Beyotime's Target-TA-Luc Reporter Gene Plasmid Series is developed for the detection of target genes and its mediated activation of signaling pathways. The plasmids are based on Beyotime's pGL6-TA plasmid with multiple target binding sites inserted in its mutiple cloning sites, which can be used to detect the activation level of target genes with high sensitivity.
The pGL6-TA plasmid is a new generation of plasmid designed for the detection of Firefly luciferase reporter gene in mammalian cells. It has the following advantages:
1. The coding region of Firefly luciferase has been improved to ensure better expression in mammalian cells;
2. All possible transcription factor binding sites predicted throughout the plasmid sequence are appropriately mutated to minimize non-specific bindings of transcription factors on the plasmid while keeping the original function unchanged.
The map of Target-TA-Luc plasmid is as follows:
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