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细胞因子

Recombinant Murine PDGF-BB
产品编号: P6130-10μg
产品包装: 10μg
产品价格: 1152.00元
产品简介
使用说明
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产品编号 产品名称 产品包装 产品价格
P6130-10μg Recombinant Murine PDGF-BB 10μg 1152.00元
Species Gene ID Accession Source Length MW Tag
Mouse 18591 P31240 E. coli 109aa 24.4kDa
About this Antibody
Name Recombinant Murine PDGF-BB (Recombinant Murine Platelet-derived Growth Factor-BB; rMuPDGF-BB);Recombinant Mouse PDGF-BB ; 重组小鼠血小板衍生生长因子-BB
Synonyms Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Pdgfb; Sis
Purity >98% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 5.0.
Endotoxin Less than 0.1EU/μg of rMuPDGF-BB as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGF A and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant murine PDGF-AA contains 109 amino acids and has a molecular mass of 12.2kDa.
Amino Acid Sequence SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TIVTPRPVT

包装清单:

产品编号 产品名称 包装
P6130-2μg Recombinant Murine PDGF-BB 2μg
P6130-10μg Recombinant Murine PDGF-BB 10μg
P6130-100μg Recombinant Murine PDGF-BB 100μg
P6130-1mg Recombinant Murine PDGF-BB 1mg
- 说明书 1份

保存条件:
       -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
       由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
       本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
       为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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