The gel shift assay, also known as electrophoretic mobility shift assay (EMSA) or gel retardation assay, is a highly sensitive experimental technique used to study protein-nucleic acid interactions. The EMSA technique is based on the slower migration rate of protein-DNA or protein-RNA complexes compared to free linear DNA or RNA fragments during non-denaturing polyacrylamide or agarose gel electrophoresis, resulting in a shift in the migration position of DNA or RNA when bound to proteins.

EMSA PAGE Gel Preparation Kit

Beyotime EMSA PAGE Gel Preparation Kit provides all reagents required for preparing EMSA PAGE gels (i.e. polyacrylamide gels) except for distilled water. EMSA PAGE gels with different concentrations can be simply prepared according to the table below.

Ingredients	Volumes (in milliliters) of the required components for preparing different volumes of EMSA PAGE gel.
4% Gel	10	20	30	40	50
TBE Buffer (5X)	1.0	2.0	3.0	4.0	5.0
Ultrapure water	7.6	15.2	22.8	30.4	38.0
40% Acr-Bis (39:1)	1.0	2.0	3.0	4.0	5.0
80% Glycerol	0.313	0.625	0.938	1.250	1.563
10% Gel Polymerization Catalyst	0.075	0.150	0.225	0.300	0.375
TEMED Substitute	0.005	0.010	0.015	0.020	0.025
Ingredients	Volumes (in milliliters) of the required components for preparing different volumes of EMSA PAGE gel.
4.5% Gel	10	20	30	40	50
TBE Buffer (5X)	1.0	2.0	3.0	4.0	5.0
Ultrapure water	7.5	15.0	22.4	29.9	37.4
40% Acr-Bis (39:1)	1.1	2.3	3.4	4.5	5.6
80% Glycerol	0.313	0.625	0.938	1.250	1.563
10% Gel Polymerization Catalyst	0.075	0.150	0.225	0.300	0.375
TEMED Substitute	0.005	0.010	0.015	0.020	0.025
Ingredients	Volumes (in milliliters) of the required components for preparing different volumes of EMSA PAGE gel.
5% Gel	10	20	30	40	50
TBE Buffer (5X)	1.0	2.0	3.0	4.0	5.0
Ultrapure water	7.4	14.7	22.1	29.4	36.8
40% Acr-Bis (39:1)	1.3	2.5	3.8	5.0	6.3
80% Glycerol	0.313	0.625	0.938	1.250	1.563
10% Gel Polymerization Catalyst	0.075	0.150	0.225	0.300	0.375
TEMED Substitute	0.005	0.010	0.015	0.020	0.025
Ingredients	Volumes (in milliliters) of the required components for preparing different volumes of EMSA PAGE gel.
5.5% Gel	10	20	30	40	50
TBE Buffer (5X)	1.0	2.0	3.0	4.0	5.0
Ultrapure water	7.2	14.5	21.7	28.9	36.2
40% Acr-Bis (39:1)	1.4	2.8	4.1	5.5	6.9
80% Glycerol	0.313	0.625	0.938	1.250	1.563
10% Gel Polymerization Catalyst	0.075	0.150	0.225	0.300	0.375
TEMED Substitute	0.005	0.010	0.015	0.020	0.025
Ingredients	Volumes (in milliliters) of the required components for preparing different volumes of EMSA PAGE gel.
6% Gel	10	20	30	40	50
TBE Buffer (5X)	1.0	2.0	3.0	4.0	5.0
Ultrapure water	7.1	14.2	21.3	28.4	35.5
40% Acr-Bis (39:1)	1.5	3.0	4.5	6.0	7.5
80% Glycerol	0.313	0.625	0.938	1.250	1.563
10% Gel Polymerization Catalyst	0.075	0.150	0.225	0.300	0.375
TEMED Substitute	0.005	0.010	0.015	0.020	0.025

Chemiluminescent EMSA Positive Control Kit

Beyotime Chemiluminescent EMSA Positive Control Kit provides positive probe, positive protein, and positive protein antibody for use as a positive control in chemiluminescent EMSA, so as to ensure the normalcy of the experimental conditions and system. This kit is sufficient for a total of 60 reactions, including 15 each for negative control reaction, positive control reaction, unlabeled probe cold competition reaction, and antibody-based Super-shift reaction. It is intended for use with Beyotime Chemiluminescent EMSA Kit (GS009).

Figure 1. Assay results of Beyotime Chemiluminescent EMSA Positive Control Kit (GS010). 1, the negative control reaction; 2, the binding reaction of the positive protein-probe; 3, the unlabelled probe cold competition reaction; 4, the Super-shift reaction with the positive protein antibody. This figure is for reference only, which may vary due to different experimental conditions.

Product Information:

Cat. No.	Product Name	Pack Size	Price
GS298S	EMSA PAGE Gel Preparation Kit	30-50gels
GS010S	Chemiluminescent EMSA Positive Control Kit	60T