 2015 Oct 26;5:15646. 
53. Zhou B, Shu B, Xi T, Su N, Liu J.
Dub3 expression correlates with tumor progression and poor prognosis in human epithelial ovarian cancer.
Biomed Pharmacother. 2015 Mar;70:84-9.
54. Gao C, Yu Z, Liu S, Xin H, Li X.
Overexpression of CUGBP1 is associated with the progression of non-small cell lung cancer.
Tumour Biol. 2015 Jun;36(6):4583-9.
55. Yu F, Guo Y, Chen B, Dong P, Zheng J.
MicroRNA-17-5p activates hepatic stellate cells through targeting of Smad7.
Lab Invest. 2015 Jul;95(7):781-9.
56. Ye X, Han Y, Zhang L, Liu W, Zuo J.
MTERF4 regulates the mitochondrial dysfunction induced by MPP(+) in SH-SY5Y cells.
Biochem Biophys Res Commun. 2015 Aug 14;464(1):214-20.
57. Chen Y, Zhang W, Huang Y, Gao F, Sha X, Fang X.
Pluronic-based functional polymeric mixed micelles for co-delivery of doxorubicin and paclitaxel to multidrug resistant tumor.
Int J Pharm. 2015 Jul 5;488(1-2):44-58.
58. Meng H, Wang K, Chen X, Guan X, Hu L, Xiong G, Li J, Bai Y.
MicroRNA-330-3p functions as an oncogene in human esophageal cancer by targeting programmed cell death 4.
Am J Cancer Res. 2015 Feb 15;5(3):1062-75.
59. Qian W, Kang L, Zhang T, Meng M, Wang Y, Li Z, Xia Q, Cheng D.
Ecdysone receptor (EcR) is involved in the transcription of cell cycle genes in the silkworm.
Int J Mol Sci.2015 Feb 3;16(2):3335-49.
60. Wang AT, Liang DS, Liu YJ, Qi XR.
Roles of ligand and TPGS of micelles in regulating internalization, penetration and accumulation against sensitive or resistant tumor and therapy for multidrug resistant tumors.
Biomaterials. 2015 Jun;53:160-72.
61. Han Y, Zhao H, Jiang Q, Gao H, Wang C.
Chemopreventive mechanism of polypeptides from Chlamy Farreri (PCF) against UVB-induced malignant transformation of HaCaT cells.
Mutagenesis. 2015 Mar;30(2):287-96.
62. 
Gao L, Wang Y, Xu Z, Li X, Wu J, Liu S, Chu P, Sun Z, Sun B, Lin Y, Peng J, Han G, Wang S, Tang Z.
SZC017, a novel oleanolic acid derivative, induces apoptosis and autophagy in human breast cancer cells.
Apoptosis. 2015 Dec;20(12):1636-50.
63. Zhou B, Yang J, Shu B, Liu K, Xue L, Su N, Liu J, Xi T.
Overexpression of astrocyte-elevated gene-1 is associated with ovarian cancer development and progression.
Mol Med Rep. 2015 Apr;11(4):2981-90.
64.  
Xu T, Ni MM, Xing-Li, Li XF, Meng XM, Huang C, Li J.
NLRC5 regulates TGF-β1-induced proliferation and activation of hepatic stellate cells during hepatic fibrosis.
Int J Biochem Cell Biol. 2016 Jan;70:92-104.
65.
Bao W, Gu Y, Ta L, Wang K, Xu Z.
Induction of autophagy by the MG 132 proteasome inhibitor is associated with endoplasmic reticulum stress in MCF 7 cells.
Mol Med Rep. 2016 Jan;13(1):796-804. 
66.
Wu Y, Shu J, He C, Li M, Wang Y, Ou W, He Y.
ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset induced pluripotent stem cells by suppressing expression and activity of caspase 3.
Theriogenology. 2016 Jan 15;85(2):302-14. 
67.
Liu J, Wang H, Wang B, Chen T, Wang X, Huang P, Xu L, Guo Z.
Microcystin-LR promotes proliferation by activating Akt/S6K1 pathway and disordering apoptosis and cell cycle associated proteins phosphorylation in HL7702 cells.
Toxicol Lett. 2016 Jan 5;240(1):214-25.
68.
