Beyotime's G-25 series of desalting columns (BeyoDesalt™ G-25 Spin Desalting Column, BeyoDesalt™ G-25 Mini Desalting Column, BeyoDesalt™ G-25 Midi Desalting Column, BeyoDesalt™ G-25 Max Desalting Column) are pre-packed ready-to-use chromatography columns for simple and quick separation of high molecular weight substances from low molecular weight substances. The main application is removing salt ions, detergents, small molecule dyes, buffer agents, and other impurities from samples such as proteins, nucleic acids, peptides, and polysaccharides, which requires only one benchtop centrifuge.
Working Principle
Figure 1. Schematic diagram of the working principle of BeyoDesalt™ Desalting Columns
Product Features
Wide Application:
① Removal of phenol red from the culture medium to facilitate subsequent purification of proteins or nucleic acids by ion exchange chromatography;
② Removal of single nucleotides from DNA samples to facilitate subsequent DNA sequencing; removal of small molecular weight markers from samples; and termination of reactions between large and small molecular weight substances by separation of the two;
③ Removal of protease products, cofactors, or inhibitors;
④ Removal of excess radiolabeling in nucleic acid labeling reactions;
⑤ Removal of excess D-Biotin from in vitro biotin labeling reactions;
Significantly superior to traditional semi-permeable membrane dialysis method
① Fast separation speed, time-saving: The desalting column can quickly separate samples, typically taking only 5 minutes to process one sample, while semi-permeable membrane dialysis typically takes at least 6 hours.
② Suitable for small volume operations, high sample recovery rate: The desalting column can effectively handle small volume samples of 0.1-0.3ml, with a recovery rate usually exceeding 90%. In contrast, during the release of samples after semi-permeable membrane dialysis, some samples may adhere to the membrane and cannot be fully recovered, making it unsuitable for small volume samples.
③ Saves solution: Semi-permeable membrane dialysis requires a large amount of buffer, while this desalting column only requires 10ml of buffer to complete the experiment.
Two using methods: gravity flow & centrifugation
① Gravity flow: The sample flows through the desalting column by gravity, which is simple to operate, with a slightly higher sample recovery rate compared to centrifugation, but the sample will be diluted by approximately 1.4 times.
② Centrifugation: The sample flows through the desalting column under centrifugal force, with a slightly lower sample recovery rate compared to gravity flow, but the sample will not be diluted.
Figure 2. Schematic diagram for using Beyotime BeyoDesalt™ G-25 Spin desalting column (P2613)
Figure 3. Schematic diagram showing the two methods of using BeyoDesalt™ G-25 Mini/Midi/Max desalting column (P2615/P2617/P2619)
Typical Figures
Figure 4. The effect of Beyotime BeyoDesalt™ G-25 Mini desalting column (P2615) using gravity to separate high molecular weight substances from low molecular weight substances.
Figure 5. Effect of Beyotime BeyoDesalt™ G-25 Midi desalting column (P2617).
Figure 6. Effect of Beyotime BeyoDesalt™ G-25 Max desalting column (P2619).
Selection Guide
Beyotime offers many desalting columns. For comparison and selection, please refer to the table below.
| Product Name | BeyoDesalt™ | |||||||
| Classification | Multi Spin (96 well) | Spin | Mini | Midi | Max | Superfine | Fine | |
| Diagram |  |
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| Matrix | Beyodex™ G-25 M Beyorose™ 6FF |
G-25 M 6FF |
G-10 G-25 M 6FF |
G-10 G-25 M 6FF |
G-25 M 6FF |
G-25 SF 6FF |
G-25 F 6FF |
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| Resolution | ★★★ | ★★★★★ | ★★★★ | |||||
| Spin*** | Sample volume | 70-130μl | 0.2-0.5ml | 0.75-1.5ml | 1.75-2.5ml | Not Applicable | ||
| Elution volume | 70-130μl | 0.2-0.5ml | 0.75-1.5ml | 1.75-2.5ml | ||||
| Dilution factor | 1 | |||||||
| Gravity | Sample volume | Not Applicable | 0.1-0.3ml* 0.1-0.5ml** |
0.4-1ml* 0.5-1.5ml** |
1-2.5ml | |||
| Elution volume | 1ml | 2ml | 3.5ml | |||||
| Dilution factor | 2-10 | 1.3-3 | 1.5-3.5 | |||||
| Syringe | Sample volume | Not Applicable | Not Applicable | 0.25-1.5ml | Not Applicable | |||
| Elution volume | 1-2ml | |||||||
| Dilution factor | 1.3-4 | |||||||
| AKTA System |
Sample volume | Not Applicable | Not Applicable | 0.25-1.5ml | 2-20ml | |||
| Elution volume | 1-2ml | 10-30ml | ||||||
| Dilution factor | 1.3-4 | 1.5-5 | ||||||
| Number of columns used in series |
Not Applicable | 1-5 columns | ||||||
Beyotime offers desalting columns with three types of matrix. For comparison and selection based on the molecular weight cut-off (MWCO), please refer to the table below.
| Matrix | MCWO | Catalog Number | ||||||
| Multi Spin | Spin | Mini | Midi | Max | Superfine | Fine | ||
| Beyodex™ G-10 | >0.7kDa | / | / | P2605 | P2607 | / | / | / |
| Beyodex™ G-25 | >5kDa | P2611 | P2613 | P2615 | P2617 | P2619 | P2621 | P2623 |
| Beyorose™ 6FF | >400kDa | P2625 | P2627 | P2629 | P2631 | P2633 | P2635 | P2637 |
The comparison and selection of BeyoDesalt™ G-25 desalting columns can be found in the table below.
| Serial Name | BeyoDesalt™ G-25 | ||||||
| Classification | Multi Spin (96 well) |
Spin | Mini | Midi | Max | Superfine | Fine |
| Cat. No. | P2611 | P2613 | P2615 | P2617 | P2619 | P2621 | P2623 |
| Beads size | 70-250μm | 40-70μm | 40-140μm | ||||
| Volume of resin | 500μl | 500μl | 2.1ml | 5.3ml | 8.3ml | 5ml | 70ml |
| Packed bed dimensions | / | / | 0.9´6.3cm | 1.3´6.6cm | 1.6´8.3cm | 1.6´2.5cm | 2.6´18cm |
| Column volume | 800μl | 800μl | 5ml | 8.5ml | 13.5ml | 5ml | 70ml |
| Maximum sample volume | 130μl | 130μl | 0.5ml | 1.5ml | 2.5ml | 1.5ml | 20ml |
| Recovery | 70-90% | 70-90% | 70-95% | 70-95% | 70-95% | 95% | 95% |
| Desalting capacity | >85% | >90% | >95% | ||||
| Plate/column material | polypropylene | ||||||
| Storage solution | 20% ethanol | ||||||
| Storage temperature | 4-30℃ | ||||||