Saccharomyces cerevisiae is a widely studied and used eukaryotic model organism, with a genome size of approximately 1.2×107bp, 16 chromosomes in the nucleus, approximately 6000 ORFs, and only 4% of yeast genes containing introns, making its genetic background simple. Saccharomyces cerevisiae exhibits growth characteristics similar to prokaryotes, making it easy to culture and perform genetic manipulations. It can exist in a haploid state, facilitating genotype-phenotype analysis and homologous recombination, thereby enabling easy editing of genome sequences and high-throughput genetic analysis. When expressing exogenous genes, the Saccharomyces cerevisiae expression system possesses certain post-translational processing capabilities, leading to folding and glycosylation modifications of the harvested exogenous proteins to a certain extent, aiding in maintaining protein activity and stability. Additionally, exogenous genes can be secreted and expressed in Saccharomyces cerevisiae, facilitating purification and avoiding excessive accumulation of the expressed products within the cell.
Beyotime Competent Cell Preparation and Transformation Kit for Saccharomyces cerevisiae provides a fast, convenient, and efficient method for preparing highly active competent cells and carrying out plasmid transformation.
Typical Figures
Figure 1. Transformation effect of Beyotime Competent Cell Preparation and Transformation Kit for Saccharomyces cerevisiae (D0310). A. Plate of Saccharomyces cerevisiae INVSc1 competent cells prepared using this kit and transformed with pGAL1,10-α factor-MCS-His-MCS-Flag-URA vector (D2892) after 48 hours of cultivation. B. Gel electrophoresis image of the colonies from Figure A after colony PCR using the Beyotime Yeast Colony PCR Kit by Enzymatic Lysis (D7279).
Product Features
Easy operation: This kit provides all necessary reagents, eliminating the need for dilution and preparation.
Time saving: Competent cells can be prepared within 30 minutes.
Minimizing degradation: This kit provides Carrier DNA, which is a short linear single-stranded DNA that facilitates the entry of the plasmid into yeast cells and protects the plasmid from DNase degradation.
Product Information
| Cat. No. | Product Name | Pack Size | Price |
| D0310S | Competent Cell Preparation and Transformation Kit for Saccharomyces cerevisiae | 50T | |
| D0310M | Competent Cell Preparation and Transformation Kit for Saccharomyces cerevisiae | 250T |