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台盼蓝染色细胞存活率检测试剂盒
产品编号: C0011
产品包装: 100次
产品价格: 99.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
C0011 台盼蓝染色细胞存活率检测试剂盒 100次 99.00元

      碧云天生产的台盼蓝染色细胞存活率检测试剂盒(Typan Blue Staining Cell Viability Assay Kit),是利用正常的健康细胞能够排斥台盼蓝,而丧失细胞膜完整性的细胞可以被台盼蓝染色研制而成。严格来说,台盼蓝染色检测的是细胞膜的完整性,通常认为细胞膜丧失完整性,即可认为细胞已经死亡。
      台盼蓝染色后,通过显微镜下直接计数或显微镜下拍照后计数,就可以对细胞存活率进行比较精确的定量。台盼蓝染色后的HeLa细胞请参考图1。

      图1. 细胞台盼蓝染色效果图。胰酶消化下来的HeLa细胞,1000g离心1分钟,弃上清,用适量细胞重悬液重悬后,用等体积台盼蓝染色液(2X)染色。箭头所示为台盼蓝染色的死亡细胞。注:本HeLa细胞经过一定的细胞坏死诱导处理。

      台盼蓝染色只需3-5分钟即可完成,并且操作非常简单。
      本试剂盒足够检测100个细胞样品。
包装清单:

产品编号 产品名称 包装
C0011-1 台盼蓝染色液(2X) 10ml
C0011-2 细胞重悬液 100ml
说明书 1份

保存条件:
      4℃保存,一年有效。
注意事项:
      本试剂盒提供的两种溶液都是无菌的,使用时最好在超净台内进行,避免细菌污染。
      本产品长时间存放可能会出现少量颗粒状沉淀,可在37℃水浴约10分钟以充分溶解沉淀。沉淀完全溶解后即可正常使用。       台盼蓝对人体有毒,操作时请特别小心,并注意有效防护以避免直接接触人体或吸入体内。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。


使用说明:
1. 收集细胞:
对于贴壁细胞先用胰酶和/或EDTA消化下细胞。对于悬浮细胞,则可以直接收集细胞。把收集的细胞在1000-2000g离心1分钟,弃上清,用1毫升或根据细胞的量用适当细胞重悬液重新悬起细胞。
2. 台盼蓝染色:
吸取100微升重悬的细胞到一塑料离心管内,加入100微升台盼蓝染色液(2X),轻轻混匀,染色3分钟 (染色3分钟时间已经足够,但染色时间可以更长一些,但不宜超过10分钟)。
注:对于重悬于细胞培养液中的细胞,也可以直接加入等体积的台盼蓝染色液(2X)进行染色。
3. 计数:
吸取少量经过染色的细胞,用血细胞计数板计数。通常如果要比较精确地进行定量,每个细胞样品至少数500个细胞,数出蓝色细胞和细胞总数。
细胞存活率=(细胞总数-蓝色细胞数)/细胞总数×100%
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