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pCMV-N-CFP
Cat. No.:
Pack size: 100μg 1μg
Price: RMB
Purpose
Express target protein with N-terminal CFP tag in mammalian cell
Depositor
Beyotime
Publication
Sun et al. J Cell Physiol. 2019 Nov;234(11):20623-20633.
Sequence Information
Brief Information
Name Catalog No. Size Description
pCMV-N-CFP D2703-1μg 5003bp DNA solution
Growth In Bacteria
Bacterial Resistance|Kanamycin
Growth Temperature|37℃
Growth Strain|DH5-Alpha
Copy Number|High Copy
Insert Gene Information
Insert gene|N/A
Species|Synthetic
Insert size (bp)|N/A
Gene Sequences|
Promoter|CMV
T-terminal Tag|CFP
C-terminal Tag|N/A
Cloning Method|Restriction Enzyme
5'→3' Cloning Site|BamHⅠ/ApaⅠ
5′ sequencing primer|N-CFP primer 5′-GCACCCAGTCCGCCCTGAGC-3′
3′ sequencing primer | T7 5'-GTAATACGACTCACTATAGGGC- 3'
Backbone
Vector backbone|pCMV
Vector type | Mammalian expresstion
Total vector size| 5003
 
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