BeyoChIP™ ChIP Assay Kit with Protein A/G Magnetic Beads (P2080) is similar to the previously launched ChIP Assay Kit (P2078) in immunoprecipitating chromatin fragments bound to target proteins followed by PCR or Southern blot to identify the precipitated chromatin fragments. The difference is that P2080 product utilizes the Protein A/G beads, while P2078 product employs the Protein A+G Agarose. Both products are suitable for immunoprecipitation of a wide variety of antibodies.
Magnetic bead method is more convenient to use and requires less processing time than agarose method. The conventional agarose method requires time-consuming centrifugation, while the magnetic bead method requires a very short time of magnetic separation, which can effectively ensure the natural state of the immune complexes by avoiding the mechanical shearing on the immune complexes caused by centrifugation.
Product Features:
Wide Applications: This kit employs Protein A/G Magnetic Beads, which is suitable for immunoprecipitation of more types of antibodies including mouse IgG1, IgG2a, IgG2b, IgG3, IgA, rat IgG1, IgG2a, IgG2b, IgG2c, rabbit IgG, rabbit and goat polyclonal Abs, as well as human IgG1, IgG2, IgG3, and IgG4.
High Specificity: The Protein A/G Magnetic Beads provided in this kit are pre-saturated with Salmon Sperm DNA and non-specific binding is greatly reduced.
Convenient Use: Pre-mixed Control Primers are provided in this kit to amplify the partial sequence of human GAPDH.
Based on BeyoChIP™ ChIP Assay Kit with Protein A/G Magnetic Beads (P2080), we also developed BeyoChIP™ Enzymatic ChIP Assay Kit with Protein A/G Magnetic Beads (P2083) which employs the Micrococcal nuclease (MNase) for chromatin fragmentation. Compared to the conventional ultrasonic chromatin fragmentation into several hundred base pairs, the micrococcal nuclease method can fragment DNA to 1-5 nucleosomes in length, which is more accurate and homogeneous than the ultrasonic method. Additionally, the enzymatic fragmentation conditions are milder and easier to optimize, with less damage to DNA and DNA-protein interaction, less protein denaturation. Therefore, the ChIP assay result using P2083 kit is more effective and reproducible.
Typical Figure:
Figure 1. The electrophoresis result of fragmented Hella cell DNA by MNase included in Beyotime's BeyoChIP™ Enzymatic ChIP Assay Kit with Protein A/G Magnetic Beads (P2083) and a widely received similar product from competitor C.
Product Features:
Accurate and homogeneous chromatin fragmentation: Micrococcus nuclease can fragment most DNA to 1-5 nucleosomes in length, which is more accurate and homogeneous than ultrasonic fragmentation method.
Good reproducibility: The enzymatic fragmentation method is milder, with less damage to DNA and DNA-protein binding, less protein denaturation, which is beneficial for subsequent immunoprecipitation with better reproducibility.
High Specificity: The Protein A/G Magnetic Beads provided in this kit are pre-saturated with Salmon Sperm DNA and non-specific binding is greatly reduced.
Full maintenance of immuno-complex activities: The magnetic bead method does not require centrifugation, avoiding the mechanical shearing caused by centrifugation on the immuno-complexes and the activity of the immuno-complexes is fully maintained.
Convenient to Use: Magnetic bead method is more convenient than agarose method, requiring less processing time. The conventional agarose method requires time-consuming centrifugation, while the magnetic bead method requires a very short time of magnetic separation.
Beyotime also provides Micrococcal Nuclease (MNase) (D7201). The enzymatic DNA fragmentation with MNase is milder, generating ChIP with better integrity of nucleosome.
Major advantages of P2083:
✔ MNase cleaves the DNA linker region between nucleosomes, ensuring the integrity of nucleosomes;
✔ MNase enzymatic conditions are milder, eliminating the negative effects of ultrosonic fragmentation such as ultrasound variability and chromatin emulsification;
✔ MNase digestion avoids the destruction of antigenic epitopes due to protein denaturation and degradation caused by ultrasound, enabling effective binding with antibody.
In addition, Beyotime offers ChIP-Seq CRO services (requiring only 15-20 workdays) and ChIP-Seq data analysis service (requiring only 5-10 workdays).
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