| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P5921-1mg |
Recombinant Murine IL-5 |
1mg |
16229.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Mouse
|
16191
|
P04401
|
E. coli
|
113aa
|
26.2kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Murine IL-5 (Recombinant Murine Interleukin-5; rMuIL-5)；Recombinant Mouse IL-5 ; 重组小鼠白细胞介素5
|
|
Synonyms
|
BCDF mu; BCGFII; EDF; Eo-CSF; TRF; B-cell differentiation factor I; EDF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRFB cell differentiation factor I
|
|
Purity
|
>98% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, pH9.0, 150mM NaCl.
|
|
Endotoxin
|
Less than 0.1EU/μg of rMuIL-5 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
IL-5, also named B-cell differentiation factor I, eosinophil differentiation factor and TRF, is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. The cDNA for murine IL-5 encodes a signal peptide and a 113 amino acid mature protein. Mature murine IL-5 shares 70%, 94%, 58%, 66%, 59% and 63%a.a. sequence identity with human, rat, canine, equine, feline and porcine IL5, respectively. It shows cross-reactivity with human IL5 receptor.
|
|
Amino Acid Sequence
|
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P5921-5μg
|
Recombinant Murine IL-5
|
5μg
|
|
P5921-25μg
|
Recombinant Murine IL-5
|
25μg
|
|
P5921-100μg
|
Recombinant Murine IL-5
|
100μg
|
|
P5921-1mg
|
Recombinant Murine IL-5
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
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