Li X, Li T, Chen D, Zhang P, Song Y, Zhu H, Xiao Y, Xing Y.
Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of human prostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway.
Oncol Rep. 2016 Jan;35(1):584-92. 
69. Wei L, Deng W, Cheng Z, Guo H, Wang S, Zhang X, He Y, Tang Q.
Effects of hesperetin on platelet-derived growth factor-BB-induced pulmonary artery smooth muscle cell proliferation.
Mol Med Rep. 2016 Jan;13(1):955-60.
70. Yu LT, Yang MQ, Liu JL, Alfred MO, Li X, Zhang XQ, Zhang J, Wu MY, Wang M, Luo C.
Recombinant Reg3α protein protects against experimental acute pancreatitis in mice.
Mol Cell Endocrinol. 2016 Feb;422(15):150-9. 
71.Huang Z, Huang X, Jiang D, Zhang Y, Huang B, Luo G.
Dihydroartemisinin inhibits cell proliferation by induced G1 arrest and apoptosis in humannasopharyngealcarcinoma cells.
J Cancer Res Ther. 2016 Jan-Mar;12(1):244-7.
72.Li X, Li T, Chen D, Zhang P, Song Y, Zhu H, Xiao Y, Xing Y.
Overexpression of lysine-specific demethylase 1 promotes androgen-independent transition of humanprostate cancer LNCaP cells through activation of the AR signaling pathway and suppression of the p53 signaling pathway.
Oncol Rep. 2016 Jan;35(1):584-92.
73.Wang D, Zhao Y, Wang Y, Rong Y, Qin H, Bao Y, Song Z, Yu C, Sun L, Li Y.
25-methoxyl-dammarane-3β, 12β, 20-triol and artemisinin synergistically inhibit MDA-MB-231 cellproliferation through downregulation of testes-specific protease 50 (TSP50) expression.
Tumour Biol. 2016 Sep;37(9):11805-11813.
74.Liu J, Wang H, Wang B, Chen T, Wang X, Huang P, Xu L, Guo Z.
Microcystin-LR promotes proliferation by activating Akt/S6K1 pathway and disordering apoptosis andcell cycle associated proteins phosphorylation in HL7702 cells.
Toxicol Lett. 2016 Jan 5;240(1):214-25.
75.Mi XG, Song ZB, Sun LG, Bao YL, Yu CL, Wu Y, Li YX.
Cardamonin inhibited cell viability and tumorigenesis partially through blockade of testes-specificprotease 50-mediated nuclear factor-kappaB signaling pathway activation.
Int J Biochem Cell Biol. 2016 Apr;73:63-71.
76.Jiao Y, Ma S, Wang Y, Li J, Shan L, Sun J, Chen J.
Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human DentalPulp Cells via Generation of Oxidative Stress.
Int J Biol Sci. 2016 Mar 21;12(5):580-93.
77.Ding L, Wang S, Wang W, Lv P, Zhao D, Chen F, Meng T, Dong L, Qi L.
Tanshinone IIA Affects Autophagy and Apoptosis of Glioma Cells by Inhibiting Phosphatidylinositol 3-Kinase/Akt/Mammalian Target of Rapamycin Signaling Pathway.
Pharmacology. 2016 Nov 26;99(3-4):188-195.
78.Wu Y, Shu J, He C, Li M, Wang Y, Ou W, He Y.
ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset inducedpluripotent stem cells by suppressing expression and activity of caspase 3.
Theriogenology. 2016 Jan 15;85(2):302-14.
79.Wang Q, Wang Y, Xing Y, Yan Y, Guo P, Zhuang J, Qin F, Zhang J.
Physcion 8-O-β-glucopyranoside induces apoptosis, suppresses invasion and inhibits epithelial tomesenchymal transition of hepatocellular carcinoma HepG2 cells.
Biomed Pharmacother. 2016 Oct;83:372-380.
80.Pang MJ, Yang Z, Zhang XL, Liu ZF, Fan J, Zhang HY.
Physcion, a naturally occurring anthraquinone derivative, induces apoptosis and autophagy in humannasopharyngeal carcinoma.
Acta Pharmacol Sin. 2016 Dec;37(12):1623-1640.
81.Yang JB, Khan M, He YY, Yao M, Li YM, Gao HW, Ma TH.
Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostatecarcinoma cells in vitro.
Acta Pharmacol Sin. 2016 Jul;37(7):950-62.
82.Wang L, Wu X, Huang P, Lv Z, Qi Y, Wei X, Yang P, Zhang F.
JQ, a small molecule inhibitor of BRD, suppresses cell growth and invasion in oral squamous cellcarcinoma.
Oncol Rep. 2016 Oct;36(4):1989-96.
83.Gao H, Liu Y, Li K, Wu T, Peng J, Jing F.
Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellularmatrix metalloproteinase inducer.
Am J Transl Res. 2016 Feb 15;8(2):1115-32.
84.Liang YX, Lu JM, Mo RJ, He HC, Xie J, Jiang FN, Lin ZY, Chen YR, Wu YD, Luo HW, Luo Z, Zhong WD.
E2F1 promotes tumor cell invasion and migration through regulating CD147 in prostate cancer.
Int J Oncol. 2016 Apr;48(4):1650-8.
85.Bao W, Gu Y, Ta L, Wang K, Xu Z.
Induction of autophagy by the MG 132 proteasome inhibitor is associated with endoplasmic reticulum stress in MCF 7 cells.
Mol Med Rep. 2016 Jan;13(1):796-804.
86.Chen PJ, Cai SP, Yang Y, Li WX, Huang C, Meng XM, Li J.
PTP1B confers liver fibrosis by regulating the activation of hepatic stellate cells.
Toxicol Appl Pharmacol. 2016 Feb 1;292:8-18.
87. Zhou DD, Wang X, Wang Y, Xiang XJ, Liang ZC, Zhou Y, Xu A, Bi CH, Zhang L.
MicroRNA-145 inhibits hepatic stellate cell activation and proliferation by targeting ZEB2 through Wnt/β-catenin pathway.
Mol Immunol. 2016 Jul;75:151-60.
88.Liu T, Yuan X, Jia T, Liu C, Ni Z, Qin Z, Yuan Y.
Polymeric prodrug of bufalin for increasing solubility and stability: Synthesis and anticancer study invitro and in vivo.
Int J Pharm. 2016 Jun 15;506(1-2):382-93.
89.Zhang X, Xu J, Jiang T, Liu G, Wang D, Lu Y.
MicroRNA-195 suppresses colorectal cancer cells proliferation via targeting FGF2 and regulating Wnt/β-catenin pathway.
Am J Cancer Res. 2016 Nov 1;6(11):2631-2640.
90.Xu T, Ni MM, Xing-Li, Li XF, Meng XM, Huang C, Li J.
NLRC5 regulates TGF-β1-induced proliferation and activation of hepatic stellate cells during hepaticfibrosis.
Int J Biochem Cell Biol. 2016 Jan;70:92-104.
91.He YH, Li MF, Zhang XY, Meng XM, Huang C, Li J.
NLRC5 promotes cell proliferation via regulating the AKT/VEGF-A signaling pathway in hepatocellularcarcinoma.
Toxicology. 2016 Jun 1;359-360:47-57.
92.Zhang D, Li Y, Wang R, Li Y, Shi P, Kan Z, Pang X.
Inhibition of REST Suppresses Proliferation and Migration in Glioblastoma Cells.
Int J Mol Sci. 2016 May 3;17(5).
93.Xiang Y, Song Y, Li Y, Zhao D, Ma L, Tan L.
miR-483 is Down-Regulated in Polycystic Ovarian Syndrome and Inhibits KGN Cell Proliferation viaTargeting Insulin-Like Growth Factor 1 (IGF1).
Med Sci Monit. 2016 Sep 23;22:3383-3393.
94.Li X, Wu XQ, Xu T, Li XF, Yang Y, Li WX, Huang C, Meng XM, Li J .
Role of histone deacetylases(HDACs) in progression and reversal of liver fibrosis.
Toxicol Appl Pharmacol. 2016 Sep 1;306:58-68.
95.Zhang J, Wang S, Han F, Li J, Yu L, Zhou P, Chen Z, Xue S, Dai C, Li Q.
MicroRNA-542-3p suppresses cellular proliferation of bladder cancer cells through post-transcriptionallyregulating survivin.
Gene. 2016 Apr 1;579(2):146-52.
