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细胞相关> 细胞组织染色> 荧光染色
DiO (细胞膜绿色荧光探针)
产品编号: C1038
产品包装:10mg
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价格: ¥ 472.00
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产品编号 产品名称 产品包装 产品价格
C1038 DiO (细胞膜绿色荧光探针) 10mg 472.00元

DiO即DiOC18(3),全称为3,3′-dioctadecyloxacarbocyanine perchlorate,是最常用的细胞膜荧光探针之一,呈现绿色荧光。DiO是一种亲脂性膜染料,进入细胞膜后可以侧向扩散逐渐使整个细胞的细胞膜被染色。
DiO在进入细胞膜之前荧光非常弱,仅当进入到细胞膜后才可以被激发出很强的荧光。DiO被激发后可以发出绿色的荧光,DiO和磷酯双层膜结合后的激发光谱和发射光谱参考下图。其中,最大激发波长为484nm,最大发射波长为501nm。

DiO的分子式为C53H85ClN2O6,分子量为881.72,CAS number为34215-57-1。
DiO可以溶解于无水乙醇、DMSO和DMF,其中在DMSO中溶解度大于10mg/ml。发现较难溶解时可以适当加热,并用超声处理以促进溶解。
DiO被广泛用于正向或逆向的,活的或固定的神经等细胞或组织的示踪剂或长期示踪剂(long-term tracer)。DiO通常不会明显影响细胞的生存力(viability)。DiO对于细胞膜染色的荧光强度通常要低于DiI,有时对于某些经过固定的组织的染色效果欠佳。
DiO除了最简单的细胞膜荧光标记外,还可以用于检测细胞的融合和粘附,检测发育或移植过程中细胞迁移,通过FRAP(Fluorescence Recovery After Photobleaching)检测脂在细胞膜上的扩散,检测细胞毒性和标记脂蛋白等。
用于细胞膜荧光标记时,DiO的常用浓度为1-30μM,最常用的浓度为5-10μM。DiO可以直接染色活的细胞或组织,染色时间通常为5-20分钟。对于固定的细胞或组织,通常宜使用配制在PBS中的4%多聚甲醛进行固定,使用其它不适当的固定液会导致荧光背景较高。
包装清单:

产品编号 产品名称 包装
C1038 DiO (细胞膜绿色荧光探针) 10mg
说明书 1份

保存条件:
4℃避光保存,一年有效。配制的储存液-20℃避光保存,半年有效。
注意事项:
荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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