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细胞培养

Poly-D-lysine溶液
产品编号: C0312
产品包装: 2mg
产品价格: 109.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
C0312 Poly-D-lysine溶液 2mg 109.00元

      Poly-D-lysine的中文名为多聚赖氨酸,简称PDL。本Poly-D-lysine为Poly-D- lysine hydrobromide,分子式为D-Lys-(D-
Lys)n-D-Lys·xHBr,分子量为150,000-300,000,CAS Number 27964-99-4。
      本产品为已经配制好的Poly-D-lysine溶液,并经过滤除菌处理,可以直接稀释后用于细胞或组织培养方面的实验。
      分子量大于70,000的多聚赖氨酸可以用于促进细胞贴壁生长,本产品可以用于促进细胞的贴壁生长。
包装清单:

产品编号 产品名称 包装
C0312 Poly-D-lysine溶液(5mg/ml) 0.4ml
说明书 1份

保存条件:
      -20℃保存。
注意事项:
      Poly-D-lysine和Poly-L-lysine都可以用于促进细胞的贴壁生长。Poly-L-lysine可以被某些细胞所消化并吸收,摄入过多的Poly-L-lysine会产生一定的细胞毒性。如果遇到Poly-L-lysine有细胞毒性的情况,可以考虑选购Poly-D-lysine。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。


使用说明:
1. 根据实验需要把本Poly-D-lysine溶液稀释至适当浓度溶液后即可使用。不同的细胞,多聚赖氨酸铺被(Coating)的时间和浓度,甚至稀释液的选择有所不同,请自行参考相关文献进行适当的铺被。
2. 多聚赖氨酸用于细胞培养时,较为常用的铺被(Coating)浓度为0.1mg/ml,即可以把本产品稀释50倍后使用。铺被至少5分钟,有些实验需要铺被1-2小时,有些情况则需要铺被过夜。随后吸除多聚赖氨酸溶液,干燥培养器皿,至肉眼观察完全干燥。通风橱内吹风数分钟即可完成干燥,对于有些实验则需要干燥2小时或更长时间。干燥时间较长通常会更加有利于后续的细胞粘附。随后即可直接进行细胞培养,也可以用水、PBS或培养液等适当溶液润洗后再进行细胞培养。
相关产品请点击如下按钮:

使用本产品的文献:
1. Liu Y, Chen S, Li W, Du H, Zhu W.
Isolation and characterization of primary skeletal muscle satellite cells from rats.
Toxicol Mech Methods. 2012 Nov;22(9):721-5.

2.Wang Y, Schachner M.
The intracellular domain of L1CAM binds to casein kinase 2αandis neuroprotective via inhibition ofthe tumorsuppressors PTEN and p53.
J Neurochem. 2015 Jun;133(6):828-43.
3.Huang-Ming Cao, Xiao-Ping Ye, Jun-Hua Ma,He Jiang, Sheng-Xian Li,Rong-Ying Li,Xue-Song Li, Cui-Cui Guo,Zhi-Quan Wang, Ming Zhan, Chun-Lin Zuo,Chun-Ming Pan, Shuang-Xia Zhao, Cui-Xia Zheng,Huai-Dong Song.
Mimecan, a Hormone Abundantly Expressed in Adipose Tissue, Reduced Food Intake Independently of Leptin Signaling.
EBioMedicine.2015;2(11):1718-1724.

4.iang S, Li C, Zhang C, Chen Y, Xu L, Bao C, Wang X, Liu G, Zhang F, Cui D.
CD44v6 Monoclonal Antibody-Conjugated Gold Nanostars for Targeted Photoacoustic Imaging and PlasmonicPhotothermal Therapy of Gastric Cancer Stem-like Cells.
Theranostics. 2015 Jun 1;5(9):970-84.
 
5.Shen C, Cheng W, Yu P, Wang L, Zhou L, Zeng L, Yang Q .
Resveratrol pretreatment attenuates injury and promotes proliferation of neural stem cells followingoxygen-glucose deprivation/reoxygenation by upregulating the expression of Nrf, HO-1 and NQO1 in vitro.
Mol Med Rep. 2016 Oct;14(4):3646-54.
6.Chen D, Zou J, Zong Y, Meng H, An G, Yang L.
Anti-human CD138 monoclonal antibodies and their bispecific formats: generation and characterization.
Immunopharmacol Immunotoxicol. 2016 Jun;38(3):175-83.
7.Wang J, Fan B, Wei Y, Suo X, Ding Y .
A simple multi-well stretching device to induce inflammatory responses of vascular endothelial cells.
Lab Chip. 2016 Jan 21;16(2):360-7.
8.Wang D, Zhao L, Zheng H, Dong M, Pan L, Zhang X, Zhang H, Gao H.
Time-Dependent Lactate Production and Amino Acid Utilization in Cultured Astrocytes UnderHigh Glucose Exposure.
Mol Neurobiol. 2017 Jan 16.
9.Hanxu Ji, Kai Xi, Qiuhong Zhang and Xudong Jia.
Photodegradable hydrogels for external manipulation of cellular microenvironments with real-time monitoring.
RSC Adv., 2017, 7, 24331-24337.
10.Ou-Yang L, Liu Y, Wang BY, Cao P, Zhang JJ, Huang YY, Shen Y, Lyu JX.
Carnosine suppresses oxygen-glucose deprivation/recovery-induced proliferation and migration of reactive astrocytes of rats in vitro.
Acta Pharmacol Sin. 2017 Sep 21.
11.Chen G, Shen Y, Li X, Jiang Q, Cheng S, Gu Y, Liu L, Cao Y.
The endoplasmic reticulum stress inducer thapsigargin enhances the toxicity of ZnOnanoparticles to macrophages and macrophage-endothelial co-culture.
Environ Toxicol Pharmacol. 2017 Mar;50:103-110.
12.Yu Y, Li J, Zhu X, Tang X, Bao Y, Sun X, Huang Y, Tian F, Liu X, Yang L.
Humanized CD7 nanobody-based immunotoxins exhibit promising anti-T-cell acutelymphoblastic leukemia potential.
Int J Nanomedicine. 2017 Mar 13;12:1969-1983.
13.Li J, Chen S, Zhao Z, Luo Y, Hou Y, Li H, He L, Zhou L, Wu W.
Effect of VEGF on Inflammatory Regulation, Neural Survival,and Functional Improvement in Ratsfollowing a Complete Spinal Cord Transection.
Front Cell Neurosci. 2017 Nov 29;11:381.