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抗体> 常用一抗> 内参抗体
β-Actin Mouse Monoclonal Antibody
产品编号: AF0003
产品包装:100μl
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价格: ¥ 441.00
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产品编号 产品名称 产品包装 产品价格
AF0003 β-Actin Mouse Monoclonal Antibody 100μl 441.00元
来源 用途 交叉反应性 分子量
Mouse WB, ICC, IHC, IF H, M, R 43KDa

WB,Western blot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;ICC,Immunocytochemistry;FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ELISA ChIP
1:1000 - 1:20 1:20 1:20 - - -

抗体详细信息如下:

About this Antibody

Name

β-Actin Mouse Monoclonal Antibody

Category

Monoclonal antibody (mAb); Primary antibody

Isotype

IgG1

Purification

Affinity chromatography

About the Immunogen

Immunogen

synthetic peptide (KLH-coupled)

Gene ID

60/11461/81822

SwissProt

P60709/P60710/P60711

Synonyms

BRWS1; PS1TP5BP1

Category

Cytoskeletal Signaling

Background

Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error.

包装清单:

产品编号 产品名称 包装
AF0003 β-Actin Mouse Monoclonal Antibody 100μl
AZ100 Western一抗稀释液 100ml
说明书 1份

保存条件:
β-Actin Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。 
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测:

a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体 操作可以参考如下网页: http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:

Fig. 1. Western blot analysis on Hela (A), NIH/3T3 (B) and PC12 (C) cell lysatesusing β-actin mousemonoclonal antibody (AF003).

Fig. 2. Immunofluorescent staining of NIH/3T3 cells using β-actin mouse monoclonalantibody (AF0003). Nucleus (blue) were stained with DAPI.

Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissueusing β-actin mouse monoclonal antibody (AF0003).


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