96.Chang W, Liu M, Xu J, Fu H, Zhou B, Yuan T, Chen P.
MiR-377 inhibits the proliferation of pancreatic cancer by targeting Pim-3.
Tumour Biol. 2016 Nov;37(11):14813-14824.
97.Ren K, Li Y, Lu H, Li Z, Li Z, Wu K, Li Z, Han X.
Long Noncoding RNA HOTAIR Controls Cell Cycle by Functioning as a Competing Endogenous RNA inEsophageal Squamous Cell Carcinoma.
Transl Oncol. 2016 Dec;9(6):489-497.
98.Gu F, Hu C, Tai Z, Yao C, Tian J, Zhang L, Xia Q, Gong C, Gao Y, Gao S.
Tumour microenvironment-responsive lipoic acid nanoparticles for targeted delivery of docetaxel to lungcancer.
Sci Rep. 2016 Nov 2;6:36281.
99.Tu W, Dong C, Konishi T, Kobayashi A, Furusawa Y, Uchihori Y, Xie Y, Dang B, Li W, Shao C.
G(2)-M phase-correlative bystander effects are co-mediated by DNA-PKcs and ATM after carbon ionirradiation.
Mutat Res Genet Toxicol Environ Mutagen. 2016 Jan 1;795:1-6.
100.Mao H, Tao T, Song D, Liu M, Wang X, Liu X, Shi D.
Zedoarondiol Inhibits Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle CellsProliferation via Regulating AMP-Activated Protein Kinase Signaling Pathway.
Cell Physiol Biochem. 2016;40(6):1506-1520.
101.Wei L, Deng W, Cheng Z, Guo H, Wang S, Zhang X, He Y, Tang Q.
Effects of hesperetin on platelet-derived growth factor-BB-induced pulmonary artery smooth muscle cellproliferation.
Mol Med Rep. 2016 Jan;13(1):955-60.
102.Ji S, Ma S, Wang WJ, Huang SZ, Wang TQ, Xiang R, Hu YG, Chen Q, Li LL, Yang SY.
Discovery of selective protein arginine methyltransferase 5 inhibitors and biological evaluations.
Chem Biol Drug Des. 2016 Oct 7. doi: 10.1111/cbdd.12881. [Epub ahead of print]
103.Zhu JJ, Zhang XX, Miao YQ, He SF, Tian DM, Yao XS, Tang JS, Gan Y.
Delivery of acetylthevetin B, an antitumor cardiac glycoside, using polymeric micelles for enhancedtherapeutic efficacy against lung cancer cells.
Acta Pharmacol Sin. 2017 Feb;38(2):290-300.
104.Yang Y, Dong Q, Li R.
Matrine induces the apoptosis of fibroblast-like synoviocytes derived from rats with collagen-inducedarthritis by suppressing the activation of the JAK/STAT signaling pathway.
Int J Mol Med. 2017 Feb;39(2):307-316.
105.Zu G, Yao J, Ji A, Ning S, Luo F, Li Z, Feng D, Rui Y, Li Y, Wang G, Tian X.
Nurr1 promotes intestinal regeneration after ischemia/reperfusion injury by inhibiting the expression ofp21 (Waf1/Cip1).
J Mol Med (Berl).2017 Jan;95(1):83-95.
106.Gang X, Qian W, Zhang T, Yang X, Xia Q, Cheng D.
Aurora B kinase is required for cell cycle progression in silkworm.
Gene. 2017 Jan 30;599:60-67.
107.Chen Q, Weng Z, Lu Y, Jia Y, Ding L, Bai F, Ge M, Lin Q, Wu K.
An Experimental Analysis of the Molecular Effects of Trastuzumab (Herceptin) and Fulvestrant(Falsodex), as Single Agents or in Combination, on Human HR+/HER2+ Breast Cancer Cell Lines and Mouse Tumor Xenografts.
PLoS One. 2017 Jan 3;12(1):e0168960.
108. Xiong N, Li S, Tang K, Bai H, Peng Y, Yang H, Wu C, Liu Y.
Involvement of caveolin-1 in low shear stress-induced breast cancer cell motility and adhesion: Roles ofFAK/Src and ROCK/p-MLC pathways.
Biochim Biophys Acta. 2017 Jan;1864(1):12-22.