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趋化因子

Recombinant Murine CTACK/CCL27
产品编号: P6843-100μg
产品包装: 100μg
产品价格: 2750.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
P6843-100μg Recombinant Murine CTACK/CCL27 100μg 2750.00元
Species Gene ID Accession Source Length MW Tag
Mouse 20301 Q9Z1X0 E. coli 95aa 10.9kDa
About this Antibody
Name Recombinant Murine CTACK/CCL27 (Recombinant Murine Cuteaneous T-cell Attracting Chemokine/CCL27; rMuCTACK/CCL27);Recombinant Mouse CTACK/CCL27 ; 重组小鼠皮肤T细胞虏获趋化因子/CCL27
Synonyms CTACK; ILC; ALP; C-C motif chemokine 27; chemokine (C-C motif) ligand 27; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; ESkine; IL-11 Ralpha-locus chemokine; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; Skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27
Purity >98% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 10-100ng/ml.
Physical Appearance Sterile Filtered lyophilized (freeze-dried) liquid-like powder.
Formulation Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, with 5% trehalose.
Endotoxin Less than 1EU/μg of rMuCTACK/CCL27 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Chemokines
Background CCL27, also named IL-11 R-alpha-locus chemokine (ILC), skinkine, eskine and cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin and it elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 can attract skin-associated memory T-lymphocytes. Studies showed it may play a role in mediating homing of lymphocytes to cutaneous sites and cell migration during embryogenesis. Mature mouse CCL27 is a 95 amino acid (a.a.) protein that shares 57%a.a. and 87%a.a. sequence identity with human and rat CCL27, respectively. It shares 18%~31%a.a. sequence identity with other mouse CC chemokines.
Amino Acid Sequence LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN

包装清单:

产品编号 产品名称 包装
P6843-5μg Recombinant Murine CTACK/CCL27 5μg
P6843-20μg Recombinant Murine CTACK/CCL27 20μg
P6843-100μg Recombinant Murine CTACK/CCL27 100μg
P6843-1mg Recombinant Murine CTACK/CCL27 1mg
- 说明书 1份

保存条件:
       -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
       由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
       本产品在冻干时添加了海藻糖作为保护剂,此时冻干后本产品看起来仍然像是液体,属于正常现象。在冻干工艺上,海藻糖作为常用的冻干保护剂,可显著阻止蛋白质二级结构的改变,防止冻干过程中的蛋白变性。
       本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
       为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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