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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| C0937-10μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 10μg | 528.00元 |
| C0937-50μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 50μg | 1598.00元 |
| C0937-200μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 200μg | 4898.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 16878 | P09056 | E.coli | ~180aa | 19.9kDa | - |
| About this protein | |
| Name | Recombinant Mouse LIF (Recombinant Mouse Leukemia inhibitory Factor; rmLIF; mLIF); 重组小鼠白血病抑制因子 |
| Synonyms | CDF; D factor; DIA; DI; differentiation inhibitory activity; differentiation stimulating factor; emfilermin; differentiation-stimulating factor; HILDA; cholinergic differentiation factor; leukemia inhibitory factor; melanoma-derived LPL inhibitor; MLPLI; hepatocyte-stimulating factor Ⅲ; rMuLIF; recombinant murine leukemia inhibitory factor |
| Source | Recombinant protein containing the truncated mouse leukemia inhibitory factor is expressed in E. coli. |
| Purity | Sterile, ≥ 95% by SDS-PAGE. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by adding LIF to maintain the morphology and inhibit the differentiation of mouse embryonic stem cell (E14 cell) . And the larger in embryoid body (EB)’s diameter (um) , the stronger biological activity. |
| Physical Appearance | Sterile filtered white lyophilized (freeze-dried) powder |
| Formulation | Lyophilized from a 0.2μM filtered concentrated solution in 1X PBS, pH7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass [1]. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature mouse LIF shares 78% a.a. sequence identity with Human LIF. |
碧云天生产的BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级)，是一种胚胎细胞培养级的重组小鼠LIF蛋白，经验证可以有效维持小鼠胚胎干细胞的增殖和干性。本产品用于培养E14小鼠胚胎干细胞的Embryoid body (EB)的培养状态和效果请参考图1。本产品仅10ng/ml就可以有效维持E14小鼠胚胎干细胞的增殖和干性。
图1.碧云天BeyoEmbryo™ Recombinant Mouse LIF (C0937)用于培养E14小鼠胚胎干细胞第四天时Embryoid body (EB)的效果图。使用本产品培养E14小鼠胚胎干细胞形成EB，并观察EB形态和生长状况。如图所示，空白对照组(Blank)培养第4天时，EB边缘有突起(图A)，细胞开始分化；而加入最终浓度为10ng/ml本产品的实验组(LIF) EB边缘仍然整齐平滑，显示本产品具有抑制E14小鼠胚胎干细胞分化及维持其增殖潜力和多能性的作用。图B和图D分别为图A和图C中单个EB放大的效果。比例尺：100μm。实际检测效果会因细胞、检测仪器和实验条件的不同而存在差异，本图仅供参考。 碧云天BeyoEmbryo™Recombinant Mouse LIF蛋白经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。
碧云天BeyoEmbryo™Recombinant Mouse LIF蛋白经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。
图2.碧云天BeyoEmbryo™Recombinant Mouse LIF (C0937)的SDS-PAGE电泳检测效果图。纯化后的Recombinant Mouse LIF蛋白经BeyoGel™ Plus PAGE预制胶(Tris-Gly, 12%, 15孔) (P0459)电泳，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。图中可见本产品为高纯度的重组蛋白。Marker为BeyoColor™彩色预染分子量标准(10-170kDa) (P0075/P0076/P0077)。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| C0937-10μg | BeyoEmbryo™ Recombinant Mouse LIF | 10μg |
| C0937-50μg | BeyoEmbryo™ Recombinant Mouse LIF | 50μg |
| C0937-200μg | BeyoEmbryo™ Recombinant Mouse LIF | 200μg |
| C0937-1mg | BeyoEmbryo™ Recombinant Mouse LIF | 200μg×5 |
| — | 说明书 | 1份 |
-20℃保存，至少一年有效。
注意事项：蛋白的每次冻融均会引起部分失活，因此首次配制成相应浓度的储存液或工作液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须适当分装后-20℃或更低温度冻存，尽量避免反复冻融。
本产品经过无菌处理，溶解后可以直接用于细胞培养实验，使用过程中请注意无菌操作。
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| C0936-10μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 100μl |
| C0936-50μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 500μl |
| C0936-200μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 2ml |
| C0937-10μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 10μg |
| C0937-50μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 50μg |
| C0937-200μg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 200μg |
| C0937-1mg | BeyoEmbryo™ Recombinant Mouse LIF (胚胎细胞培养级) | 1mg |
| P6033-5μg | Recombinant Murine LIF | 5μg |
| P6033-100μg | Recombinant Murine LIF | 100μg |
| P6033-1mg | Recombinant Murine LIF | 1mg |
| P6339-5μg | Recombinant Rat LIF | 5μg |
| P6339-100μg | Recombinant Rat LIF | 100μg |
| P6339-1mg | Recombinant Rat LIF | 1mg |
| P5354-5μg | Recombinant Human LIF | 5μg |
| P5354-100μg | Recombinant Human LIF | 100μg |
| P5354-1mg | Recombinant Human LIF | 1mg |
| PL695 | Human LIF ELISA Kit | 96次 |
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